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INTRODUCTION: The objective of this study was to assess the presence of voids in mesial root canals of mandibular molar teeth obturated by using the single-cone (SC) and continuous wave of condensation (CWC) obturation techniques, and results were analyzed by using micro-computed tomography. METHODS: Twenty-four mandibular molars with fully developed roots and mesial root curvature ranging from 25° to 35° were instrumented by using Reciproc R25 files, and then they were obturated by using the SC and CWC techniques. Specimens were scanned before and after obturation for micro-computed tomography analysis (voxel size, 17.42 µm). After volumetric analysis and tridimensional reconstruction of the root canals, data were analyzed by using analysis of variance and the Tukey test. RESULTS: No significant differences were observed between the 2 techniques in terms of total percentage volume of voids: CWC = 3.91% ± 0.72%; SC = 6.52% ± 1.16% (P > .05). Only in the cervical third, CWC showed a significantly lower percentage of voids when compared with SC, namely 2.86% ± 0.94% vs 8.00% ± 1.86%, respectively (P < .05). CONCLUSIONS: The percentage volume of voids was similar in the 2 groups and was influenced by the obturation technique only in the cervical third.

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ABSTRACT Objectives This study evaluated the antimicrobial efficacy of ozone therapy in teeth contaminated with Pseudomonas aeruginosa, Enterococcus faecalis, and Staphylococcus aureus using a mono-species biofilm model. Parallel to this, the study aimed to evaluate the cytotoxicity of ozone for human gingival fibroblasts. Material and Methods: One hundred and eighty single-root teeth were contaminated with a mono-species biofilm of Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus. Groups were formed: Group I – control; Group II – standard protocol; Group III – standard protocol + ozone gas at 40 µg/mL; and Group IV – standard protocol + aqueous ozone at 8 µg/mL. In parallel, human gingival fibroblasts were submitted to the MTT test. Cells were plated, then ozone was applied as follows: Group I (control) – broth medium; Group II – aqueous ozone at 2 µg/mL; Group III – aqueous ozone at 5 µg/mL; and Group IV – aqueous ozone at 8 µg/mL. Data were submitted to the Kruskal Wallis test and Bonferroni post hoc analyses to assess microbiology and cytotoxicity, respectively (p<0.05%). Results The results revealed antimicrobial efficacy by Group IV with no CFU count. The cytotoxicity assay showed Groups III and IV to be the most aggressive, providing a decrease in cell viability at hour 0 from 100% to 77.3% and 68.6%, respectively. Such a decrease in cell viability was reverted, and after 72 hours Groups III and IV provided the greatest increase in cell viability, being statistically different from Groups I and II. Conclusion According to the applied methodology and the limitations of this study, it was possible to conclude that ozone therapy improved the decontamination of the root canal ex vivo. Ozone was toxic to the cells on first contact, but cell viability was recovered. Thus, these findings suggest that ozone might be useful to improve root canal results.

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OBJECTIVES: This study evaluated the antimicrobial efficacy of ozone therapy in teeth contaminated with Pseudomonas aeruginosa, Enterococcus faecalis, and Staphylococcus aureus using a mono-species biofilm model. Parallel to this, the study aimed to evaluate the cytotoxicity of ozone for human gingival fibroblasts. Material and Methods: One hundred and eighty single-root teeth were contaminated with a mono-species biofilm of Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus. Groups were formed: Group I - control; Group II - standard protocol; Group III - standard protocol + ozone gas at 40 µg/mL; and Group IV - standard protocol + aqueous ozone at 8 µg/mL. In parallel, human gingival fibroblasts were submitted to the MTT test. Cells were plated, then ozone was applied as follows: Group I (control) - broth medium; Group II - aqueous ozone at 2 µg/mL; Group III - aqueous ozone at 5 µg/mL; and Group IV - aqueous ozone at 8 µg/mL. Data were submitted to the Kruskal Wallis test and Bonferroni post hoc analyses to assess microbiology and cytotoxicity, respectively (p<0.05%). RESULTS: The results revealed antimicrobial efficacy by Group IV with no CFU count. The cytotoxicity assay showed Groups III and IV to be the most aggressive, providing a decrease in cell viability at hour 0 from 100% to 77.3% and 68.6%, respectively. Such a decrease in cell viability was reverted, and after 72 hours Groups III and IV provided the greatest increase in cell viability, being statistically different from Groups I and II. CONCLUSION: According to the applied methodology and the limitations of this study, it was possible to conclude that ozone therapy improved the decontamination of the root canal ex vivo. Ozone was toxic to the cells on first contact, but cell viability was recovered. Thus, these findings suggest that ozone might be useful to improve root canal results.

