RESUMO
Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for treating infectious bovine rhinotracheitis disease.
Assuntos
Vetores Genéticos/imunologia , Herpesvirus Bovino 1/imunologia , Herpesvirus Humano 1/imunologia , Rinotraqueíte Infecciosa Bovina/imunologia , Proteínas Virais/imunologia , Animais , Anticorpos Antivirais/sangue , Western Blotting/veterinária , Bovinos , Feminino , Herpesvirus Bovino 1/genética , Herpesvirus Humano 1/genética , Imunidade Humoral/imunologia , Imunização/métodos , Imunização/veterinária , Rinotraqueíte Infecciosa Bovina/prevenção & controle , Rinotraqueíte Infecciosa Bovina/virologia , Camundongos , Camundongos Endogâmicos BALB C , Testes de Neutralização/veterinária , Organismos Livres de Patógenos Específicos , Proteínas Virais/genética , Vacinas Virais/imunologiaRESUMO
The aim of this work was to detect serologic evidence of influenza virus infections in South American fur seals (Arctocephalus australis) that inhabit the Uruguayan coast. In 29 of 37 serum samples that were analyzed, we identified antibodies to at least one of the following antigens: H1N1 (A/NewCaledonia/20/99), B/Beijing/184/93-like viruses, B/Hong Kong/330/01, and B/Sichuan/379/99 by means of the hemagglutination inhibition assay (HAI). Results confirmed that influenza A viruses circulate in marine mammals and also showed, for the first time, indirect evidence of influenza B infections in Arctocephalus australis.
Assuntos
Anticorpos Antivirais/sangue , Otárias/virologia , Vírus da Influenza A/imunologia , Vírus da Influenza B/imunologia , Animais , Feminino , Testes de Inibição da Hemaglutinação/veterinária , Masculino , Estudos Soroepidemiológicos , Uruguai/epidemiologiaRESUMO
First identified in 2001, the human metapneumovirus (hMPV), is a respiratory tract pathogen that affects young children, elderly, and immunocompromised patients. The present work represents the first serologic study carried out in Uruguay. It was performed with the purpose of obtaining serological evidence of hMPV circulation in Uruguay and to contribute to the few serologic reports described until now. Sixty nine serum samples collected between 1998 and 2001 by vein puncture from patients without respiratory symptoms or underlying pathology aged 6 days to 60 years were examined using an indirect immunofluorescence assay (IFA). The global seropositivity rate of the samples was 80 percent (55/69). Rates of 60 percent (15/25) and 91 percent (40/44) were observed for the pediatric and adult cohorts, respectively. Results obtained from a longitudinal analysis of 6 children aged 6 days to 18 months are discussed. These results are a clear evidence of hMPV circulation in Uruguay, at least since 1998, and reinforce the previous data on worldwide circulation of this virus.
Assuntos
Recém-Nascido , Lactente , Adolescente , Adulto , Pessoa de Meia-Idade , Humanos , Anticorpos Antivirais/sangue , Metapneumovirus/isolamento & purificação , Infecções por Paramyxoviridae/epidemiologia , Infecções por Paramyxoviridae/virologia , Técnica Indireta de Fluorescência para Anticorpo , Estudos Longitudinais , Metapneumovirus/genética , Metapneumovirus/imunologia , Prevalência , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Estudos Soroepidemiológicos , Uruguai/epidemiologiaRESUMO
First identified in 2001, the human metapneumovirus (hMPV), is a respiratory tract pathogen that affects young children, elderly, and immunocompromised patients. The present work represents the first serologic study carried out in Uruguay. It was performed with the purpose of obtaining serological evidence of hMPV circulation in Uruguay and to contribute to the few serologic reports described until now. Sixty nine serum samples collected between 1998 and 2001 by vein puncture from patients without respiratory symptoms or underlying pathology aged 6 days to 60 years were examined using an indirect immunofluorescence assay (IFA). The global seropositivity rate of the samples was 80% (55/69). Rates of 60% (15/25) and 91% (40/44) were observed for the pediatric and adult cohorts, respectively. Results obtained from a longitudinal analysis of 6 children aged 6 days to 18 months are discussed. These results are a clear evidence of hMPV circulation in Uruguay, at least since 1998, and reinforce the previous data on worldwide circulation of this virus.
Assuntos
Anticorpos Antivirais/sangue , Metapneumovirus , Infecções por Paramyxoviridae/epidemiologia , Adolescente , Adulto , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Lactente , Recém-Nascido , Estudos Longitudinais , Metapneumovirus/genética , Metapneumovirus/imunologia , Pessoa de Meia-Idade , Infecções por Paramyxoviridae/virologia , Prevalência , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Estudos Soroepidemiológicos , Uruguai/epidemiologiaRESUMO
Outbreaks of human respiratory syncytial virus (HRSV) are the leading cause of serious acute lower respiratory viral disease in many countries in different continents. Data on clinical and epidemiological aspects of HRSV infections in this country have been reported, but there is lack of data regarding the molecular epidemiology of this virus in Salvador. The genetic variability of HRSV isolated during an outbreak in Salvador, Brazil (1999) has been analysed. Partial sequences of the G protein gene of 13 isolates from antigenic group A and 4 isolates from antigenic group B of HRSV were determined. Nucleotide sequences of C-terminal G gene were compared to sequences of HRSV isolates from countries of South America and from the rest of the world available at the GenBank. Brazilian group A and B isolates were clustered into previously characterised genotypes: GA5, GA2, GA7, and GB3, SAB3, respectively. This is the first study of GA7 and SAB3 genotypes circulation in South American countries. It is interesting to point out that viruses isolated in Salvador appear to be closer related with those from Montevideo-Uruguay and Buenos Aires, Argentina strains, suggesting circulation of similar strains among different South American countries in different seasons. Moreover, viruses closely related genetically circulated in the same year in Salvador and distant places such as Mozambique, supporting the previous suggestion on the complexity of HRSV strain circulation patterns, and the high capability of HRSV spreading world-wide.