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Gurltia paralysans is a nematode first described in 1933 in Chile, causing a syndrome called feline crural parasitic paraplegia. Insects, mollusks, frogs, lizards, and rodents are paratenic hosts of this nematode, and cats probably become infected by ingesting them. This report aims to discuss the main anatomopathological findings of gurltiosis in a cat submitted to necropsy in a laboratory in Ribeirão Preto city, São Paulo state, southeastern Brazil, being the first case reported in this state. The main necroscopic findings were extramedullary reddened areas below the leptomeninges in the cervical and lumbar segments of the spinal cord. The histopathological examination showed marked thickening of the leptomeninges in the lumbar segment of the spinal cord, with marked neovascularization and fibrosis associated with eosinophilic and mononuclear inflammatory infiltration, as well as the presence of intravascular nematodes. The diagnosis of this lesion was chronic segmental meningomyelitis associated with intralesional parasites. Although uncommon, feline gurltiosis is a neglected parasitic disease that should be included as a differential diagnosis of paraparesis in domestic cats.(AU)

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O aneurisma é uma dilatação anormal e permanente das artérias, resultante do enfraquecimento da parede do vaso adelgaçamento da camada média e enfraquecimento da camada elástica. Em animais, a maioria dos casos de aneurisma tem origem idiopática e são detectados acidentalmente durante a necropsia. O objetivo deste trabalho é relatar um caso de aneurisma aórtico com trombose associada em Bugio-preto(Alouatta caraya), bem como seus aspectos patológicos. O animal era adulto, macho, pertencente ao Centro Nacional de Primatas (CENP), na cidade de Ananindeua-PA, foi encaminhado para exame necroscópico para investigação da causa mortis. No histórico do animal, não constava qualquer enfermidade. O animal apresentava bom escore de condição corporal com preservação da topografia anatômica dos órgãos. Entretanto, observou-se presença de aumento de volume localizado em aorta torácica, a 1,4 cm da base do coração. Na abertura aórtica foi observado dilatações de tamanhos variados e, no interior da maior dilatação, notou-se uma estrutura de coloração vermelho escuro, aderida, de aspecto seco e superfície áspera, medindo 1,5 cm. Aneurismas aórticos em primatas não humanos não são comuns, porém já foram reportados na literatura. O diagnóstico precoce utilizando exames complementares é importante, porém, ainda há recursos não empregados na rotina veterinária tornando ainda mais difícil o diagnóstico e prevenção. Por isso, na medicina veterinária, os aneurismas são detectados acidentalmente durante a necropsia. Com base nos achados anatomopatológicos, concluiu-se que o animal veio a óbito por trombose associada a aneurisma aórtico.

An aneurysm is an abnormal and permanent dilation of the arteries, resulting from the weakening of the vessel wall.thinning of the middle layer and weakening of the elastic layer. In animals, most cases of aneurysm are idiopathic. This paper aimed to report a case of aortic aneurysm with associated thrombosis in a black-and-gold howler monkey(Alouatta caraya), as well as its pathological aspects. The animal was an adult, male, belonging to the National Primate Center (CENP), in the city of Ananindeua-PA, that was referred for necroscopic examination to investigate the causa mortis. In the animal's history, there was no disease. The animal had a good body condition score with preservation of the anatomical topography of the organs. However, there was an increase in volume located in the thoracic aorta, 1.4 cm from the base of the heart. In the aortic opening, dilations of different sizes were observed, and inside the largest dilatation, a structure of dark red color, adhered, with a dry appearance and rough surface, measuring 1.5 cm was noted in addition to dilations of different sizes. Inside the largest cavitation, a dark red structure was observed, adhered, with a dry appearance and rough surface, measuring 1.5 cm. Aortic aneurysms in non-human primates are incommon, but have been reported in the literature. Early diagnosis using complementary exams is important, however, there are still resources not used in the veterinary routine, making diagnosis and prevention even more difficult. Therefore, in veterinary medicine, aneurysms are accidentally detected during necropsy. Based on the anatomopathological findings, it was concluded that the animal died due to thrombosis associated with an aortic aneurysm.

