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Episodic memory depends on the recollection of spatial and temporal aspects of past experiences in which the hippocampus plays a critical role. Studies on hippocampal lesions in rodents have shown that dentate gyrus (DG) and CA3 are necessary to detect object displacement in memory tasks. However, the understanding of real-time oscillatory activity underlying memory discrimination of subtle and pronounced displacements remains elusive. Here, we chronically implanted microelectrode arrays in adult male Wistar rats to record network oscillations from DG, CA3, and CA1 of the dorsal hippocampus while animals executed an object recognition task of high and low spatial displacement tests (HD: 108 cm, and LD: 54 cm, respectively). Behavioral analysis showed that the animals discriminate between stationary and displaced objects in the HD but not LD conditions. To investigate the hypothesis that theta and gamma oscillations in different areas of the hippocampus support discrimination processes in a recognition memory task, we compared epochs of object exploration between HD and LD conditions as well as displaced and stationary objects. We observed that object exploration epochs were accompanied by strong rhythmic activity in the theta frequency (6-12 Hz) band in the three hippocampal areas. Comparison between test conditions revealed higher theta band power and higher theta-gamma phase-amplitude coupling in the DG during HD than LD conditions. Similarly, direct comparison between displaced and stationary objects within the HD test showed higher theta band power in CA3 during exploration of displaced objects. Moreover, the discrimination index between displaced and stationary objects directly correlated with CA1 gamma band power in epochs of object exploration. We thus conclude that theta and gamma oscillations in the dorsal hippocampus support the successful discrimination of object displacement in a recognition memory task.

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ABSTRACT Objectives: to validate a scenario for clinical simulation: prenatal nursing consultation for adolescents. Methods: methodological study developed from January to December 2019, in five stages (overview, scenario, scenario design, progression, debriefing and assessment). The validation involved four volunteer students, a teacher as a facilitator and four judges. The judges filled out a Likert scale with four responses. Data was analyzed using Microsoft Excel® software, version 2016. Absolute and relative frequencies and the content validity index were calculated, considering a minimum acceptable value of 1.0. Results: the preparation of the scenario was based on the proposed learning objectives. The scenario was validated with a global content validity index equal to 1.0. Final Considerations: the study achieved the proposed objective. This scenario can contribute to preparing nurses to work in the care of pregnant teenagers, a representative public in Brazil that requires specific care.

RESUMEN Objetivos: validar un escenario para simulado clínico, en enseñanza de enfermería, sobre primera consulta de prenatal a gestante adolescente. Métodos: estudio metodológico desarrollado de enero a diciembre de 2019, en cinco etapas (overview, scenario, scenario design progression, debriefing y assessment). La validación involucró cuatro alumnos voluntarios, una docente como facilitadora y cuatro jueces. Los jueces rellenaron una escala Likert con cuatro respuestas. Datos fueran analizados en el software Microsoft Excel®, versión 2016. Calculadas las frecuencias absolutas y relativas y el índice de validez de contenido, considerando valor mínimo aceptable de 1,0. Resultados: la elaboración del escenario partió de los objetivos de aprendizaje propuestos. El escenario fue validado con índice de validez de contenido global igual a 1,0. Consideraciones Finales: el estudio alcanzó el objetivo propuesto. Este escenario puede contribuir para preparar enfermeros para actuación en la atención a gestantes adolescentes, un público representativo en Brasil y que requiere cuidados específicos.

RESUMO Objetivos: validar um cenário para simulação clínica, no ensino de enfermagem, sobre primeira consulta de pré-natal à gestante adolescente. Métodos: estudo metodológico desenvolvido de janeiro a dezembro de 2019, em cinco etapas (overview, scenario, scenario design progression, debriefing e assessment). A validação envolveu quatro alunos voluntários, uma docente como facilitadora e quatro juízes. Os juízes preencheram uma escala Likert com quatro respostas. Os dados foram analisados no software Microsoft Excel®, versão 2016. Calcularam-se as frequências absolutas e relativas e o índice de validade de conteúdo, considerando valor mínimo aceitável de 1,0. Resultados: a elaboração do cenário partiu dos objetivos de aprendizagem propostos. O cenário foi validado com índice de validade de conteúdo global igual a 1,0. Considerações Finais: o estudo alcançou o objetivo proposto. Este cenário pode contribuir para preparar enfermeiros para atuação na atenção às gestantes adolescentes, um público representativo no Brasil e que requer cuidados específicos.

