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Digital workflows have been used to enhance the predictability of oral rehabilitations. However, techniques to digitally design and manufacture esthetic space maintainers by following a prosthetically driven treatment plan for young patients are lacking. This technique report describes a full digital workflow to produce computer-aided design and computer-aided manufacturing (CAD-CAM) esthetic space maintainers by using intraoral scans, a dental CAD software program, and a milling machine. CAD-CAM space maintainers can be provided for young patients with satisfactory patient compliance and with minimal chairside adjustment.
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PURPOSE: There is a paucity of data on the spectrum and prevalence of pathogenic variants among women of African ancestry in the Northeast region of Brazil. METHODS: We performed BROCA panel sequencing to identify inherited loss-of-function variants in breast cancer susceptibility genes among 292 Brazilian women referred to a single institution cancer risk assessment program. RESULTS: The study included a convenient cohort of 173 women with invasive breast cancer (cases) and 119 women who were cancer-free at the time of ascertainment. The majority of the women self-reported as African-descended (67% for cases and 90.8% for unaffected volunteers). Thirty-seven pathogenic variants were found in 36 (20.8%) patients. While the spectrum of pathogenic variants was heterogeneous, the majority (70.3%) of the pathogenic variants were detected in high-risk genes BRCA1, BRCA2, PALB2, and TP53. Pathogenic variants were also found in the ATM, BARD1, BRIP1, FAM175A, FANCM, NBN, and SLX4 genes in 6.4% of the affected women. Four recurrent pathogenic variants were detected in 11 patients of African ancestry. Only one unaffected woman had a pathogenic variant in the RAD51C gene. Different risk assessment models examined performed well in predicting risk of carrying germline loss-of-function variants in BRCA1 and/or BRCA2 in breast cancer cases. CONCLUSION: The high prevalence and heterogenous spectrum of pathogenic variants identified among self-reported African descendants in Northeast Brazil is consistent with studies in other African ancestry populations with a high burden of aggressive young onset breast cancer. It underscores the need to integrate comprehensive cancer risk assessment and genomic testing in the management of newly diagnosed Black women with breast cancer across the African Diaspora, enabling improved cancer control in admixed underserved and understudied populations.
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Neoplasias da Mama , Proteína BRCA1/genética , Proteína BRCA2/genética , Brasil/epidemiologia , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/epidemiologia , Neoplasias da Mama/genética , DNA Helicases/genética , Feminino , Genes BRCA2 , Predisposição Genética para Doença , Mutação em Linhagem Germinativa , Humanos , MutaçãoRESUMO
PURPOSE: The aim of this in vitro study was to assess the role of bandwidth on the area of magnetic resonance imaging (MRI) artifacts caused by orthodontic appliances composed of different alloys, using different pulse sequences in 1.5 T and 3.0 T magnetic fields. MATERIALS AND METHODS: Different phantoms containing orthodontic brackets (ceramic, ceramic bracket with a stainless-steel slot, and stainless steel) were immersed in agar gel and imaged in 1.5 T and 3.0 T MRI scanners. Pairs of gradient-echo (GE), spin-echo (SE), and ultrashort echo time (UTE) pulse sequences were used differing in bandwidth only. The area of artifacts from orthodontic devices was automatically estimated from pixel value thresholds within a region of interest (ROI). Mean values for similar pulse sequences differing in bandwidth were compared at 1.5 T and 3.0 T using analysis of variance. RESULTS: The comparison of groups revealed a significant inverse association between bandwidth values and artifact areas of the stainless-steel bracket and the self-ligating ceramic bracket with a stainless-steel slot (P<0.05). The areas of artifacts from the ceramic bracket were the smallest, but were not reduced significantly in pulse sequences with higher bandwidth values (P<0.05). Significant differences were also observed between 1.5 T and 3.0 T MRI using SE and UTE, but not using GE 2-dimensional or 3-dimensional pulse sequences. CONCLUSION: Higher receiver bandwidth might be indicated to prevent artifacts from orthodontic appliances in 1.5 T and 3.0 T MRI using SE and UTE pulse sequences.