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BACKGROUND: The thymus is necessary for the differentiation of T cells, a process that is regulated by the type of antigens found in thymocytes, the environment of surrounding cells and the thymus architecture. There is evidence that infectious diseases may result in morphological changes in this organ, such as premature atrophy and decreased thymocyte proliferation, that can affect the immune response. OBJECTIVES: We characterised the morphology and tissue distribution of haematopoietic and stromal cells in the thymuses of dogs naturally infected with Leishmania infantum, with the aim to determine the changes that may contribute to the pathophysiology of the disease. METHODS: Thymus samples were collected from 15 animals (aged 6 months to 5 years) ELISA-positive for leishmaniasis and from 10 dogs from non-endemic regions for leishmaniasis whose death was not related to infectious causes. The samples were submitted to histological processing and staining with Haematoxylin-Eosin to assess thymic morphometry and histopathological changes. Masson's trichrome staining was used to quantify the connective tissue present (collagen). The immunohistochemical method was used to determine the cellular constitution of the thymus, using antibodies that aimed at marking T lymphocytes (anti-CD3), B lymphocytes (anti-CD79a), macrophages (anti- MAC387), mesenchymal cells (anti-vimentin), epithelial cells (anti-cytokeratin), cells in mitosis (anti-Ki67) and cells in apoptosis (anti-caspase-3). RESULTS: The histopathological evaluation of infected dogs showed more signs consistent with thymus atrophy, including decreased parenchyma, infiltration of adipose and connective tissue near the capsule and between the lobules, lymphoid rarefaction mainly in the cortical region and loss of the cortical-medullary demarcation. In addition, we observed a decrease in the amounts of CD3 + T lymphocytes, macrophages (MAC387) and Ki67-positive cells and an increase in the number of cells positive for cytokeratin and CD79a (B lymphocytes). Finally, the parasite was detected in 46% of infected thymuses and may contribute for the observed changes. CONCLUSIONS: Apparently, leishmaniasis, like other infectious diseases, causes atrophy of the thymus and depletion of thymocytes with a relative increase in thymus epithelial cells. These morphological changes in the normal organisation of the thymus by mechanisms not yet well known may result in the abnormal release of T cells, with consequent damage to the host's immune response.

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The aim of this study was to evaluate the histopathological changes to the mammary gland that occur in female dogs with visceral leishmaniosis and to correlate the findings with the parasite load, inflammatory cell profile in mammary tissue and serum progesterone levels. For this, 20 adult female dogs that were naturally infected with Leishmania infantum, not spayed, not pregnant and free from mammary tumors were used. They were divided into two groups: G1 (n = 9) with high serum progesterone levels and G2 (n = 11) with low serum progesterone levels. The parasite load and the immunophenotype of leukocytes infiltrated into the mammary tissue (CD3, CD4, CD8 and MCA874) were evaluated using the immunohistochemical technique. In the mammary gland, chronic inflammatory infiltrate was mainly found in G1, sometimes associated with granulomatous inflammation, higher parasite load and higher density of cells immunolabeled for CD3, CD4, CD8 and MCA874. A significant positive correlation (p < 0.05) was observed between the parasite load and the immunolabeled leukocytes. The influence of the serum progesterone level in the mammary gland of infected female dogs can contribute to the maintenance of an immunosuppressive cell profile and favor the persistence of the parasite in this site.