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OBJECTIVES: to validate a scenario for clinical simulation: prenatal nursing consultation for adolescents. METHODS: methodological study developed from January to December 2019, in five stages (overview, scenario, scenario design, progression, debriefing and assessment). The validation involved four volunteer students, a teacher as a facilitator and four judges. The judges filled out a Likert scale with four responses. Data was analyzed using Microsoft Excel® software, version 2016. Absolute and relative frequencies and the content validity index were calculated, considering a minimum acceptable value of 1.0. RESULTS: the preparation of the scenario was based on the proposed learning objectives. The scenario was validated with a global content validity index equal to 1.0. FINAL CONSIDERATIONS: the study achieved the proposed objective. This scenario can contribute to preparing nurses to work in the care of pregnant teenagers, a representative public in Brazil that requires specific care.

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The purpose of this paper is to propose a framework for cybersecurity risk management in telemedicine. The framework, which uses a bow-tie approach for medical image diagnosis sharing, allows the identification, analysis, and assessment of risks, considering the ISO/TS 13131:2014 recommendations. The bow-tie method combines fault tree analysis (FTA) and event tree analysis (ETA). The literature review supported the identification of the main causes and forms of control associated with cybersecurity risks in telemedicine. The main finding of this paper is that it is possible, through a structured model, to manage risks and avoid losses for everyone involved in the process of exchanging medical image information through telemedicine services. Through the framework, those responsible for the telemedicine services can identify potential risks in cybersecurity and act preventively, recognizing the causes even as, in a mitigating way, identifying viable controls and prioritizing investments. Despite the existence of many studies on cybersecurity, the paper provides theoretical contributions to studies on cybersecurity risks and features a new methodological approach, which incorporates both causes and consequences of the incident scenario.

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BACKGROUND: Episodic-like memory tasks based on the spontaneous exploration of objects are commonly applied in one-trial protocols. However, multiple-trial designs are known to reduce animal numbers and data variance, providing faster accumulation of data. NEW METHOD: In this study, we devised a new object recognition memory task for rats that carry out multiple trials per session. We developed three types of continual trial tasks: a longer protocol, a shorter protocol, and a protocol in which the experimental session was divided into two days. RESULTS: In our design, rats expressed temporal and spatial memory, but not what-where-when content integration. We found that shorter protocols were more efficient to evaluate memory capabilities. COMPARISON WITH EXISTING METHODS: To the best of our knowledge, it is the first object recognition task with multiple trials that simultaneously assess the temporal and spatial aspects of episodic-like memory. CONCLUSIONS: We suggest that our task is suitable for the simultaneous measurements of brain functions related to spatial and temporal attributes in rats.

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The aim of the present study was to evaluate the construct validity, internal consistency and psychometric indicators of the reduced version of the Cognitive Emotion Regulation Questionnaire (CERQ-short). The CERQ-short was adapted and translated to Portuguese prior to its administration to 254 adults, mainly from the state of Paraíba, Brazil. The Difficulties in Emotion Regulation Scale (DERS), the Compulsive Buying Scale (CBS) and a sociodemographic questionnaire were also applied. The original CERQ-short nine-factor structure was preserved. The convergent and discriminant validity, the reliability and the psychometric adequacy concerning item difficulty and item discrimination were also confirmed. The results support the use of this scale for rapid screening and research. (AU)

O presente estudo teve como objetivo buscar evidências de validade de construto, de consistência interna e indicadores psicométricos dos itens da versão adaptada para o português do Questionário Cognitivo de Regulação Emocional versão reduzida (CERQ-short). Após tradução e adaptação, foi aplicado o CERQ-Short, as escalas de dificuldade em regulação emocional (DERS) e de Compras Compulsivas (CBS), além de um questionário demográfico em uma amostra de 254 adultos, maioria de paraibanos. Verificou-se que a estrutura original de nove fatores do CERQ-Short foi mantida, também foram confirmadas as validades convergente e discriminante, a fidedignidade e a adequação psicométrica dos itens no que se refere à dificuldade e à discriminação dos itens. A escala apresentou evidência para a utilização em rastreio rápido e pesquisas. (AU)

