RESUMO

Three different hybridoma clones secreting anti-HBsAg antibody were constructed by fusing cells of mouse myeloma line Ag8-X63 with splenocytes from BALB/c mice immunized with recombinant HBsAg and natural HBV. The monoclonal antibodies obtained were characterized immunologically, and two were used to develop UMELISA for detection of HBsAg. This monoclonal assay enabled the detection of 0.1 UPE/mL with reference to the standard of the Paul Ehrlich Institute (Frankfurt, Germany). The assay compared well with a commercially available kit (UMELISA HBsAg) and was used for detection of HBsAg in blood donors.

Assuntos

Anticorpos Monoclonais/química , Antígenos de Superfície da Hepatite B/imunologia , Hepatite B/diagnóstico , Hepatite B/imunologia , Animais , Feminino , Antígenos de Superfície da Hepatite B/sangue , Humanos , Hibridomas , Camundongos , Camundongos Endogâmicos BALB C