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Poly(lactic acid) (PLA) and poly(ε-caprolactone) (PCL) are two important aliphatic esters known for their biodegradability and bioresorbability properties; the former is stiffer and brittle while the smaller modulus of the latter allows a suitable elongation. The new biomaterials being developed from the blend of these two polymers (PLA and PCL) is opportune due to the reducing interfacial tension between their immiscible phases. In a previous study, PLA/PCL immiscible blend when compatibilized with poly(ε-caprolactone-b-tetrahydrofuran) resulted in enhanced ductility and toughness no cytotoxic effect invitrotests. There is little published data on the effect of poly(ε-caprolactone-b-tetrahydrofuran) on PLA and PCL biocompatibility and biodegradabilityin vivotests. This study focuses on evaluating the behavioral response and polymer-tissue interaction of compatibilized PLA/PCL blend compared to neat PLA implanted via intraperitoneal (IP) and subcutaneous (SC) in male Wistar rats, distributed in four experimental groups: neat PLA, PLA/PCL blend, sham, and control at 2-, 8- and 24-weeks post-implantation (WPI). An open-field test was performed to appraise emotionality and spontaneous locomotor activity. Histopathological investigation using hematoxylin-eosin (H&E) and picrosirius-hematoxylin (PSH) was used to assess polymer-tissue interaction. Modifications in PLA and the PLA/PCL blend's surface morphology were determined by scanning electron microscopy (SEM). PLA group defecated more often than PLA/PCL rats 2 and 8 WPI. Conjunctive capsule development around implants, cell adhesion, angiogenesis, and giant cells of a foreign body to the biomaterial was observed in light microscopy. Both groups displayed a fibrous reaction along with collagen deposition around the biomaterials. In the SEM, the images showed a higher degradation rate for the PLA/PCL blend in both implantation routes. The polymers implanted via IP exhibited a higher degradation rate compared to SC. These findings emphasize the biocompatibility of the PLA/PCL blend compatibilized with poly(ε-caprolactone-b-tetrahydrofuran), making this biopolymer an acceptable alternative in a variety of biomedical applications.

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The goal of this study was to report the occurrence and histopathological aspects of uterine adenomyosis in bitches and queens. The sample consisted of 200 uteri from bitches (n =100) and queens (n = 100) obtained from elective ovarian-salpingohysterectomies. The animals were classified according to age, having given birth, and contraceptive use. Samples of the uterine horns were collected following routine histological processing using slides stained with hematoxylin and eosin. The data obtained were analysed using the chi-square test, with 5% significance level. Uterine adenomyosis was confirmed in 12% of bitches and 3% of queens. Age had no influence on the occurrence of this disease; however, not having given birth and the use of contraceptives favoured its appearance in bitches. Microscopically, portions of endometrial glands and stroma were present in the circular and vascular myometrial strata, with normal and cystic characteristics and variations in the glandular epithelium. There was cystic-type luminal content, such as calcification, hyaline substance, polyps, and mucin. This study made it possible to conclude that uterine adenomyosis occurs more frequently in bitches than in queens, regardless of age groups. Nulliparous bitches using contraceptives were the most affected. This pathology showed variations with respect to location and histopathological aspects, with the cystic type exhibiting calcification, hyaline substance, polyps, and mucin inside the lumen. These findings are being reported for the first time in the veterinary literature.

Objetivou-se com essa pesquisa relatar a ocorrência e os aspectos histopatológicos da adenomiose uterina em cadelas e gatas. Foram utilizados 200 úteros, de cadelas (n=100) e gatas (n=100), procedentes de ovariosalpingohisterectomias eletivas. Os animais foram classificados segundo a faixa etária, parição e uso de contraceptivos. Amostras dos cornos uterinos foram coletadas, seguindo-se ao processamento histológico de rotina, sendo as lâminas coradas pela hematoxilina e eosina. Os dados obtidos foram analisados pelo teste Qui-quadrado, com significância de 5%. A adenomiose uterina foi identificada em 12% das cadelas e 3% das gatas. Não houve influência na ocorrência dessa enfermidade quanto a faixa etária, porém a ausência de parição e o uso de contraceptivos favoreceram o aparecimento em cadelas. Microscopicamente, porções de glândulas e estroma endometrial estavam presentes no estrato miometrial circular e vascular, ocorrendo o tipo normal, cístico e variações no epitélio glandular. Houve presença de conteúdo luminal no tipo cístico, como calcificação, substância hialina, pólipos e mucina. Com esse estudo foi possível concluir que a adenomiose uterina ocorre com maior frequência em cadelas do que em gatas, independente da faixa etária. Cadelas nulíparas que utilizaram contraceptivos foram as mais afetadas. Essa patologia mostrou variações quanto a localização e aspecto histopatológico, exibindo no tipo cístico, calcificação, substância hialina, pólipos e mucina no interior do lúmen, sendo estes achados relatados pela primeira vez na literatura veterinária.

