RESUMO
Diazinon (DZN) is an insecticide extensively used to control pests in crops and animals. However, its indicriminated use may lead to liver damage in animals and humans. This study aimed to evaluate the toxicity of DZN (25-150 µM) on human hepatoblastoma (HepG2) cells after 24 and 48 h of exposure and the role of its biotransformation on the toxicological potential. We also tested the protective effect of tetrahydrocurcumin (THC), an antioxidant agent, in the DZN-induced citotoxicity. DZN caused cytotoxicity in the HepG2 cells, inhibiting cell proliferation and reducing cell viability in a dose- and time-dependent manner. The pre-incubation of HepG2 cells with chemical inducers of cytochrome P450 monooxygenase 3-methylcholanthrene and phenobarbital resulted in a further decrease of cell viability associated with DZN exposure. In addition, the metabolite diazoxon was more toxic than DZN. Our results also revealed that THC alleviated DZN-induced cytotoxicity and reactive oxygen and nitrogen species (RONS) generation in HepG2 cells. In conclusion, our data provide novel insights into the involvement of biotransformation in the mechanisms of DZN-induced cytotoxicity and suggest that amelioration of RONS accumulation might be involved in the protective effect of THC on DZN-induced liver injury.
RESUMO
Subspecialties are a worldwide reality. Plastic surgery, as a specialty, is increasingly subdividing. Thispaper refers to outpatient sector, making comments on it and emphasizing some requirements and advantages of this type of procedure.
Assuntos
Procedimentos Cirúrgicos Ambulatórios , Cirurgia Plástica/métodos , Procedimentos de Cirurgia Plástica , MétodosRESUMO
Trata-se de uma revisao da literatura e um relato de caso de um mixoma de maxila em crianca de um ano e tres meses submetida a maxilarectomia parcial e reconstrucao do assoalho da orbita onde foi utilizado fio de Kirchner para a formacao do rebordo orbitario e sustentacao de tela de polipropileno ancorada com fios de aco 00 no osso zigomatico e nasal, observa-se o resultado apos um ano e oito meses de pos-operatorio