RESUMO
Hops is a new culture in Brazil. Tissue culture can be an important technique for rapid hop propagation. This paper aims to characterize responses from different genotypes under different growth regulators through the interrelationship of response variables important to hop in vitro growth. Three genotypes were cultivated in six culture media with different combinations of growth regulators, BAP (6-benzylaminopurine), IAA (3-indolacetic acid) and GA3 (gibberellic acid). The means were compared by orthogonal contrasts and the interrelationship of the response variables was performed by path analysis. American genotypes showed favorable root development under the BAP + IAA combination, while the use of IAA improved shoot development. The origin of genotypes was important for defining the best protocol for in vitro cultivation. The path coefficient showed that the variable number of shoots has stronger direct effect on the number of nodal segments. Additionally, in tissue culture assays, the use of a covariable and proper error distribution significantly increased experimental accuracy.(AU)
O lúpulo é uma nova cultura no Brasil. A cultura de tecidos pode ser uma técnica importante para a propagação rápida do lúpulo. Este artigo tem como objetivo caracterizar respostas de diferentes genótipos sob diferentes reguladores de crescimento através da inter-relação de variáveis de resposta importantes para o crescimento in vitro. Três genótipos foram cultivados em seis meios de cultura com diferentes combinações de reguladores de crescimento, BAP (6-benzilaminopurina), AIA (ácido 3-indolacético) e GA3 (ácido giberélico). As médias foram comparadas por contrastes ortogonais e a inter-relação das variáveis de resposta foi realizada por análise de trilha. Os genótipos americanos apresentaram desenvolvimento radicular favorável sob a combinação BAP + AIA, enquanto o uso do AIA melhorou o desenvolvimento da parte aérea. A origem dos genótipos foi importante para definir o melhor protocolo para o cultivo in vitro. O coeficiente de trilha mostrou que a variável número de brotos tem um efeito direto mais forte no número de segmentos nodais. Adicionalmente, em experimentos com cultura de tecidos, o uso de uma covariável e distribuição de erro adequada aumentou significativamente a precisão experimental.(AU)
Assuntos
Humulus/crescimento & desenvolvimento , Humulus/genética , Reguladores de Crescimento de Plantas/análise , Melhoramento Genético/métodos , Técnicas In VitroRESUMO
Hops is a new culture in Brazil. Tissue culture can be an important technique for rapid hop propagation. This paper aims to characterize responses from different genotypes under different growth regulators through the interrelationship of response variables important to hop in vitro growth. Three genotypes were cultivated in six culture media with different combinations of growth regulators, BAP (6-benzylaminopurine), IAA (3-indolacetic acid) and GA3 (gibberellic acid). The means were compared by orthogonal contrasts and the interrelationship of the response variables was performed by path analysis. American genotypes showed favorable root development under the BAP + IAA combination, while the use of IAA improved shoot development. The origin of genotypes was important for defining the best protocol for in vitro cultivation. The path coefficient showed that the variable number of shoots has stronger direct effect on the number of nodal segments. Additionally, in tissue culture assays, the use of a covariable and proper error distribution significantly increased experimental accuracy.
O lúpulo é uma nova cultura no Brasil. A cultura de tecidos pode ser uma técnica importante para a propagação rápida do lúpulo. Este artigo tem como objetivo caracterizar respostas de diferentes genótipos sob diferentes reguladores de crescimento através da inter-relação de variáveis de resposta importantes para o crescimento in vitro. Três genótipos foram cultivados em seis meios de cultura com diferentes combinações de reguladores de crescimento, BAP (6-benzilaminopurina), AIA (ácido 3-indolacético) e GA3 (ácido giberélico). As médias foram comparadas por contrastes ortogonais e a inter-relação das variáveis de resposta foi realizada por análise de trilha. Os genótipos americanos apresentaram desenvolvimento radicular favorável sob a combinação BAP + AIA, enquanto o uso do AIA melhorou o desenvolvimento da parte aérea. A origem dos genótipos foi importante para definir o melhor protocolo para o cultivo in vitro. O coeficiente de trilha mostrou que a variável número de brotos tem um efeito direto mais forte no número de segmentos nodais. Adicionalmente, em experimentos com cultura de tecidos, o uso de uma covariável e distribuição de erro adequada aumentou significativamente a precisão experimental.