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Na era da colaboração, surge o Ensino a Distância (EaD) como poderosa ferramenta de divulgação de realidades, permitindo que a importância do BDH seja divulgada adequadamente. Diversas iniciativas estão sendotomadas de modo articulado, a fim de facilitar a criação e a consolidação de uma rede de colaborativa detrabalho em Telessaúde e Teleodontologia para a divulgação e implementação de Bancos de Dentes Humanosem sua faculdade ou centro de pesquisasbaseado no caminho trilhado pela Faculdade de Odontologia da USP (AU).

In the era of collaboration, arises Distance Education (DE) as a powerful tool for the dissemination of realities, allowing the importance of BDH is disclosed properly. Several initiatives are being taken in an articulate manner in order to facilitate the creation and consolidation of a network of collaborative working in Telehealth and Teledentistry for the dissemination and implementation of Banks Human Teeth at your college or research center based on the path followed by School of Dentistry, USP (AU).

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O Núcleo de Teleodontologia da Faculdade de Odontologia da Universidade de São Paulo (NTO-FOUSP) foi criado em articulação com o Núcleo São Paulo de Telessaúde, como parte do Programa Telessaúde Brasil, pelo Ministério da Saúde. Seu foco: dar suporte aos professores para uso das TIC. Também deverá oferecer teleconsultorias e Segundas Opiniões Formativas aos profissionais de saúde do Sistema Único de Saúde (SUS).

The Teledentistry Center at the Faculty of Dentistry (NTO-FOUSP), University of São Paulo, was created in articulation with the Center of Telehealth of São Paulo, as part of the Brazilian Telehealth Program, by the Ministry of Health. Its first aim was to offer support to the professors for a better use of ICT. It should also offer teleconsulting and the Second Formative Opinion for health professionals working at the Unified Health System (SUS).

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A ciência inovadora da Teleodontologia oferece conhecimento odontológico em dois âmbitos principais: a teleassistência e a teleducação. Em se tratando de inovações tecnológicas trazidas pelas Novas Tecnologias de Informação e Comunicação (NTICl, é possível recorrer a esses conhecimentos para aplicação em diversos âmbitos da vida profissional, como uma nova visão de clínica interdisciplinar ou maneiras criativas de se aplicar o marketing profissional. O texto ainda retrata os cuidados devidos à manutenção do sigilo das informações relativas aos pacientes e formas seguras e criativas de se incorporar as NTIC no dia a dia do Cirurgião-Dentista, acrescentando valor à ação profissional e qualificação na atenção à saúde bucal brasileira.

The innovative science of Teledentistry offers dental knowledge in two main areas: the teleducation and teleassistance. Technological innovations brought by the New Information and Communication Technologies (lCTs) offers this knowledge for application in various areas of professional life, as a new vision of interdisciplinary clinical and creative ways to apply in the marketing professional. The text also explores the care of the confidentiality maintenance of information relating to patients and safe and creative ways to incorporate ICT in the dentist day-by-day, adding value to the professional action and qualifying the oral health care in Brazil..

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This study compared the coronal and apical leakage of AH Plus with gutta-percha to that of Epiphany with Resilon. Twenty-four single rooted teeth were instrumented and divided into 2 groups according to the solutions for smear layer removal and the obturation materials employed: Group A - 17% EDTA-T and AH Plus with gutta-percha; Group B - primer and Epiphany with Resilon. The Group B specimens were light-cured in the coronal area for 20 s. The external root surfaces were covered with a double layer of ethyl cyanoacrylate, except for the apical foramen and the cavity access. The teeth were immersed in 0.5% methylene blue for 48 h. The specimens were rinsed, dried and axially split for dye penetration measurement with the ImageLab 2.3 software. The t-test showed no significant differences for coronal leakage between the groups, but there were significant differences for apical leakage between the groups (P < 0.05). AH Plus with gutta-percha and Epiphany with Resilon provided the same coronal seal, whereas Epiphany with Resilon provided the best apical seal.

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