RESUMO
Despite the marked reduction in the incidence of measles in Brazil, a measles epidemic occurred in this country in 1997. The measles cases observed during this epidemic began to reappear in large numbers in São Paulo, and spread to Rio de Janeiro and other Brazilian states. In the present study molecular biology techniques were used for the detection and genomic characterization of measles viruses from clinical samples such as urine and nasopharyngeal secretions collected in the states of Rio de Janeiro, Minas Gerais and Paraná, during the 1997 epidemic. RT-PCR and nucleotide sequence analysis of part of the carboxyl-terminal region of the nucleoprotein gene of measles viruses obtained directly from clinical samples or from infected cell cultures during this epidemic classified all as wild-type of genotype D6. As the genotype D6 was identified in different Brazilian states, this study demonstrated that this genotype was circulating in Brazil during the 1997 epidemic.
Assuntos
Surtos de Doenças , Genoma Viral , Sarampo/virologia , Morbillivirus/genética , Adulto , Sequência de Bases , Brasil/epidemiologia , Sequência Consenso , Genótipo , Humanos , Lactente , Sarampo/epidemiologia , Sarampo/urina , Epidemiologia Molecular , Dados de Sequência Molecular , Morbillivirus/química , Morbillivirus/classificação , Nasofaringe/virologia , Nucleoproteínas/genética , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Sera from infants aged 5 to 11 months and from their mothers were used to investigate the level and duration of transplacentally derived measles antibody. The infants of foreign-born, inner-city mothers were more likely to have measles antibody and were less likely to get measles. Infants of foreign-born mothers, because they are less likely to respond to measles vaccine, may require different vaccine strategies than infants of mothers born in the United States.
Assuntos
Anticorpos Antivirais/sangue , Vírus do Sarampo/imunologia , Sarampo/etnologia , Feminino , Georgia , Humanos , Lactente , Sarampo/imunologia , Mães , Saúde da População UrbanaRESUMO
An infant boy with a congenital immunodeficiency had fatal disseminated measles after administration of a live attenuated measles vaccine. This rare complication was confirmed with molecular virologic techniques. Although efforts to expand availability of vaccinations are critically important, caution is warranted in children with potentially severe immunologic dysfunction.