Assuntos
Humulus/crescimento & desenvolvimento , Humulus/genética , Melhoramento Genético/métodos , Reguladores de Crescimento de Plantas/análise , Técnicas In VitroRESUMO
Abstract Hops is a new culture in Brazil. Tissue culture can be an important technique for rapid hop propagation. This paper aims to characterize responses from different genotypes under different growth regulators through the interrelationship of response variables important to hop in vitro growth. Three genotypes were cultivated in six culture media with different combinations of growth regulators, BAP (6-benzylaminopurine), IAA (3-indolacetic acid) and GA3 (gibberellic acid). The means were compared by orthogonal contrasts and the interrelationship of the response variables was performed by path analysis. American genotypes showed favorable root development under the BAP + IAA combination, while the use of IAA improved shoot development. The origin of genotypes was important for defining the best protocol for in vitro cultivation. The path coefficient showed that the variable number of shoots has stronger direct effect on the number of nodal segments. Additionally, in tissue culture assays, the use of a covariable and proper error distribution significantly increased experimental accuracy.
Resumo O lúpulo é uma nova cultura no Brasil. A cultura de tecidos pode ser uma técnica importante para a propagação rápida do lúpulo. Este artigo tem como objetivo caracterizar respostas de diferentes genótipos sob diferentes reguladores de crescimento através da inter-relação de variáveis de resposta importantes para o crescimento in vitro. Três genótipos foram cultivados em seis meios de cultura com diferentes combinações de reguladores de crescimento, BAP (6-benzilaminopurina), AIA (ácido 3-indolacético) e GA3 (ácido giberélico). As médias foram comparadas por contrastes ortogonais e a inter-relação das variáveis de resposta foi realizada por análise de trilha. Os genótipos americanos apresentaram desenvolvimento radicular favorável sob a combinação BAP + AIA, enquanto o uso do AIA melhorou o desenvolvimento da parte aérea. A origem dos genótipos foi importante para definir o melhor protocolo para o cultivo in vitro. O coeficiente de trilha mostrou que a variável número de brotos tem um efeito direto mais forte no número de segmentos nodais. Adicionalmente, em experimentos com cultura de tecidos, o uso de uma covariável e distribuição de erro adequada aumentou significativamente a precisão experimental.
RESUMO
Hops is a new culture in Brazil. Tissue culture can be an important technique for rapid hop propagation. This paper aims to characterize responses from different genotypes under different growth regulators through the interrelationship of response variables important to hop in vitro growth. Three genotypes were cultivated in six culture media with different combinations of growth regulators, BAP (6-benzylaminopurine), IAA (3-indolacetic acid) and GA3 (gibberellic acid). The means were compared by orthogonal contrasts and the interrelationship of the response variables was performed by path analysis. American genotypes showed favorable root development under the BAP + IAA combination, while the use of IAA improved shoot development. The origin of genotypes was important for defining the best protocol for in vitro cultivation. The path coefficient showed that the variable number of shoots has stronger direct effect on the number of nodal segments. Additionally, in tissue culture assays, the use of a covariable and proper error distribution significantly increased experimental accuracy.
O lúpulo é uma nova cultura no Brasil. A cultura de tecidos pode ser uma técnica importante para a propagação rápida do lúpulo. Este artigo tem como objetivo caracterizar respostas de diferentes genótipos sob diferentes reguladores de crescimento através da inter-relação de variáveis de resposta importantes para o crescimento in vitro. Três genótipos foram cultivados em seis meios de cultura com diferentes combinações de reguladores de crescimento, BAP (6-benzilaminopurina), AIA (ácido 3-indolacético) e GA3 (ácido giberélico). As médias foram comparadas por contrastes ortogonais e a inter-relação das variáveis de resposta foi realizada por análise de trilha. Os genótipos americanos apresentaram desenvolvimento radicular favorável sob a combinação BAP + AIA, enquanto o uso do AIA melhorou o desenvolvimento da parte aérea. A origem dos genótipos foi importante para definir o melhor protocolo para o cultivo in vitro. O coeficiente de trilha mostrou que a variável número de brotos tem um efeito direto mais forte no número de segmentos nodais. Adicionalmente, em experimentos com cultura de tecidos, o uso de uma covariável e distribuição de erro adequada aumentou significativamente a precisão experimental.
