RESUMO

Hemotherapy using whole blood and its components is being increasingly used in veterinary therapy. Since it is important to store animal blood while maintaining acceptable hematological, blood gas, and biochemical characteristics, increasing our knowledge of available technologies for strategic blood storage is imperative. Thus, we aimed to assess the hematological, blood gas, and biochemical changes in donkey whole blood using blood bags with two different types of storage agents. Eight adult healthy male donkeys were used; 900 mL of blood was collected from each, with 450 mL stored in citrate-phosphate-dextrose and adenine bags (CPDA-1) and 450 mL stored in bags containing citrate-phosphate-dextrose, adenine, mannitol, and sodium chloride (CPD/SAG-M). Both bags were kept refrigerated between 1 and 6 °C for 42 days. Blood samples were removed from the bags eight times (T): T0 (immediately after blood collection), T1, T3, T7, T14, T21, T35, and T42 (1, 3, 7, 14, 21, 35 and 42 days after storage). Hematological, blood gas, biochemical, and microbiological parameters were assessed. The CPDA-1 bags had a higher packed cell volume when compared to CPD/ SAG-M. The red blood cell count reduced by around 19% in both the bags due to hemolysis, which was confirmed by an increase in plasma hemoglobin. The white blood cell count; pH; concentrations of glucose, sodium, bicarbonate, and 2,3 diphosphoglycerate were reduced in both bags. Meanwhile, pO2, pCO2, lactate dehydrogenase, and levels of potassium increased in the CPDA-1 and CPD/SAG-M bags. Blood bags were efficient for the storage of donkey blood for up to 42 days.

RESUMO

BACKGROUND: This study aimed to evaluate the clinical, haematological and biochemical responses to autologous blood transfusion and the feasibility of this practice in sheep. Thus, we used eight male, 8 months old sheep, weighing on average 30 kg, from which 15 mL/kg of whole blood was collected and stored in CPDA-1 bags. Blood samples were refrigerated for 8 days and subsequently re-infused. The clinical, haematological and biochemical parameters were evaluated before blood collection and reinfusion, after 10 minutes of collection and reinfusion, after 3, 6, 12, 24, 48, 96 and 192 hours after collection and reinfusion. RESULTS: With respect to clinical parameters, we observed a decrease in heart rate after 24, 48 and 196 hours from reinfusion compared to basal values (p < 0.05). Haematological variables including globular volume and erythrocyte counts showed a significant decrease (p < 0.01) at all time points after collection and increased (p < 0.01) at all time points after reinfusion. There was a significant increase in total protein and calcium at all time points after reinfusion (p < 0.05). CONCLUSION: Autologous transfusion in sheep slightly altered the physiological, biochemical and haematological responses of sheep, indicating that the technique proposed is safe and can be applied in the clinical practice of this species. The 8 d period was not sufficient for complete recovery of the haematological parameters after blood collection.

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RESUMO

A brucelose é uma zoonose infecto-contagiosa causada por bactérias do gênero Brucella. Ela já foi diagnosticada em todos os Estados da federação. Porém, pouco se sabe em relação a sua prevalência nos rebanhos paraenses. Deste modo, o presente estudo objetiva determinar a ocorrência de animais soro reagentes à brucelose bovina, através de um estudo retrospectivo de resultados de exames realizados por laboratórios particulares em diferentes regiões do Estado. Foram selecionados três laboratórios de diagnóstico de brucelose localizados no nos municípios de Santarém, Novo Repartimento e Tomé-Açu. Os exames foram realizados em animais com idade superior a 24 meses. Obtiveram-se um total de 7.724 resultados de exames provenientes de 14 municípios. Para o diagnóstico da brucelose foi realizado o Teste de soro aglutinação com Antígeno Acidificado Tamponado (AAT). Os fatores de risco município, macrorregião, microrregião, categoria animal e padrão racial foram avaliados pelo teste do qui quadrado de Pearson, já os fatores sexo e histórico de vacinação do rebanho através do teste exato de Fisher, utilizando software estatístico Minitab. Foram observados 792 bovinos positivos, prevalência média de 10,25%. Em relação aos fatores de risco estudados, a prevalência foi maior em vacas e novilhas, quando comparadas aos touros, sendo conseqüentemente maior nas fêmeas em relação aos machos. Estes resultados indicam que a Brucelose está disseminada em todo o Estado do Pará, sendo observada uma alta prevalência desta enfermidade. Em vista dos resultados obtidos torna-se necessária a sensibilização de proprietários e profissionais quanto à importância do controle desta enfermidade.

Brucellosis is a zoonotic infectious disease caused by Brucella sp. Bovine brucellosis is present in over 128 countries. It is considered an endemic disease in Brazil and was diagnosed in all states. In relation to brucellosis, little is known about their prevalence in cattle herds from Pará state, Brazil. Thus, this study aims to determine the occurrence of cattle serum reagent to brucellosis by a retrospective study of the results of serological tests from private laboratories in different regions of from Pará state, Brazil. We selected three laboratories for the diagnosis of brucellosis located in the municipalities of Santarém, Novo Repartimento and Tomé-Açu. The animals tested for brucellosis were all older than 24 months. We obtained a total of 7,724 test results from 14 municipalities. For the diagnosis of brucellosis it was performed agglutination test with buffered acidified antigen. Qui-square test was performed to assess the possible risk factors for brucellosis prevalence in relation to macro and micro region, animal category and breed, and Fisher's exact test was applied for the factors sex and vaccination history of the herd. We observed 792 positive cattle, resulting in an average prevalence of 10.25%. The prevalence was higher in cows and heifers when compared to bulls, and consequently higher in females compared to males. Herds vaccinated against brucellosis showed lower prevalence in relation to properties without vaccination. These results indicate that brucellosis is widespread throughout the state of Pará, with a high prevalence of this disease. This results show the importance of convinces the cattle farmers and veterinaries of the importance of control this disease in the state.

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