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The goal of this study was to report the occurrence and histopathological aspects of uterine adenomyosis in bitches and queens. The sample consisted of 200 uteri from bitches (n =100) and queens (n = 100) obtained from elective ovarian-salpingohysterectomies. The animals were classified according to age, having given birth, and contraceptive use. Samples of the uterine horns were collected following routine histological processing using slides stained with hematoxylin and eosin. The data obtained were analysed using the chi-square test, with 5% significance level. Uterine adenomyosis was confirmed in 12% of bitches and 3% of queens. Age had no influence on the occurrence of this disease; however, not having given birth and the use of contraceptives favoured its appearance in bitches. Microscopically, portions of endometrial glands and stroma were present in the circular and vascular myometrial strata, with normal and cystic characteristics and variations in the glandular epithelium. There was cystic-type luminal content, such as calcification, hyaline substance, polyps, and mucin. This study made it possible to conclude that uterine adenomyosis occurs more frequently in bitches than in queens, regardless of age groups. Nulliparous bitches using contraceptives were the most affected. This pathology showed variations with respect to location and histopathological aspects, with the cystic type exhibiting calcification, hyaline substance, polyps, and mucin inside the lumen. These findings are being reported for the first time in the veterinary literature.(AU)

Objetivou-se com essa pesquisa relatar a ocorrência e os aspectos histopatológicos da adenomiose uterina em cadelas e gatas. Foram utilizados 200 úteros, de cadelas (n=100) e gatas (n=100), procedentes de ovariosalpingohisterectomias eletivas. Os animais foram classificados segundo a faixa etária, parição e uso de contraceptivos. Amostras dos cornos uterinos foram coletadas, seguindo-se ao processamento histológico de rotina, sendo as lâminas coradas pela hematoxilina e eosina. Os dados obtidos foram analisados pelo teste Qui-quadrado, com significância de 5%. A adenomiose uterina foi identificada em 12% das cadelas e 3% das gatas. Não houve influência na ocorrência dessa enfermidade quanto a faixa etária, porém a ausência de parição e o uso de contraceptivos favoreceram o aparecimento em cadelas. Microscopicamente, porções de glândulas e estroma endometrial estavam presentes no estrato miometrial circular e vascular, ocorrendo o tipo normal, cístico e variações no epitélio glandular. Houve presença de conteúdo luminal no tipo cístico, como calcificação, substância hialina, pólipos e mucina. Com esse estudo foi possível concluir que a adenomiose uterina ocorre com maior frequência em cadelas do que em gatas, independente da faixa etária. Cadelas nulíparas que utilizaram contraceptivos foram as mais afetadas. Essa patologia mostrou variações quanto a localização e aspecto histopatológico, exibindo no tipo cístico, calcificação, substância hialina, pólipos e mucina no interior do lúmen, sendo estes achados relatados pela primeira vez na literatura veterinária.(AU)

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Background: Intestinal neoplasms are uncommon in dogs and adenocarcinoma is the main histological type found. Thisneoplasm presents slow growth and high capacity of causing metastasis. Histologically speaking, neoplasm cells can presentsolid, tubular, papillary arrangement and note amorphous extra-cellular material. Clinically observed tenesmus, diarrhea,dyskinesia, hematochezia, mane, protrusion of the anus, weight loss, anorexia. The occurrence and clinicopathologicalaspects of tumors in dogs gastrointestinal tract, the rectal segment, remains poorly understood. Accordingly, the aim ofthe present study is to report a case on infiltrative rectal adenocarcinoma diagnosed in a dog.Case: A 7-year-old male dog representative of the Fila Brasileiro breed was presented to the Veterinary Hospital of University Federal Rural of Amazonia, with history of hyperthermia, anorexia, apathy and tenesmus. Imaging examinationsdepicted prostatomegaly. Exploratory laparotomy was performed and showed the thickening and hardening of the rectumsegment. The animal was subjected to euthanasia. Necroscopy showed increased rectal perimeter; the mucosa in its opening presented atypical cerebroid aspect and irregular surface, and areas dark red. The rectal segment depicted a thick wallof white color, irregular limits covering the muscular and adjacent sub-mucosa. The peri-rectal adipose tissue presentedpoor delimitation with the rectum, multiple greyish and reddish areas. Increased prostate and iliac lymph, and multi nodeof regular limits in the lungs. The histology of the rectal tissue depicted epithelium with differentiated neoformation,composed of atypical cells; nuclear anisocytosis, anisocariasis and hyperchromasia placed in small islands, cords or tubular formation. Neoplasm growth was unorganized and of infiltrative character. Some areas presented mucosal patterncells with Signal Ring morphologic. Multiple rectal blood...(AU)

