RESUMO
The objective of this study was to evaluate the occurrence of anti-Leptospira spp. antibodies in female buffalo in the state of Pernambuco. A total of 123 female buffalo blood samples were collected from five properties distributed in the state of Pernambuco. The microscopic agglutination test was used to study anti-Leptospira spp. antibodies. The occurrence of anti-Leptospira spp. antibodies was 28.5% (35/123; CI 20.737.3%) and on different properties, the occurrence ranged from 28.6% to 80.0%, with 100% of the properties showing animals with positive results. The serovars of the serogroup Sejroe with a higher incidence were Hardjoprajtino (CTG strain, 49.1%) and Hardjo (Prajtino genotype, 43.2%), followed by serogroup Grippotyphosa with the Grippotyphosa serovar (3.9%), serogroup Pomona with the Pomona serovar (1.9%), and the Icterohaemorrhagiae serovar Copenhageni (1.9%). This was the first record of the occurrence of anti-Lepstospira spp. antibodies in female buffalo in the state of Pernambuco. Control measures are necessary to prevent health and economic losses, given that the agent involved affects animal reproduction, triggering drops in conception rates or even clinical cases of abortion.(AU)
Assuntos
Animais , Feminino , Bovinos , Búfalos/virologia , Leptospirose/epidemiologia , Leptospirose/veterinária , Anticorpos Antivirais , Leptospirose/imunologia , Leptospira/isolamento & purificação , Testes Sorológicos/veterinária , Brasil/epidemiologiaRESUMO
ABSTRACT The objective of this study was to evaluate the occurrence of anti-Leptospira spp. antibodies in female buffalo in the state of Pernambuco. A total of 123 female buffalo blood samples were collected from five properties distributed in the state of Pernambuco. The microscopic agglutination test was used to study anti-Leptospira spp. antibodies. The occurrence of anti-Leptospira spp. antibodies was 28.5% (35/123; CI 20.7-37.3%) and on different properties, the occurrence ranged from 28.6% to 80.0%, with 100% of the properties showing animals with positive results. The serovars of the serogroup Sejroe with a higher incidence were Hardjoprajtino (CTG strain, 49.1%) and Hardjo (Prajtino genotype, 43.2%), followed by serogroup Grippotyphosa with the Grippotyphosa serovar (3.9%), serogroup Pomona with the Pomona serovar (1.9%), and the Icterohaemorrhagiae serovar Copenhageni (1.9%). This was the first record of the occurrence of anti-Lepstospira spp. antibodies in female buffalo in the state of Pernambuco. Control measures are necessary to prevent health and economic losses, given that the agent involved affects animal reproduction, triggering drops in conception rates or even clinical cases of abortion.
Assuntos
Animais , Feminino , Bovinos , Búfalos/microbiologia , Doenças dos Bovinos/sangue , Leptospira/imunologia , Leptospirose/veterinária , Anticorpos Antibacterianos/sangue , Brasil , Testes de Aglutinação , Búfalos/imunologia , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/microbiologia , Sorogrupo , Leptospira/isolamento & purificação , Leptospira/genética , Leptospirose/imunologia , Leptospirose/microbiologia , Leptospirose/sangue , Anticorpos Antibacterianos/imunologiaRESUMO
This study aimed to determine the prevalence and risk factors associated with Leishmania spp. and Trypanosoma cruzi infections in dogs presented at veterinary clinics in the city of João Pessoa, Paraíba state, northeastern Brazil. A total of 384 dogs from 34 veterinary clinics were sampled between April 2015 and May 2016. For serological diagnosis of canine visceral leishmaniasis and Chagas disease, the indirect fluorescent antibody test was used. Reactive sera were submitted to a confirmatory test using the S7 ELISA. The seroprevalence of Leishmania spp. was 2.8% (11/384), and that of T. cruzi was 1.5% (6/384). Two animals were positive for both parasites. The variable "access to the street" was identified as a risk factor for Leishmania spp. infection (OR = 4.81; 95% CI = 1.23-18.75). We concluded that a significant proportion of dogs presented at veterinary clinics in João Pessoa are seropositive for Leishmania spp. or T. cruzi infections, and we suggest that greater care be taken with dogs that have access to the street.(AU)
