RESUMO

Banana 'Prata' has a significant demand in the Brazilian market, and Minas Gerais is one of the largest banana producers in the country. Scientific studies that evaluate the bananas quality produced in different cultivation regions are still incipient. Thus, this study evaluated the physical, biochemical and sensory attributes of banana 'Prata' from south and north of Minas Gerais and Vale do Ribeira (SP). Bananas from south were also cultivated in different production systems, organic and conventional. Data were submitted to multivariate analysis that provided the discrimination of the samples according to the cultivation regions. Bananas from southern presented higher levels of soluble solids and acidity, better taste, higher diameter and overall acceptance, where in the organic bananas were the most preferred by consumers. Fruit from northern stood out in appearance, texture, aroma and color, in addition to greater length. Fruit from Vale do Ribeira had higher levels of total phenolics and antioxidant activity. With these results we can assume that fruit quality is highly related to the specific climatic conditions from each producing region. Bananas from the south of Minas Gerais showed superiority for most of the evaluated traits, reflecting on consumer preference.

Assuntos

Musa , Frutas , Paladar , Odorantes , Antioxidantes

RESUMO

Capsicum annuum is one of the most important horticultural crops worldwide. Anthracnose disease (Colletotrichum spp.) is a major constraint for chili production, causing substantial losses. Capsidiol is a sesquiterpene phytoalexin present in pepper fruits that can enhance plant resistance. The genetic mechanisms involved in capisidiol biosynthesis are still poorly understood. In this study, a 3' RNA sequencing approach was used to develop the transcriptional profile dataset of C. annuum genes in unripe (UF) and ripe fruits (RF) in response to C. scovillei infection. Results showed 4,845 upregulated and 4,720 downregulated genes in UF, and 2,560 upregulated and 1,762 downregulated genes in RF under fungus inoculation. Four capsidiol-related genes were selected for RT-qPCR analysis, two 5-epi-aristolochene synthase (CA12g05030, CA02g09520) and two 5-epi-aristolochene-1,3-dihydroxylase genes (CA12g05070, CA01g05990). CA12g05030 and CA01g05990 genes showed an early response to fungus infection in RF (24 h post-inoculation-HPI), being 68-fold and 53-fold more expressed at 96 HPI, respectively. In UF, all genes showed a late response, especially CA12g05030, which was 700-fold more expressed at 96 HPI compared to control plants. We are proving here the first high-throughput expression dataset of pepper fruits in response to anthracnose disease in order to contribute for future pepper breeding programs.

Assuntos

Capsicum/genética , Capsicum/microbiologia , Colletotrichum , Frutas/genética , Regulação da Expressão Gênica de Plantas , Desenvolvimento Vegetal/genética , Sesquiterpenos/metabolismo , Biologia Computacional/métodos , Mineração de Dados , Frutas/metabolismo , Perfilação da Expressão Gênica , Doenças das Plantas/genética , Doenças das Plantas/microbiologia , Transcriptoma

RESUMO

Coffea arabica L. is an important agricultural commodity, accounting for 60% of traded coffee worldwide. Nitrogen (N) is a macronutrient that is usually limiting to plant yield; however, molecular mechanisms of plant acclimation to N limitation remain largely unknown in tropical woody crops. In this study, we investigated the transcriptome of coffee roots under N starvation, analyzing poly-A+ libraries and small RNAs. We also evaluated the concentration of selected amino acids and N-source preferences in roots. Ammonium was preferentially taken up over nitrate, and asparagine and glutamate were the most abundant amino acids observed in coffee roots. We obtained 34,654 assembled contigs by mRNA sequencing, and validated the transcriptional profile of 12 genes by RT-qPCR. Illumina small RNA sequencing yielded 8,524,332 non-redundant reads, resulting in the identification of 86 microRNA families targeting 253 genes. The transcriptional pattern of eight miRNA families was also validated. To our knowledge, this is the first catalog of differentially regulated amino acids, N sources, mRNAs, and sRNAs in Arabica coffee roots.

Assuntos

Coffea/genética , MicroRNAs/genética , Nitrogênio/deficiência , RNA Mensageiro/genética , RNA de Plantas/genética , Pequeno RNA não Traduzido/genética , Aminoácidos/isolamento & purificação , Aminoácidos/metabolismo , Compostos de Amônio/metabolismo , Coffea/metabolismo , Regulação da Expressão Gênica de Plantas , Ontologia Genética , Sequenciamento de Nucleotídeos em Larga Escala , MicroRNAs/classificação , MicroRNAs/metabolismo , Anotação de Sequência Molecular , Nitratos/metabolismo , Folhas de Planta/genética , Folhas de Planta/metabolismo , Raízes de Plantas/genética , Raízes de Plantas/metabolismo , Poli A/genética , Poli A/metabolismo , RNA Mensageiro/classificação , RNA Mensageiro/metabolismo , RNA de Plantas/classificação , RNA de Plantas/metabolismo , Pequeno RNA não Traduzido/classificação , Pequeno RNA não Traduzido/metabolismo , Sementes/genética , Sementes/metabolismo , Estresse Fisiológico , Transcriptoma

RESUMO

Coffea arabica L. is an important agricultural commodity, accounting for 60% of traded coffee worldwide. Nitrogen (N) is a macronutrient that is usually limiting to plant yield; however, molecular mechanisms of plant acclimation to N limitation remain largely unknown in tropical woody crops. In this study, we investigated the transcriptome of coffee roots under N starvation, analyzing poly-A+ libraries and small RNAs. We also evaluated the concentration of selected amino acids and N-source preferences in roots. Ammonium was preferentially taken up over nitrate, and asparagine and glutamate were the most abundant amino acids observed in coffee roots. We obtained 34,654 assembled contigs by mRNA sequencing, and validated the transcriptional profile of 12 genes by RT-qPCR. Illumina small RNA sequencing yielded 8,524,332 non-redundant reads, resulting in the identification of 86 microRNA families targeting 253 genes. The transcriptional pattern of eight miRNA families was also validated. To our knowledge, this is the first catalog of differentially regulated amino acids, N sources, mRNAs, and sRNAs in Arabica coffee roots.