El presente artículo tuvo como objetivo la búsqueda de evidencias de la validez del construto e indicadores psicométricos de los ítems, de una versión adaptada para el portugués, del Cuestionario Cognitivo de Regulación Emocional, versión reducida (CERQ-short). Inicialmente, fue realizada la traducción y adaptación semántica; posteriormente, fue aplicado el cuestionario CERQ-Short a las escalas de dificultad en regulación emocional (DERS) y de las Compras Compulsivas (CBS), además de un cuestionario demográfico en una muestra de 254 adultos, la mayoría de los paraibanos. Se verificó que la estructura original de nueve factores del CERQ-Short fue mantenida, así como las evidencias de validez del construto, fiabilidad y adecuación psicométrica de los ítems. La escala presentó evidencias para la utilización en rastreo rápido y pesquisas. (AU)

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Objetivo: analisar os efeitos da acupuntura nos desconfortos físicos e emocionais presentes na gestação. Método: estudo transversal realizado com 45 gestantes do segundo e terceiro trimestre gestacional, atendidas no pré-natal de risco habitual e que realizaram sessões de acupuntura. Cada gestante fazia duas sessões por semana com duração de 30 minutos cada uma. Resultados: evidenciou-se que, após seis sessões de acupuntura, utilizando os pontos sistêmicos B57, B40, VB30, B23, B60, VG20 e pontos de auriculoterapia: Shemen, lombar e ciático, houve uma significativa melhoria de diversas queixas comuns na gestação, relatadas pelas participantes, como: relaxamento do corpo (97,8%); mudança do estresse (82,2%); sono (86,7%); estado de humor (82,2%); paciência (80%); energia (77,8); ansiedade (66,7%), dentre outros. Conclusão: a acupuntura contribuiu positivamente na melhoria de sintomas físicos e emocionais referidos pelas gestantes, podendo ser usada como alternativa para a promoção da saúde gestacional

Objective: to analyze the effects of acupuncture on physical and emotional discomfort present during gestation. Method: cross-sectional study carried out with 45 pregnant women in the second and third gestational trimesters receiving prenatal care for pregnancy of normal risk who underwent acupuncture sessions. Each pregnant woman attended two sessions per week with a duration of 30 minutes each. Results: after six sessions of acupuncture using the B57, B40, VB30, B23, B60, VG20 systemic points and the Shemen, lumbar and sciatic auriculotherapy points, there was a significant improvement in several common gestational complaints reported by the participants, such as: body relaxation (97.8%); change in stress levels (82.2%); sleep (86.7%); mood (82.2%); patience (80%); energy (77.8); anxiety (66.7%), among others. Conclusion: acupuncture contributed positively to the improvement of the physical and emotional symptoms mentioned by the pregnant women and can be used as an alternative for the promotion of gestational health

Objetivo: analizar los efectos de la acupuntura en las molestias físicas y emocionales presentes en la gestación. Método: estudio transversal realizado con 45 gestantes del segundo y tercer trismestre gestacional, atendidas en el pre natal de riesgo habitual y que realizaron sesiones de acupuntura. Cada gestante hacía dos sesiones por semana con una duración de 30 minutos cada una. Resultados: en la mayoría de los casos, se observó que, después de seis sesiones de acupuntura, utilizando los puntos sistémicos B57, B40, VB30, B23, B60, VG20 y puntos de auriculoterapia: Shemen, lumbar y ciático, hubo una significativa mejora de diversas quejas comunes en la gestación, de las participantes, como: relajación del cuerpo (97,8%); cambio del estrés (82,2%); (86,7%); estado de humor (82,2%); (80%); energía (77,8); la ansiedad (66,7%), entre otros. Conclusión: la acupuntura contribuyó positivamente en la mejora de los síntomas físicos y emocionales referidos por las gestantes, pudiendo ser usada como alternativa para la promoción de la salud gestacional

