RESUMO
YABBY genes encode specific TFs of seed plants involved in development and formation of leaves, flowers, and fruit. In the present work, genome-wide and expression analyses of the YABBY gene family were performed in six species of the Fragaria genus: Fragaria × ananassa, F. daltoniana, F. nilgerrensis, F. pentaphylla, F. viridis, and F. vesca. The chromosomal location, synteny pattern, gene structure, and phylogenetic analyses were carried out. By combining RNA-seq data and RT-qPCR analysis we explored specific expression of YABBYs in F. × ananassa and F. vesca. We also analysed the promoter regions of FaYABBYs and performed MeJA application to F. × ananassa fruit to observe effects on gene expression. We identified and characterized 25 YABBY genes in F. × ananassa and six in each of the other five species, which belong to FIL/YAB3 (YABBY1), YAB2 (YABBY2), YAB5 (YABBY5), CRC, and INO clades previously described. Division of the YABBY1 clade into YABBY1.1 and YABBY1.2 subclades is reported. We observed differential expression according to tissue, where some FaYABBYs are expressed mainly in leaves and flowers and to a minor extent during fruit development of F. × ananassa. Specifically, the FaINO genes contain jasmonate-responsive cis-acting elements in their promoters which may be functional since FaINOs are upregulated in F. × ananassa fruit under MeJA treatment. This study suggests that YABBY TFs play an important role in the development- and environment-associated responses of the Fragaria genus.
Assuntos
Ciclopentanos , Diploide , Fragaria , Regulação da Expressão Gênica de Plantas , Oxilipinas , Filogenia , Proteínas de Plantas , Fatores de Transcrição , Fragaria/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Ciclopentanos/metabolismo , Ciclopentanos/farmacologia , Oxilipinas/farmacologia , Oxilipinas/metabolismo , Frutas/genética , Frutas/crescimento & desenvolvimento , Poliploidia , Acetatos/farmacologia , Regiões Promotoras Genéticas/genética , Sintenia , Família MultigênicaRESUMO
OBJETIVO: El traumatismo grave es la causa principal de muerte en pacientes menores de 40 años, siendo el trauma craneoencefálico la primera causa de discapacidad severa, mientras que la hemorragia se mantiene como principal causa prevenible de muerte. El objetivo del presente trabajo es describir la casuística de los pacientes atendidos en la Unidad de Cuidados Intensivos Adultos del Centro de Trauma Hospital Corazón de Jesús de la ciudad de El Alto en La Paz (Bolivia). METODOLOGÍA: Estudio retrospectivo. Se incluyen todos los pacientes internados en la Unidad de Cuidados Intensivos Adultos del Centro de Trauma Hospital Corazón de Jesús, Unidad Polivalente, ingresados en el periodo 01 Octubre 2016-01 Abril de 2019. RESULTADOS: Se ingresaron 485 pacientes, 273 (56%) varones así como 212 (44%) mujeres, la sobrevida llega al 91% y el 9% de mortalidad corregida. La media de edad corresponde a 48 años (desviación estándar 19 años), la media de estancia en la Unidad de Cuidados Intensivos es de 112 horas (desviación estándar 87 horas). La mayor parte de pacientes proviene del Servicio de Urgencias y Quirófano, con diagnósticos de trauma craneoencefálico grave y hemorragia secundaria a trauma. CONCLUSIÓN: Este estudio muestra la casuística de una Unidad de Cuidados Intensivos de un Centro de Trauma, resultando imperativo describir la casuística concerniente a nuestros Centros Asistenciales con fines de la realización de investigación ulterior de forma multicéntrica.
OBJECTIVE: Severe trauma is leading cause of death in patients younger than 40 years, traumatic brain injury is also the leading cause of severe disability, while hemorrhage remains as main cause of preventable death. The objective of this paper is to describe the casuistic of patients treated in the Intensive Care Unit of Trauma Center Hospital Corazón de Jesús of El Alto City in La Paz (Bolivia). METHODOLOGY: Retrospective study. Includes all patients admitted at Intensive Care Unit, Polyvalent Unit, during the period 01 October 2016-01 April 2019. RESULTS: 485 patients were admitted, 273 (56%) male and 212 (44%) female, survival reaches 91% and 9% of adjusted mortality. Mean age is 48 years (standard deviation 19 years), mean length-of-stay in Intensive Care Unit is 112 hours (standard deviation 87 hours). The majority of patients arrived from the Emergency Department and Operating Room, with main diagnoses of severe traumatic brain injury and hemorrhage secondary to trauma. CONCLUSION: This study shows the casuistic of an Intensive Care Unit of a Trauma Center, resulting imperative to describe the casuistic of other Hospitals for the purpose of conducting further multicentric research.
Assuntos
Humanos , Centros de Traumatologia , Causas de Morte , Cuidados Críticos , Lesões Encefálicas Traumáticas , Pacientes , Estudos Retrospectivos , Diagnóstico , EmergênciasRESUMO
Control samples of scintillation liquids - Phaeozem soil mixtures were prepared with different scintillation liquids as the support electrolyte, Install Gel® XF, (Ultima Gold AB™ and Ultima Gold XR™), to construct the polarization curves, and to select the cell potential with the highest mass transfer to remove 24Na (15 h) and 99mTc (6 h) as radiotracers from polluted Phaeozem soil. During the electrokinetic treatment (EKT), the removal of radionuclides contained in scintillation liquids absorbed in Phaeozem soil, liquid phase was characterized by Gas Chromatography coupled with a Flame Ionization Detector (GC-FID) and Fourier Transform Infrared Spectrometry (FTIR), solids by FTIR, before and after the application of cell potential. In this sense, the support electrolyte was selected based on the highest current generated (1 mA), as in the case of scintillation liquid 50% Ultima Gold XR™ + 50% Water (1:1), which was used for 6 h in the presence of a mesh and a titanium rod, as anode and cathode, respectively. Finally, the removal percentage accumulated in the liquid phase after the EKT of Phaeozem soil polluted by 99mTc was 61% close to the anode after 4 h. It was also 61% for 24Na close to cathode after 2 h, and after 4 h it was 71.8%.
