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OBJECTIVES: The objective of this study was to evaluate the influence of voxel size on artifacts arising from the exomass in cone beam computed tomography (CBCT). STUDY DESIGN: An imaging phantom was scanned using 2 CBCT units, each adjusted to 2 voxel sizes: 0.2 and 0.3 mm. From 1 to 3 metal inserts of titanium, cobalt-chromium, or amalgam were placed in the exomass and additional CBCT scans were acquired. Mean voxel gray values were obtained from 16 homogeneous areas of the phantom and averaged, and the standard deviation was calculated to obtain voxel gray value variability. The data were analyzed using 2-way analysis of variance, Tukey, and Dunnett tests (α =.05). RESULTS: Overall, mean voxel gray values and voxel gray value variability did not differ significantly between CBCT scans obtained with voxel sizes of 0.2 and 0.3 mm for either CBCT model tested (P > .05). Despite some exceptions in which significant differences were observed between the 2 voxel sizes (P < .05), the mean voxel gray values and voxel gray value variability resulting from different metal compositions and, in most situations, for different numbers of metal inserts in the exomass were not affected. CONCLUSION: Voxel size has little influence on exomass-related CBCT artifacts.

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This study evaluated the effect of 10% sodium hypochlorite (NaOCl) as deproteinizing agent and storage media on bond strength (BS) of two etch-and-rinse adhesive systems to dentin. Twenty-eight sound extracted human third molars were divided in four groups (n = 7), according to dentin treatment (conventional etching or etching followed by 10% NaOCl application) and adhesive systems (GB-Gluma 2Bond and OS-One-Step). After dentin treatments and adhesive application, a composite block was built-up on dentin surface and teeth were serially sectioned to obtain bonded sticks specimens. The sticks were submitted to three aging conditions: (24H) 24 hr in water (immediate), (SH) 3 hr of NaOCl accelerated-aging or (1Y) 1 year of water storage. Afterward, submitted to microtensile bond strength test (µTBS), failure modes and adhesive interfaces analyzes. Data were analyzed by two-way analysis of variance (ANOVA) and Tukey's test (α = .05). Dentin deproteinization before bonding significantly reduced µTBS for GB-treated group (p < .05), regardless the aging conditions. Water storage for 1 year (1Y) and NaOCl accelerated-aging (SH) decreased µTBS for both adhesives. Yet, the groups stored in NaOCl (SH) exhibited the lowest BS results (p < .05). Bond strength of deproteinized dentin was dependent on the adhesive system composition and NaOCl accelerated-aging promoted decreased bond strength and further degradation than water storage for 1 year.

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Abstract Objective: To investigate whether there is an association between sense of coherence levels and early weaning. Method: This study had a quantitative nature and a cross-sectional design. Factors associated with early weaning (interruption of maternal breastfeeding) were investigated in a sample of 425 women older than 18 years, mothers of children up to 36 months of age who were not twins, with no sensory or motor deficiencies, without distinction of ethnicity or social class. The chi-squared test, with a significance level of 5%, was used to evaluate the association between the dependent variable (early weaning) and the independent variables (socioeconomic, demographic factors and sense of coherence level). Variables with p ≤ 0.20 were tested by the multiple logistic regression model. Odds ratio and the respective 95% confidence intervals were estimated. All statistical tests were performed using the SAS 9.2 software. Results: The results showed that mothers with greater sense of coherence were 1.82 times more likely to maintain breastfeeding for longer periods (p = 0.02). Conclusions: The identification of mothers with low sense of coherence allows the early intervention of health professionals, contributing to decrease the rates of early weaning in the population.

Resumo Objetivo: Investigar se há associação entre níveis de senso de coerência e desmame precoce. Método: O presente estudo tem natureza quantitativa e delineamento transversal. Foram investigados fatores associados ao desmame precoce (interrupção da oferta do leite materno à criança) em uma amostra de 425 mulheres com idade superior a 18 anos, sem distinção de etnia ou classe social, mães de crianças com até 36 meses, que não fossem gêmeos ou apresentassem deficiências sensoriais ou motoras. Foi feito o teste de qui-quadrado, com nível de significância de 5%, para testar a associação entre a variável dependente (desmame precoce) e as variáveis independentes (socioeconômicas, demográficas e nível de senso de coerência). As variáveis que apresentaram p ≤ 0,20 foram testadas no modelo de regressão logística múltipla. Os odds ratio e os respectivos intervalos de 95% de confiança foram estimados. Todos os testes estatísticos foram feitos com o programa SAS 9.2. Resultados: Os resultados apontaram que mães com maior senso de coerência possuem 1,82 vez mais chance de manter o aleitamento por mais tempo (p = 0,02). Conclusões: A identificação de mães com baixo senso de coerência permite a intervenção precoce dos profissionais de saúde, contribui para diminuir as taxas de desmame precoce na população.

