RESUMO
Aclacinomycin (ACM) is an oncostatic of the anthracycline family, largely used in patients and experimentally in mice. ACM has been reported to enhance phagocytosis, secretion of free oxygen radicals and of interleukin 1. Its injection is also followed by an increase of the cytotoxic and cytostatic activity of murine peritoneal macrophages. In the present work we investigated whether ACM modifies the antigen-presenting cell capacity of murine peritoneal macrophages. Purified T lymphocytes were cultured with peritoneal macrophages from either normal or ACM treated mice (4 mg/kg day -4) which were previously incubated with phytohemagglutinin. The T cell proliferative response was greater in cultures with normal macrophages, indicating that macrophages from ACM-treated mice had a better antigen presenting activity than normal untreated macrophages.
Assuntos
Aclarubicina/farmacologia , Antibióticos Antineoplásicos/farmacologia , Células Apresentadoras de Antígenos/imunologia , Macrófagos Peritoneais/imunologia , Animais , Imunidade Celular/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos BALB CRESUMO
Female, 5 years old; first pregnancy of term, eutocic, weight 3,100 g; breast fed, complete vaccination program. A bronchial pathology that was present seven months later, yielded with medical treatment. Her illness started on September, 1984 after sever trauma by horse kick, presenting with a tumor in left illiac fossa; there was pain, improved by analgesics; the tumor continued to grow up to 5 x 6 cm in diameter, painful on palpation, causing hospital admission. Thirty days later an ultrasonographic study reported an ovoid echogenic and echolucid mass of 6.5 cm in left ovary; an infra-umbilical laparotomy was performed, finding a left ovarian tumarration, ovoid, pink white, with vascular areas, 24 x 18 x 15 cm at maximal diameters, solid, smooth and bright; at section there was gray-white, nodular tissue, with cystic cavities, 0.5 cm. Electronic microscopy an immunochemistry study were carried out in order to confirm the diagnosis of juvenile granulosa cell tumor. The patient died seven months later.
Assuntos
Tumor de Células da Granulosa/ultraestrutura , Neoplasias Ovarianas/ultraestrutura , Pré-Escolar , Feminino , HumanosRESUMO
In 1977 Scully first described the juvenile granulosa cell tumor of the ovary (JGCT) as a special variation of the granulosa cells tumor, which occurs in the two first decades of life, and 97% of the cases show characteristic microscopic and histological features. Five previous cases have been reported concerning the ultramicroscopic characteristics of this ovarian neoplasia. The purpose of this paper is to report the immunohistochemical and ultrastructural characteristics in a case of OJCGT which occurred in a four year old girl with isosexual precocious pseudopuberty. The presence of vimentin and absence of keratin was proven immunohistochemically in this ovarian neoplasia. Intermediate filaments were found ultrastructurally. The combined use of immunohistochemical and ultrastructural techniques has proven to be of extraordinary usefulness for the differential diagnosis between epithelial and non epithelial ovarian tumors and adds a new and highly specific method to characterize and differentiate the cells of embryonic carcinoma, choriocarcinomas and endodermal sinus tumors which are keratin positive.