Assuntos
Reguladores de Crescimento de Plantas , Brasil , Brotos de Planta/genética , Meios de Cultura , GenótipoRESUMO
Hops is a new culture in Brazil. Tissue culture can be an important technique for rapid hop propagation. This paper aims to characterize responses from different genotypes under different growth regulators through the interrelationship of response variables important to hop in vitro growth. Three genotypes were cultivated in six culture media with different combinations of growth regulators, BAP (6-benzylaminopurine), IAA (3-indolacetic acid) and GA3 (gibberellic acid). The means were compared by orthogonal contrasts and the interrelationship of the response variables was performed by path analysis. American genotypes showed favorable root development under the BAP + IAA combination, while the use of IAA improved shoot development. The origin of genotypes was important for defining the best protocol for in vitro cultivation. The path coefficient showed that the variable number of shoots has stronger direct effect on the number of nodal segments. Additionally, in tissue culture assays, the use of a covariable and proper error distribution significantly increased experimental accuracy.
Assuntos
Reguladores de Crescimento de Plantas , Brasil , Meios de Cultura , Genótipo , Brotos de Planta/genéticaRESUMO
Brown trout (Salmo trutta) broodstock from a single population were separated prior to spawning andexposed to two different holding environments: a raceway system and a tank system. Eggs were stripped from females and 13 measures of egg qualitywere collected, analysed individually, combined by principle components analysis into an integrated egg quality score which was validated against egg survival.The multivariate egg quality score (PC1) differed for fish held in the tank and raceway systems. Egg survival, chorion breaking strength and chorion Se concentrations were higher in eggs produced by broodstock held in the tank system compared to those in the raceway system. In contrast, chorion concentrations of P and K were higher in eggs from fish held in the raceway system. The results suggest that brown trout broodstock reared in tank systems produced higher quality eggs compared to trout reared in raceways. Finally, this study also indicates that multivariate statistical analysis can be used to determine egg quality from multiple egg parameters.(AU)
Assuntos
Animais , Salmonidae/anatomia & histologia , Salmonidae/embriologia , Salmonidae/fisiologia , PesqueirosRESUMO
Brown trout (Salmo trutta) broodstock from a single population were separated prior to spawning andexposed to two different holding environments: a raceway system and a tank system. Eggs were stripped from females and 13 measures of egg qualitywere collected, analysed individually, combined by principle components analysis into an integrated egg quality score which was validated against egg survival.The multivariate egg quality score (PC1) differed for fish held in the tank and raceway systems. Egg survival, chorion breaking strength and chorion Se concentrations were higher in eggs produced by broodstock held in the tank system compared to those in the raceway system. In contrast, chorion concentrations of P and K were higher in eggs from fish held in the raceway system. The results suggest that brown trout broodstock reared in tank systems produced higher quality eggs compared to trout reared in raceways. Finally, this study also indicates that multivariate statistical analysis can be used to determine egg quality from multiple egg parameters.
