RESUMO
Primary sarcomas of the major blood vessels can be classified based on location in relationship to the wall or by histologic type. Angiosarcomas are malignant neoplasms that arise from the endothelial lining of the blood vessels; those arising in the intimal compartment of pulmonary artery are rare. We report a case of pulmonary artery angiosarcoma in a 36-year old female with pulmonary masses. The patient had no other primary malignant neoplasm, thus excluding a metastatic lesion. Gross examination revealed a thickened right pulmonary artery and a necrotic and hemorrhagic tumor, filling and occluding the vascular lumen. The mass extended distally, within the pulmonary vasculature of the right lung. Microscopically, an intravascular undifferentiated tumor was identified. The tumor cells showed expression for vascular markers VEGFR, VEGFR3, PDGFRa, FGF, Ulex europaeus, FVIII, FLI-1, CD31 and CD34; p53 was overexpressed and Ki67 proliferative rate was increased. Intravascular angiosarcomas are aggressive neoplasms, often associated with poor outcome.
Assuntos
Erros de Diagnóstico , Hemangiossarcoma/patologia , Histiocitoma Fibroso Maligno/patologia , Artéria Pulmonar/patologia , Neoplasias Vasculares/patologia , Adulto , Biomarcadores Tumorais/análise , Biópsia , Proliferação de Células , Quimioterapia Adjuvante , Feminino , Hemangiossarcoma/química , Hemangiossarcoma/terapia , Humanos , Imuno-Histoquímica , Fenótipo , Pneumonectomia , Valor Preditivo dos Testes , Artéria Pulmonar/química , Artéria Pulmonar/cirurgia , Tomografia Computadorizada por Raios X , Resultado do Tratamento , Neoplasias Vasculares/química , Neoplasias Vasculares/terapiaRESUMO
Basal cell proliferations within the prostate gland encompass a group of benign and malignant entities. Although basal cell hyperplasia is a common finding, basal cell carcinoma of the prostate gland is a rare tumor that can be mistaken by a benign condition and represents a diagnostic problem in genitourinary pathology. We report a case of basal cell carcinoma in a previously healthy 65-year-old man with urinary symptoms and low prostate-specific antigen. The microscopic findings are presented and the use of immunohistochemical markers classifying basal cell lesions of the prostate discussed.
Assuntos
Biomarcadores Tumorais/análise , Carcinoma Basocelular/diagnóstico , Neoplasias da Próstata/diagnóstico , Idoso , Biomarcadores Tumorais/metabolismo , Carcinoma Basocelular/química , Carcinoma Basocelular/imunologia , Carcinoma Basocelular/patologia , Diagnóstico Diferencial , Humanos , Masculino , Próstata/metabolismo , Próstata/patologia , Antígeno Prostático Específico/análise , Antígeno Prostático Específico/metabolismo , Neoplasias da Próstata/química , Neoplasias da Próstata/imunologia , Neoplasias da Próstata/patologia , Sensibilidade e EspecificidadeRESUMO
AIM: To investigate the presence of human papillomavirus (HPV) in esophageal squamous papilloma (ESP) and determine p16, p53 and Ki67 expression in a Mexican cohort. METHODS: Nineteen cases diagnosed as ESP, corresponding to 18 patients were reviewed; nineteen cases of normal esophageal mucosa were used as negative controls. HPV detection was performed by amplified chromogenic in situ hybridization (ACISH) using a wide spectrum-cocktail probe and PCR. RESULTS: The average age at presentation was 46.3 years (range 28-72 years). Patients included four (22.22%) males and 14 (77.77%) females. The most frequent location was upper third (11 cases), followed by middle third (3 cases) and unknown site (5 cases). Immunohistochemistry (IHC) revealed basal and focal p53 expression in 17 cases (89%); p16 was expressed in eight cases (42.10%) and the Ki67 index ranged from 10% to 30%. HPV was detected in 14 out of 16 cases (87.5%) by ACISH: Twelve showed diffuse nuclear patterns and two showed granular patterns. HPV DNA was identified by PCR in 12 out of 14 cases (85.7%). Low-risk HPV types were detected in the most of the cases. CONCLUSION: This study provides identification of HPV infection in almost 80% of ESP using either ACISH or PCR; overall, all of these lesions show low expression of cell-cycle markers. We suggest ACISH as an alternative diagnostic tool for HPV detection in ESP.
Assuntos
Neoplasias Esofágicas/virologia , Papiloma/virologia , Papillomaviridae/classificação , Papillomaviridae/patogenicidade , Adulto , Idoso , Estudos de Casos e Controles , Estudos de Coortes , Inibidor p16 de Quinase Dependente de Ciclina/metabolismo , Neoplasias Esofágicas/metabolismo , Neoplasias Esofágicas/patologia , Feminino , Papillomavirus Humano 11/patogenicidade , Papillomavirus Humano 16/patogenicidade , Papillomavirus Humano 18/patogenicidade , Papillomavirus Humano 6/patogenicidade , Humanos , Hibridização In Situ , Antígeno Ki-67/metabolismo , Masculino , México , Pessoa de Meia-Idade , Papiloma/metabolismo , Papiloma/patologia , Reação em Cadeia da Polimerase , Estudos Retrospectivos , Proteína Supressora de Tumor p53/metabolismoRESUMO
Langerhans cell sarcoma (LCS) is a neoplastic proliferation of Langerhans cells that occurs in lymph nodes, liver, skin, spleen, lung, and bone. We report a case of LCS in a 47-year-old man with a 6-month history of scalp mass and cervical lymphadenopathy. Clinical and pathologic data were available. A histologic examination demonstrated a proliferation of cells with malignant cytologic features. Because of its poorly differentiated morphologic features, hematologic and nonhematologic entities were ruled out by immunohistochemical screening with a broad panel of antibodies. Ultrastructural studies demonstrating Birbeck granules and consistent expression of CD1a, S-100 protein, and langerin by immunohistochemistry were helpful in identifying the Langerhans cell origin.