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OBJECTIVE: To investigate whether there is an association between sense of coherence levels and early weaning. METHOD: This study had a quantitative nature and a cross-sectional design. Factors associated with early weaning (interruption of maternal breastfeeding) were investigated in a sample of 425 women older than 18 years, mothers of children up to 36 months of age who were not twins, with no sensory or motor deficiencies, without distinction of ethnicity or social class. The chi-squared test, with a significance level of 5%, was used to evaluate the association between the dependent variable (early weaning) and the independent variables (socioeconomic, demographic factors and sense of coherence level). Variables with p≤0.20 were tested by the multiple logistic regression model. Odds ratio and the respective 95% confidence intervals were estimated. All statistical tests were performed using the SAS 9.2 software. RESULTS: The results showed that mothers with greater sense of coherence were 1.82 times more likely to maintain breastfeeding for longer periods (p=0.02). CONCLUSIONS: The identification of mothers with low sense of coherence allows the early intervention of health professionals, contributing to decrease the rates of early weaning in the population.

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OBJECTIVES: To create and validate a questionnaire to evaluate infection control in oral radiology. METHODS: The questionnaire was developed after review of the literature, which included published articles and the biosafety protocols available from healthcare agencies. The initial version of the questionnaire was composed of 14 multiple choice questions and was divided into 3 domains on handwashing, disinfection/protection of surfaces and disinfectant used. Content validity was assessed by two expert committees, which reviewed the content and scope of the questionnaire and the relevance of each item, respectively. Reliability was evaluated using test-retest and internal consistency methods with 115 undergraduate dentistry students. Construct validity was assessed using the known-groups technique and factor analysis. The known-groups technique involved 641 undergraduate dentistry students, 20 PhD students and 15 oral radiology professors. In the factor analysis, 3 radiology technicians also participated in addition to the 641 undergraduates, 20 PhD students and 15 oral radiology professors. RESULTS: The content validity results were found to be satisfactory to excellent for the ordinal variables (intraclass correlation coefficient = 0.722-1.000) and good to great for the yes/no questions (kappa = 0.662-0.913) in terms of reliability and good internal consistency (Cronbach's alpha = 0.88). After a factor analysis, some questions were excluded, and the questions were grouped into new domains. Significant differences were observed between answers from different groups. The final version of the questionnaire was composed of nine domains. CONCLUSIONS: The questionnaire created was found to exhibit good psychometric properties for assessing infection control in oral radiology.

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INTRODUCTION: In this study, we aimed to assess the relationship between normative and perceived orthodontic treatment need associated with quality of life, self-esteem, and self-perception. METHODS: The sample included 248 schoolchildren aged 12 years. The normative aspect of orthodontic treatment was assessed by the Dental Health Component and the Aesthetic Component of the Index of Orthodontic Treatment Need. The subjects were further evaluated for their oral health-related quality of life, self-esteem, and self-perception of oral esthetics. The Aesthetic Component of the Index of Orthodontic Treatment Need was considered as the response variable, and generalized linear models estimated by the GENMOD procedure (release 9.3, 2010; SAS Institute, Cary, NC). Model 1 was estimated with only the intercept, providing the basis for evaluating the reduction in variance in the other models studied; then the variables were tested sequentially, considering P ≤0.05 as the criterion for remaining in the model. RESULTS: In the model, self-perception and self-esteem were statistically significant in relation to the perceived need for treatment. The normative need was significantly associated with the outcome variable and was not influenced by independent variables. CONCLUSIONS: The normative need for orthodontics treatment was not overestimated by the perceived need, and the perceived need was not influenced by sex and the impact on quality of life.

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Hand hygiene is part of standard precautions and it has been emphasized that education of this practice depends on the individual's experience in general hygiene. The Hygiene Inventory (HI-23) assess the domains General Hygiene, in the fields of Hands, Personal, Household and Food. Aim: To make the transcultural adaptation of the HI-23 scale for Portuguese­Brazil and to investigate and describe the hygiene behavior according to the HI-23 scale among dental students. Material and Methods: The cross-cultural adaptation process consisted in five steps: two translations, two back translations, review committee, pre-test with a population sample, and reproducibility and reliability of the instrument adapted. For the intra-examiner reproducibility it was applied kappa statistics. The statistical Cronbach's alpha verified the reliability of the HI-23 scale adapted to Portuguese. The adapted instrument was applied to 292 students of Dentistry from 1st to 5th year of graduation from a Faculty of Dentistry. Results: There was good reproducibility (0.43≥κ≥0.81) and good internal consistency (Cronbach's alpha=0.75) for the questions of the questionnaire. The adapted instrument applied among academics, showed that students have good and adequate hygiene pattern for most questions, except for the hygiene in the preparation of food and utensils used to prepare them, in the time spent washing hands, and cleanliness regarding the exchange of clothing (pants/skirt). Conclusion: The transcultural adaptation of the instrument for the Brazilian version was successfully obtained. The instrument can be safely applied to measure the profile of hygiene among professional of the health area.