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The organophosphate temephos has been the main insecticide used against larvae of the dengue and yellow fever mosquito (Aedes aegypti) in Brazil since the mid-1980s. Reports of resistance date back to 1995; however, no systematic reports of widespread temephos resistance have occurred to date. As resistance investigation is paramount for strategic decision-making by health officials, our objective here was to investigate the spatial and temporal spread of temephos resistance in Ae. aegypti in Brazil for the last 12 years using discriminating temephos concentrations and the bioassay protocols of the World Health Organization. The mortality results obtained were subjected to spatial analysis for distance interpolation using semi-variance models to generate maps that depict the spread of temephos resistance in Brazil since 1999. The problem has been expanding. Since 2002-2003, approximately half the country has exhibited mosquito populations resistant to temephos. The frequency of temephos resistance and, likely, control failures, which start when the insecticide mortality level drops below 80%, has increased even further since 2004. Few parts of Brazil are able to achieve the target 80% efficacy threshold by 2010/2011, resulting in a significant risk of control failure by temephos in most of the country. The widespread resistance to temephos in Brazilian Ae. aegypti populations greatly compromise effective mosquito control efforts using this insecticide and indicates the urgent need to identify alternative insecticides aided by the preventive elimination of potential mosquito breeding sites.

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The organophosphate temephos has been the main insecticide used against larvae of the dengue and yellow fever mosquito (Aedes aegypti) in Brazil since the mid-1980s. Reports of resistance date back to 1995; however, no systematic reports of widespread temephos resistance have occurred to date. As resistance investigation is paramount for strategic decision-making by health officials, our objective here was to investigate the spatial and temporal spread of temephos resistance in Ae. aegypti in Brazil for the last 12 years using discriminating temephos concentrations and the bioassay protocols of the World Health Organization. The mortality results obtained were subjected to spatial analysis for distance interpolation using semi-variance models to generate maps that depict the spread of temephos resistance in Brazil since 1999. The problem has been expanding. Since 2002-2003, approximately half the country has exhibited mosquito populations resistant to temephos. The frequency of temephos resistance and, likely, control failures, which start when the insecticide mortality level drops below 80%, has increased even further since 2004. Few parts of Brazil are able to achieve the target 80% efficacy threshold by 2010/2011, resulting in a significant risk of control failure by temephos in most of the country. The widespread resistance to temephos in Brazilian Ae. aegypti populations greatly compromise effective mosquito control efforts using this insecticide and indicates the urgent need to identify alternative insecticides aided by the preventive elimination of potential mosquito breeding sites.

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A resistência de populações de Aedes aegypti a inseticidas químicos tem representado um desafio nos programas para seu controle. Este projeto teve como objetivos caracterizar o perfil de susceptibilidade de populações de A. aegypti de Pernambuco, relacionando-o ao histórico local de uso de tais compostos e aos mecanismos que podem estar associados à resistência. Amostras de A. aegypti de 17 municípios foram analisadas através de bioensaios com o temephos (larvicida) e diflubenzuron (regulador de crescimento) e um adulticida, a cipermetrina. Testes bioquímicos foram realizados para quantificar a atividade das enzimas acetilcolinesterase (ACE), glutationa S-transferase (GST), esterases (Alfa, Beta e PNPA) e oxidases de função mista (MFO). Também foram investigadas mutações no gene do canal de sódio: sítios 982, 1011, 1014, e 1016. Os resultados demonstraram que todas as populações estavam resistentes ao temephos, exceto a de Fernando de Noronha. A razão de resistência (RR) foi moderada apenas na população Recife, enquanto RR 100 vezes foram observadas em 10 populações. Houve uma correlação entre o consumo e a RR a este produto. Para o diflubenzuron foi construída uma linha de base dose resposta e as RR foram correlacionadas positivamente com as observadas para o temephos...

Resistance to chemical insecticides represents a challenge for Aedes aegypticontrol programs. This project aimed to characterize the susceptibility status ofA. aegypti populations fromPernambuco state, associating it to the local use history...

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