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Background: Multilobular tumor of bone (MTB) is a primary bone neoplasm, uncommon in dogs. This tumor was called ofmany names for a long time, as: chondroma rodens, multilobular osteochondrosarcoma, multilobular osteoma, multilobularchondroma, calcificating aponeurotic fibroma, although MTB was preferred chosen, because these other name could becorrelated with humans tumors. This tumor is observed specially in skull bone, although it was reported in zygomaticarc, hard palate, axilla, spine and penis. Mostly happen in big breeds dogs, and middle to old age patients. Clinical signsdepending of region and how aggressive the tumor is, usually are related to compression of any structure. The growth ofMTB is frequently slow and progressive, locally invasive, occurring relapse after surgical revomal, although the tumorhas low to moderate metastatic potential. The aim of this study is to report a case of MTB in a female dog and describeanatomopathological changes.Case: A female dog, mixed breed, 13 year-old, of middleweight was admitted in Pathology Department of College of Agricultural Sciences and Veterinary Medicine (FCAV-Unesp), Campus of Jaboticabal - SP, to be undergone to necropsy. Thepatient have never shown any epileptic crisis or neurologic signs. In macroscopic examination was found a mass in skull,which invaded the orbit and frontal sinus, but it was not invading brain cavity. The neoplasm had and irregular surface, firmconsistent, color was white mixed to red areas, after cut it was granular and rough, and had some point mineralized areas.The lobs of lung had much firm masses, colored gray to white. In cytology it was observed fusiform to polyhedric isolatedmesenchymal cells, moderated pleomorphic, basophilic cytoplasm, thin granulated nuclear chromatin, and visible nucleolithat was involved by eosinophilic extracellular matrix...(AU)

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Background: Multilobular tumor of bone (MTB) is a primary bone neoplasm, uncommon in dogs. This tumor was called ofmany names for a long time, as: chondroma rodens, multilobular osteochondrosarcoma, multilobular osteoma, multilobularchondroma, calcificating aponeurotic fibroma, although MTB was preferred chosen, because these other name could becorrelated with humans’ tumors. This tumor is observed specially in skull bone, although it was reported in zygomaticarc, hard palate, axilla, spine and penis. Mostly happen in big breeds dogs, and middle to old age patients. Clinical signsdepending of region and how aggressive the tumor is, usually are related to compression of any structure. The growth ofMTB is frequently slow and progressive, locally invasive, occurring relapse after surgical revomal, although the tumorhas low to moderate metastatic potential. The aim of this study is to report a case of MTB in a female dog and describeanatomopathological changes.Case: A female dog, mixed breed, 13 year-old, of middleweight was admitted in Pathology Department of College of Agricultural Sciences and Veterinary Medicine (FCAV-Unesp), Campus of Jaboticabal - SP, to be undergone to necropsy. Thepatient have never shown any epileptic crisis or neurologic signs. In macroscopic examination was found a mass in skull,which invaded the orbit and frontal sinus, but it was not invading brain cavity. The neoplasm had and irregular surface, firmconsistent, color was white mixed to red areas, after cut it was granular and rough, and had some point mineralized areas.The lobs of lung had much firm masses, colored gray to white. In cytology it was observed fusiform to polyhedric isolatedmesenchymal cells, moderated pleomorphic, basophilic cytoplasm, thin granulated nuclear chromatin, and visible nucleolithat was involved by eosinophilic extracellular matrix...

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