A higiene das mãos é parte das precauções padrão e tem sido enfatizado que a educação dessa prática depende da experiência individual em higiene geral. O Inventario em Higiene (HI-23) avalia os domínios Higiene Geral, Mãos, Pessoal, Doméstica e na Alimentação. Objetivo: Realizar a adaptação transcultural da escala HI-23 para o português-Brasil e investigar e descrever o comportamento em higiene segundo o HI-23, entre acadêmicos de Odontologia. Material e Métodos: O processo de adaptação transcultural consistiu em cinco etapas: duas traduções; duas retrotraduções, comitê de revisão, pré-teste com uma amostra da população alvo, reprodutibilidade e confiabilidade do instrumento adaptado. Para a reprodutibilidade intraexaminador foi aplicada a estatística kappa. A estatística alfa de Cronbach verificou a confiabilidade da escala HI-23 adaptada para o português. O instrumento adaptado foi aplicado a 292 estudantes de Odontologia do 1º ao 5º ano do curso de graduação de uma Faculdade de Odontologia. Resultados: Houve boa reprodutibilidade (0,43≥k≥0,81) e boa consistência interna (alfa de Cronbach=0,75) para as perguntas do questionário. O instrumento adaptado aplicado entre os acadêmicos, mostrou que os estudantes possuem um padrão de higiene bom e adequado para a maioria das questões, com exceção da higiene no preparo de alimentos e utensílios utilizados para prepará-los, no tempo gasto para lavagem das mãos e no asseio quanto a troca de vestimenta (calça/saia). Conclusão: A adaptação do instrumento para a versão brasileira foi obtida com sucesso. O instrumento pode ser aplicado com segurança para medir o perfil em higiene entre profissionais da área da saúde.

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AIM: In the present study, we evaluated the efficacy of bleaching on enamel and opposite dentin surfaces using 35% hydrogen peroxide (HP) with the prior application of a desensitizing agent. METHODS: Thirty bovine dental fragments, with thicknesses of 1 mm enamel and 1.75 mm dentin, were stained in a solution of black tea. The fragments were randomly divided into three groups (n = 10) according to the following protocols: (a) 2% neutral fluoride + bleaching; (b) desensitizing agent + bleaching; and (c) without desensitizer + bleaching. The bleaching efficacy was evaluated four times: after staining with tea (baseline) and after each of the 3 weeks of bleaching, by means of the CIE Lab method using a reflectance spectrophotometer. The data coordinate L* was evaluated by an analysis of repeated measures with PROC MIXED and Tukey-Kramer's test. The ΔE values were subjected to anova and Tukey's test (α = 0.05). RESULTS: The 35% HP treatment showed greater efficacy on deep dentin after removal of the enamel stains, with increasing means during all times in all treatments. CONCLUSION: The use of a desensitizing agent prior to the bleaching session did not affect the mechanism of action of 35% HP with regard to tooth depth.

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BACKGROUND: It is known that periodontal ligament (PDL) harbors a heterogeneous progenitor cell population at different stages of lineage commitment. However, characterization of PDL stem cells committed to osteoblast/cementoblast (O/C) differentiation remains to be elucidated. The present study is carried out to isolate single cell-derived, cluster of differentiation (CD)105-positive PDL clones and to characterize the clones that present high potential to differentiate toward O/C phenotype in vitro. METHODS: Isolation of single cell-derived colonies (clones) from a CD105-enriched PDL progenitor cell population was performed by the ring-cloning technique. Cell clones were evaluated for their O/C differentiation potential, metabolic activity, and expression of STRO-1 protein. Additionally, the clones that showed potential to O/C differentiation were characterized by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) for expression of runt-related transcriptor factor 2 (RUNX2), alkaline phosphatase, CD105, and CD166 during osteogenic induction. RESULTS: Six PDL-CD105(+) clones were obtained, three being highly O/C clones (C-O) and three others that did not have the ability to produce mineralized matrix in vitro (C-F). The C-O group showed lower metabolic activity compared with the C-F group, and both cell groups were positively immunostained for STRO-1. qRT-PCR analysis demonstrated an increased expression of transcripts for RUNX2 and CD166 during the maturation of C-O cells toward O/C phenotype. CONCLUSIONS: These results provide evidence that PDL-CD105(+) purified progenitor cells comprise a heterogeneous cell population that presents a cell subset with high O/C potential and, further, that surface antigen CD166 is modulated during the O/C maturation of this cell subset.

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