RESUMO

Chitosan/poly(DL-lactide-co-glycolide) (Ch/DL PLG) composite scaffolds were fabricated by freeze-drying lyophilization, and were evaluated and compared for use as a bone regeneration scaffold through measurements of the compression mechanical properties of the porous scaffolds. Also, In vitro cell culture of Sprague-Dawley rat's osteoblasts were used to evaluate the phenotype expression of cells in the scaffolds, characterizing the cellular adhesion, proliferation and alkaline phosphatase activity. The gene expression of osteocalcin, sialoprotein, alkaline phosphatase, Type I collagen and TGFß1 were confirmed in the samples; moreover, it was confirmed, the mineralization by IR spectra and EDS analysis. Our results thus show that Ch/DL PLG scaffolds are suitable for biological applications.

Assuntos

Quitosana/química , Teste de Materiais/métodos , Poliglactina 910/química , Engenharia Tecidual/métodos , Fosfatase Alcalina/biossíntese , Fosfatase Alcalina/metabolismo , Animais , Adesão Celular , Proliferação de Células , Colágeno Tipo I/metabolismo , Liofilização , Masculino , Osteoblastos/metabolismo , Osteocalcina/biossíntese , Fenótipo , Ratos , Ratos Sprague-Dawley , Sialoglicoproteínas/biossíntese , Fator de Crescimento Transformador beta1/metabolismo