RESUMO
The objective of this study was to evaluate the environmental effects on embryo recovery rate and pregnancy rate of Mangalarga Marchador mares. The reproductive characteristics of donor and recipient mares were evaluated during five years in Brazilian tropical environment. The mares were used throughout the year and seasons were classified as: October to April (breeding season - BS); May (autumn transition out of the breeding season - ATBS); June to August (non-breeding season - nBS); and September (vernal transition into the breeding season - VTBS). Daily temperature rainfall and hours of daylight (photoperiod) were measured during all months and years of evaluation. The embryo recovery rate (ERR) and the pregnancy rate (PR) were observed and frequencies were calculated. The effect of environmental variables, day of flushing, and hormonal treatments (estradiol benzoate and progesterone) were determined for the reproductive measures using the Chi-square and Kruskal-Wallis tests. Significant effects were noted of the year, season and temperature on ERR (Pâ¯<â¯0.05), but no significant effects were observed of the environmental parameters (year, season, hormone treatment, rainfall and photoperiod) on PR (Pâ¯>â¯0.05). The day of uterine flush affected ERR (Pâ¯<â¯0.05) but did not affect PR (Pâ¯>â¯0.05). In addition, hormone treatment also supported favorable results of PR in recipient mares during nBS. The conclusion is that mares of this breed can be used in reproduction all year long, with good pregnancy rates, in Brazil's tropical environment. The hormone treatment also supported favorable pregnancy rates in recipient mares during the non-breeding season. It seems that mares can have good pregnancy rates throughout the year in Brazil´s tropical environment.
Assuntos
Transferência Embrionária , Meio Ambiente , Cavalos/fisiologia , Animais , Brasil , Anticoncepcionais/administração & dosagem , Anticoncepcionais/farmacologia , Estradiol/administração & dosagem , Estradiol/análogos & derivados , Estradiol/farmacologia , Feminino , Ovulação/efeitos dos fármacos , Ovulação/fisiologia , Gravidez , Taxa de Gravidez , Progesterona/administração & dosagem , Progesterona/farmacologia , Doadores de Tecidos , Coleta de Tecidos e ÓrgãosRESUMO
The aim of the present study was to verify the effect of salmon oil addition on cryopreservation of equine semen. The experiment consisted of two treatments. Treatment 1 (T1) (control diluent), BotuCrio® was used without addition of salmon oil and treatment 2 (T2) (experimental diluent) BotuCrio® plus (with) 2% salmon oil. Three ejaculates of four stallions were used, totalizing 12 collections (n=12). Overall motility and progressive motility were evaluated by the Hamilton Thorn Research (HTR) Ceros 10.8 program, as well as the plasma membrane functionality through the hyposmotic test. Both treatments did not present statistical differences in relation to motility (T1 25,2±1,7 a, T2 29,7±1,9 a) and progressive motility (T1 11,0±1,1 a, T2 14,1±1,3 a). With respect to the hyposmotic test, the treatment 2 plus 2% of Salmon oil, presented better protection of sperm membrane functionality in relation to the control treatment (T2 77,3±1,0 a, T1 68,0±1,0 b). It can be concluded that salmon oil, although not altering the total and progressive motility, confers a better efficiency of sperm membrane functionality after thawing in equine semen.(AU)
O objetivo com este trabalho foi verificar o efeito da adição do óleo de salmão no congelamento do sêmen equino. O experimento constituiu de dois tratamentos. O tratamento 1 (T1) (diluente controle), utilizou-se o BotuCrio® sem a adição de óleo de salmão e o tratamento 2 (T2) (diluente experimental) BotuCrio® adicionado de 2% de óleo de salmão. Foram utilizados três ejaculados de quatro garanhões, totalizando doze coletas (n=12). Avaliou-se a motilidade total e motilidade progressiva pelo programa Ceros 10.8 da Hamilton Thorn Research (HTR), além da funcionalidade de membrana plasmática através do teste hiposmótico. Ambos os tratamentos não apresentaram diferenças estatísticas em relação a motilidade total (T1 25,2±1,7 a, T2 29,7±1,9 a) e motilidade progressiva (T1 11,0±1,1 a, T2 14,1±1,3 a). Com relação ao teste hiposmótico, o tratamento 2 acrescido de 2 % de óleo de Salmão, apresentou melhor proteção da funcionalidade de membrana espermática em relação ao tratamento controle (T2 77,3±1,0 a, T1 68,0±1,0 b). Pode-se concluir que o óleo de salmão, apesar de não alterar a motilidade total e progressiva, confere uma melhor eficiencia da funcionalidade de membrana espermática pós descongelamento em sêmen de equinos.(AU)
Assuntos
Animais , Óleos de Peixe/administração & dosagem , Óleos de Peixe/análise , Preservação do Sêmen/métodos , Preservação do Sêmen/veterinária , Cavalos/embriologia , Criopreservação/veterináriaRESUMO
The aim of the present study was to verify the effect of salmon oil addition on cryopreservation of equine semen. The experiment consisted of two treatments. Treatment 1 (T1) (control diluent), BotuCrio® was used without addition of salmon oil and treatment 2 (T2) (experimental diluent) BotuCrio® plus (with) 2% salmon oil. Three ejaculates of four stallions were used, totalizing 12 collections (n=12). Overall motility and progressive motility were evaluated by the Hamilton Thorn Research (HTR) Ceros 10.8 program, as well as the plasma membrane functionality through the hyposmotic test. Both treatments did not present statistical differences in relation to motility (T1 25,2±1,7 a, T2 29,7±1,9 a) and progressive motility (T1 11,0±1,1 a, T2 14,1±1,3 a). With respect to the hyposmotic test, the treatment 2 plus 2% of Salmon oil, presented better protection of sperm membrane functionality in relation to the control treatment (T2 77,3±1,0 a, T1 68,0±1,0 b). It can be concluded that salmon oil, although not altering the total and progressive motility, confers a better efficiency of sperm membrane functionality after thawing in equine semen.
O objetivo com este trabalho foi verificar o efeito da adição do óleo de salmão no congelamento do sêmen equino. O experimento constituiu de dois tratamentos. O tratamento 1 (T1) (diluente controle), utilizou-se o BotuCrio® sem a adição de óleo de salmão e o tratamento 2 (T2) (diluente experimental) BotuCrio® adicionado de 2% de óleo de salmão. Foram utilizados três ejaculados de quatro garanhões, totalizando doze coletas (n=12). Avaliou-se a motilidade total e motilidade progressiva pelo programa Ceros 10.8 da Hamilton Thorn Research (HTR), além da funcionalidade de membrana plasmática através do teste hiposmótico. Ambos os tratamentos não apresentaram diferenças estatísticas em relação a motilidade total (T1 25,2±1,7 a, T2 29,7±1,9 a) e motilidade progressiva (T1 11,0±1,1 a, T2 14,1±1,3 a). Com relação ao teste hiposmótico, o tratamento 2 acrescido de 2 % de óleo de Salmão, apresentou melhor proteção da funcionalidade de membrana espermática em relação ao tratamento controle (T2 77,3±1,0 a, T1 68,0±1,0 b). Pode-se concluir que o óleo de salmão, apesar de não alterar a motilidade total e progressiva, confere uma melhor eficiencia da funcionalidade de membrana espermática pós descongelamento em sêmen de equinos.