Assuntos
Modelos Químicos , Radioisótopos/análise , Poluentes Radioativos do Solo/análise , Adsorção , CinéticaRESUMO
Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface hydrophobicity of cell-associated proteins extracted with 3 mol/L urea, revealing proteins with a significant hydrophobic profile.
Assuntos
Helicobacter pylori/metabolismo , Hemina/metabolismo , Interações Hidrofóbicas e Hidrofílicas , Proteínas de Membrana/metabolismo , Aderência Bacteriana , Vermelho Congo/metabolismo , Eletroforese em Gel de Poliacrilamida , Etilenoglicol/farmacologia , Helicobacter pylori/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Cloreto de Lítio/farmacologia , Ligação Proteica , Cloreto de Sódio/farmacologia , Temperatura , Ureia/farmacologiaRESUMO
Enantiomeric alanine was covalently grafted onto modified gold electrodes with mercaptopropionic acid and PAMAM dendrimers G4.0 with amine terminal groups. Cyclic voltammetric experiments in the presence of monocarboxylic ferrocene as a probe molecule proved that the alanine (Ala) was immobilized as a monolayer on the gold electrodes. Electron transfer to Ru(NH3)6Cl3 in solutions of different pH was studied by cyclic voltammetry (CV). Changes in solution pH resulted in the variation of the charge state of the terminal group and surface pKa values were estimated on the basis of these results. Because of electrostatic interactions between the positive charged groups on the electrode surface and the Ala, enantioselective recognition was possible. The interaction between enantiomers can be proven with molecular simulation. The electro-oxidation peak current was linearly dependent on Ala concentration over the range 0-10 microM with slopes between 143 and 187 microAcm(-2)/microM. The detection limit (3sigma) was 0.4059 microM for PAMAM G4.0-D(+)Ala-L(-)Ala and 0.4172 microM for PAMAM G4.0-L(-)Ala-D(+)Ala.
Assuntos
Alanina/análise , Técnicas Eletroquímicas , Poliaminas/química , Alanina/química , Dendrímeros , Eletrodos , Ouro , Concentração de Íons de Hidrogênio , Oxirredução , Rutênio , Soluções , EstereoisomerismoRESUMO
In Mexico about 4,000 plant species have some medicinal use. The aim of this work was to evaluate the antimicrobial activity of six Mexican medicinal plants against fungi and Gram-positive and Gram-negative bacteria. Methanolic extracts were prepared from the Mexican medicinal plants Amphypteringium adstrigens, Castella tortuosa, Coutarea latiflora, Ibervillea sonorae, Jatropha cuneata, and Selaginella lepidophylla. The antibacterial and antifungal activities of the plants were determined by the broth microdilution method and the radial growth inhibition assay, respectively. All Mexican plants tested showed antimicrobial activity. Among the six plant extracts analyzed, J. cuneata showed the highest growth-inhibitory activity against fungi, Gram-positive and Gram-negative bacteria (J. cuneata > A. adstrigens > C. latiflora > C. tortuosa > I. sonorae approximately S. lepidophylla). Shigella flexneri and Staphylococcus aureus were the most susceptible bacteria to plant extracts. Complete inhibition of S. flexneri growth was observed with J. cuneata methanolic extract at 90 microg/mL. This plant extract also showed the strongest antifungal activity against Fusarium verticillioides and Aspergillus niger. Our data suggest that the medicinal plants tested have important antimicrobial properties. This is the first report describing the antimicrobial activities of several of the Mexican medicinal plants used in this study.
Assuntos
Antibacterianos/farmacologia , Antifúngicos/farmacologia , Extratos Vegetais/farmacologia , Plantas Medicinais/química , Antibacterianos/análise , Antifúngicos/análise , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Fungos/efeitos dos fármacos , Fungos/crescimento & desenvolvimento , México , Testes de Sensibilidade Microbiana , Extratos Vegetais/análiseRESUMO
The multiaperture scintillation sensor (MASS) has become a device widely employed to measure the altitude distribution of atmospheric turbulence. An empirical study is reported that investigates the dependence of the MASS results on the knowledge of the instrumental parameters. Also, the results of a side-by-side comparison of two MASS instruments are presented, indicating that MASS instruments permit measurements of the integrated seeing to a precision better than 0.05 arc sec and of the individual turbulence layer strength C(n)(2)(h)dh to better than 10(-14) m(1/3).
RESUMO
To assess the immunogenic and immunoprotective role of the extracellular lectin from Aeromonas veronii (MCBP), which has affinity for mucosal constituents such as mucin, lactoferrin, immunoglobulins and collagen, spotted sand bass (Paralabrax maculatofasciatus) were orally immunised either with soluble MCBP, adjuvant-conjugated MCBP or immobilised MCBP on latex microspheres. The results suggest that the MCBP is capable of eliciting protective immunity against A. veronii infections when administered orally. The highest mucosal immune response was elicited in fish immunised with MCBP covalently linked to cholera toxin B subunit (CTB) or to Escherichia coli heat-labile toxin (hLT). MCBP-CTB was found to elicit immunoprotection against a challenge with live Aeromonas cells with a relative percent survival of almost 70% and without the expression of the severe histopathological alterations induced by A. veronii.