O objetivo deste estudo foi determinar a prevalência e os fatores de risco associados à infecção por Leishmania spp. e Trypanosoma cruzi em cães atendidos em clínicas veterinárias na cidade de João Pessoa, Estado da Paraíba, Nordeste do Brasil. Foram utilizadas 384 cães procedentes de atendimentos de 34 clínicas veterinárias no período de abril de 2015 a maio de 2016. Para diagnóstico sorológico da leishmaniose visceral canina (LVC) e doença de Chagas (DC) foi empregada a reação de imunofluorescência indireta (RIFI). Os soros reagentes para ambas as infecções foram submetidos a uma prova confirmatória utilizando o ELISA S7. A soroprevalência para Leishimania spp. foi de 2,8% (11/384), e para T.cruzi foi de 1,5% (6/384). Dois animais foram positivos para ambos os parasitas. A variável acesso à rua foi identificada como fator de risco para infecção por Leishmania spp. (OR= 4,81; IC 95% = 1,23 -18,75). Conclui-se que os cães atendidos em clínicas veterinárias de João Pessoa estão expostos às infecções por Leishmania spp. e T. cruzi, bem como sugere-se maiores cuidados com cães que tem acesso à rua.(AU)
Assuntos
Animais , Cães , Cães/parasitologia , Leishmaniose Visceral/veterinária , Doença de Chagas/veterinária , Leishmania , Trypanosoma , Estudos SoroepidemiológicosRESUMO
The objective of this study was to evaluate the occurrence of anti-Leptospira spp. antibodies in female buffalo in the state of Pernambuco. A total of 123 female buffalo blood samples were collected from five properties distributed in the state of Pernambuco. The microscopic agglutination test was used to study anti-Leptospira spp. antibodies. The occurrence of anti-Leptospira spp. antibodies was 28.5% (35/123; CI 20.7-37.3%) and on different properties, the occurrence ranged from 28.6% to 80.0%, with 100% of the properties showing animals with positive results. The serovars of the serogroup Sejroe with a higher incidence were Hardjoprajtino (CTG strain, 49.1%) and Hardjo (Prajtino genotype, 43.2%), followed by serogroup Grippotyphosa with the Grippotyphosa serovar (3.9%), serogroup Pomona with the Pomona serovar (1.9%), and the Icterohaemorrhagiae serovar Copenhageni (1.9%). This was the first record of the occurrence of anti-Lepstospira spp. antibodies in female buffalo in the state of Pernambuco. Control measures are necessary to prevent health and economic losses, given that the agent involved affects animal reproduction, triggering drops in conception rates or even clinical cases of abortion.
Assuntos
Anticorpos Antibacterianos/sangue , Búfalos/microbiologia , Doenças dos Bovinos/sangue , Leptospira/imunologia , Leptospirose/veterinária , Testes de Aglutinação , Animais , Anticorpos Antibacterianos/imunologia , Brasil , Búfalos/imunologia , Bovinos , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/microbiologia , Feminino , Leptospira/genética , Leptospira/isolamento & purificação , Leptospirose/sangue , Leptospirose/imunologia , Leptospirose/microbiologia , SorogrupoRESUMO
This study aimed to determine the prevalence and risk factors associated with Leishmania spp. and Trypanosoma cruzi infections in dogs presented at veterinary clinics in the city of João Pessoa, Paraíba state, northeastern Brazil. A total of 384 dogs from 34 veterinary clinics were sampled between April 2015 and May 2016. For serological diagnosis of canine visceral leishmaniasis and Chagas disease, the indirect fluorescent antibody test was used. Reactive sera were submitted to a confirmatory test using the S7 ELISA. The seroprevalence of Leishmania spp. was 2.8% (11/384), and that of T. cruzi was 1.5% (6/384). Two animals were positive for both parasites. The variable "access to the street" was identified as a risk factor for Leishmania spp. infection (OR = 4.81; 95% CI = 1.23-18.75). We concluded that a significant proportion of dogs presented at veterinary clinics in João Pessoa are seropositive for Leishmania spp. or T. cruzi infections, and we suggest that greater care be taken with dogs that have access to the street.
O objetivo deste estudo foi determinar a prevalência e os fatores de risco associados à infecção por Leishmania spp. e Trypanosoma cruzi em cães atendidos em clínicas veterinárias na cidade de João Pessoa, Estado da Paraíba, Nordeste do Brasil. Foram utilizadas 384 cães procedentes de atendimentos de 34 clínicas veterinárias no período de abril de 2015 a maio de 2016. Para diagnóstico sorológico da leishmaniose visceral canina (LVC) e doença de Chagas (DC) foi empregada a reação de imunofluorescência indireta (RIFI). Os soros reagentes para ambas as infecções foram submetidos a uma prova confirmatória utilizando o ELISA S7. A soroprevalência para Leishimania spp. foi de 2,8% (11/384), e para T.cruzi foi de 1,5% (6/384). Dois animais foram positivos para ambos os parasitas. A variável acesso à rua foi identificada como fator de risco para infecção por Leishmania spp. (OR= 4,81; IC 95% = 1,23 -18,75). Conclui-se que os cães atendidos em clínicas veterinárias de João Pessoa estão expostos às infecções por Leishmania spp. e T. cruzi, bem como sugere-se maiores cuidados com cães que tem acesso à rua.