Assuntos
Animais , Salmonidae/anatomia & histologia , Salmonidae/embriologia , Salmonidae/fisiologia , PesqueirosRESUMO
OBJECTIVE: To determine the effectiveness of a two-week breast cancer Community-Based Train-the-Trainer program for Lay Community Health Workers (LCHWs) in Grenada. The curriculum was designed to teach laywomen about the LCHW role, how to conduct a community health assessment, and knowledge about breast cancer. DESIGN AND METHODS: A qualitative research design was used. One focus group interview was performed on 10 LCHWs after the completion of the training. A structured interview guided the discussion. The focus group took place at the same location as the training. Data was analyzed using framework analysis. All participants were female. The majority of the participants were over the age of 30. Half have less than a secondary school education, one a bachelor degree, and three have some college education. RESULT: Four themes emerged from the data: service, ethics, commitment to the program and practical knowledge. The LCHWs reported being satisfied with the training and expressed being appropriately prepared after the two-week training. The focus group showed that the LCHWs understood the principles and methods underlying health assessments, understood the role of the LCHWs and their relationship to their community. The LCHWs discussed how they felt confident in teaching other women about breast health. They understood breast cancer, and felt that the hands-on element of the training was helpful. The LCHWs felt that the positive trainer-trainee relations during the training contributed to their confidence in assuming the LCHW role. CONCLUSIONS: These findings suggest that Train-the-Trainer education programs can be an effective methodology to train LCHWs.
Assuntos
Grupos Focais , Agentes Comunitários de Saúde , Educação em Saúde , Neoplasias da Mama , GranadaRESUMO
BACKGROUND: After de-escalation techniques have failed, restraints, seclusion and/or rapid tranquillization may be used for people whose aggression is due to psychosis. Most coercive acts of health care have not been evaluated in trials. METHOD: People admitted to the emergency room of Instituto Philippe Pinel, Rio de Janeiro, Brazil, whose aggression/agitation was thought due to psychosis and for whom staff were unsure if best to restrict using physical restraints or a seclusion room, were randomly allocated to one or the other and followed up to 14 days. The primary outcomes were 'no need to change intervention early - within 1 h' and 'not restricted by 4 h'. RESULTS: A total of 105 people were randomized. Two-thirds of the people secluded were able to be fully managed in this way. Even taking into account the move out of seclusion into restraints, this study provides evidence that embarking on the less restrictive care pathway (seclusion) does not increase overall time in restriction of some sort [not restricted by 4 h: relative risk 1.09, 95% confidence interval 0.75-1.58; mean time to release: restraints 337.6 (s.d.=298.2) min, seclusion room 316.3 (s.d.=264.5) min, p=0.48]. Participants tended to be more satisfied with their care in the seclusion group (17.0% v. 11.1%) but this did not reach conventional levels of statistical significance (p=0.42). CONCLUSIONS: This study should be replicated, but suggests that opting for the least restrictive option in circumstances where there is clinical doubt does not harm or prolong coercion.
Assuntos
Agressão/psicologia , Isolamento de Pacientes/normas , Transtornos Psicóticos/terapia , Restrição Física/normas , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Transtornos Psicóticos/complicações , Resultado do TratamentoRESUMO
El maltrato al adulto mayor es un fenómeno presente tanto en nuestro país como en el extranjero, del que no existen estadísticas claras y actualizadas que permitan evaluar su impacto personal ni social. No obstante, en Chile, en el actual escenario de transición demográfica, se presume su protagonismo. En los establecimientos de larga estadía para adultos mayores, el maltrato, es una situación real y recurrente. Si bien en este ámbito específico las estadísticas son aun más escasas, los medios de comunicación social, con cierta frecuencia, dan cuenta de casos de maltrato. Aun así, queda sin conocerse la real magnitud del problema, dada la dificultad de su denuncia como también la restringida concepción de daño que tienen las personas cercanas al anciano (su familia), y los agentes de salud (administradores y cuidadores). Mediante una revisión bibliográfica se profundiza en el maltrato en el adulto mayor institucionalizado, en especial en aspectos relativos a su realidad e invisibilidad.
The elder abuse is present both in our country and abroad of that. There are no clear statistics and up-to-date to evaluate its impact and social personal. However, in Chile, in the current stage of demographic transition, presumed its role. In the establishment of long stay for older adults is a real situation and recurrent. Although, in this specific field statistics are even more scarce the means of social communication, with some frequency, realize cases of ill-treatment. Even though, there remains without knowing the real magnitude of the problem given the difficulty of its complaint as well as the restricted conception of damage received by individuals (family), and health-care workers (administrators and caregivers). Through this review, it will develop the abuse in institutionalized older adult, especially their reality and invisibility.