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J Biomed Mater Res B Appl Biomater ; 104(1): 165-9, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25678029

RESUMO

The aim of this study was to evaluate the immunolocalization of dentin matrix protein (DMP)-1 in human primary teeth treated with different pulp capping materials. Twenty-five primary molars were divided into the following groups: formocresol (FC), calcium hydroxide (CH), mineral trioxide aggregate (MTA), corticosteroid/antibiotic solution + CH (O + CH), and Portland cement (PC), and all received conventional pulpotomy treatment. The teeth at the regular exfoliation period were extracted for histological analysis and immunolocalization of DMP-1. Statistical analysis was performed using the χ(2) test (p < 0.05). Histological analysis revealed statistically significant differences in the comparison among the groups through the use of a score system regarding the presence of hard tissue barrier, odontoblastic layer, and internal resorption, but not regarding pulp calcification. Immunohistochemical analysis showed immunostaining for DMP-1 in groups CH, MTA, O + CH, and PC. Internal resorption was observed in the groups FC and CH. MTA and PC showed pulp repair without inflammation and with the presence of hard tissue barrier. DMP-1 immunostaining was higher for MTA and PC, confirming the reparative and bioinductive capacity of these materials.


Assuntos
Proteínas da Matriz Extracelular/metabolismo , Fosfoproteínas/metabolismo , Agentes de Capeamento da Polpa Dentária e Pulpectomia/farmacologia , Dente Decíduo/metabolismo , Dente Decíduo/patologia , Criança , Feminino , Humanos , Imuno-Histoquímica , Masculino , Agentes de Capeamento da Polpa Dentária e Pulpectomia/efeitos adversos
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