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Introducción: en Cartagena, desde hace aproximadamente una década, se está realizando una nueva técnica de injerto dorsal denominada cartílago en puente (CEP), realizada en rinoplastias cerradas por un solo otorrinolaringólogo. No obstante, no existen estudios que caractericen las complicaciones de esta técnica. Objetivo del estudio: caracterizar las complicaciones posquirúrgicas del manejo del dorso nasal con CEP en pacientes sometidos a una rinoplastia. Metodología: estudio observacional descriptivo, de corte transversal y de carácter retrospectivo, que abarca los registros clínicos de pacientes sometidos a una rinoplastia cerrada con la técnica CEP entre 2013 y 2016. Se midieron variables sociodemográficas, características del procedimiento y se registraron las complicaciones detectadas durante el seguimiento posoperatorio: aparición de infección, extrusión, desplazamiento y reabsorción del injerto Resultados: se identificaron 882 historias clínicas. La mediana de edad fue de 28 años, siendo el 81 % del género femenino. La rinoplastia fue primaria en 80,6 %, el material del injerto dorsal fue solo cartílago en 80,6 %, Gore-Tex® en 1,7 % y una combinación de cartílago y Gore-Tex® en 17,7 %. La mediana de duración de las rinoplastias fue de 78 minutos (rango intercuartílico [RIC]: 72-85), siendo el principal procedimiento adicional la septoplastia en 82,2 %. El 18,2 % de los pacientes presentaron complicaciones. El desplazamiento fue la principal complicación reportada (14,7%), seguida de la reabsorción (4,0 %) e infección (0,1 %). Conclusiones: la técnica CEP mostró como principales complicaciones el desplazamiento y la reabsorción del injerto, con una frecuencia similar a la descrita en otros estudios.
Introduction: In Cartagena, a new dorsal graft technique called Cartilage in Bridge (CEP) has been carried out for approximately a decade, performed in rhinoplasties closed by a single otorhinolaryngologist. However, there are no studies that characterize the complications of this technique. Objective: To characterize postoperative complications of the management of the nasal dorsum with bridge cartilage in patients undergoing rhinoplasty. Methodology: Retrospective, descriptive, observational cross-sectional study, covering clinical records of patients who underwent closed rhinoplasty with the bridge cartilage technique between 2013 and 2016. Sociodemographic variables, characteristics of the procedure, and complications detected during follow-up were recorded postoperative: appearance of infection, extrusion, displacement, and reabsorption of the graft Results: 882 medical records were identified. The median age was 28 years, being 81 % female. Rhinoplasty was primary in 80.6 %, the dorsal graft material was only cartilage in 80.6 %, Gore-Tex® in 1.7 % and combination of cartilage and Gore-Tex® in 17.7 %. The median duration of the rhinoplasties was 78 minutes (ICR: 72-85), with the main additional procedure being septoplasty in 82.2 %. 18.2 % of the patients presented complications. Displacement (14.7 %) was the main complication reported, followed by reabsorption (4.0 %) and infection (0.1 %). Conclusions: The cartilage bridge technique showed graft displacement and reabsorption as main complications, with a frequency similar to that described in other studies.
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Humanos , Masculino , Feminino , Adulto , Adulto Jovem , Complicações Pós-Operatórias/epidemiologia , Rinoplastia/efeitos adversos , Cartilagens Nasais/cirurgia , Estudos Transversais , Estudos RetrospectivosRESUMO
Aim: To perform a scoped literature review on advantages of digital workflows in dentistry that could be widely adopted to address safety issues raised during the coronavirus (COVID-19) pandemic. Methods: Recent studies on any advantages of digital dentistry as compared to conventional methods that could help addressing the new safety demands for dental treatments that emerged due to the current pandemic were included. PUBMED, Embase, and Web of Knowledge databases were searched for eligible articles published in the last five years. The guidelines of PRISMA statement were followed during data extraction and evaluation. Results: The present search strategy yielded 181 publications. After application of exclusion criteria, a total of 34 studies were finally considered eligible to be discussed. Among the most important advantages of digital dentistry that contribute to safety during the current pandemic are: reduced number of clinical appointments required, shorter chairside time, less invasive surgeries and safer procedures. Conclusion: Within the limitations of this study, the findings observed herein suggest that the use of digital workflows in dentistry could lead to increased safety and reduced transmission of COVID-19 during the current pandemic
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Tecnologia Odontológica , Infecções por Coronavirus , Odontologia , Fluxo de TrabalhoRESUMO
Introducción: en Colombia, la incidencia por virus de inmunodeficiencia humana (VIH) ha ido en aumento; la ciudad de Cartagena tiene una de las más altas del país. Las manifestaciones otorrinolaringológicas en personas con VIH se estiman entre un 20%-80%, lo que genera un gran impacto en la calidad de vida. Objetivo: determinar las características epidemiológicas y las manifestaciones otorrinolaringológicas en un grupo de personas con VIH/Sida de la ciudad de Cartagena. Metodología: diseño observacional descriptivo de corte transversal y prospectivo. Se recolectó información de pacientes con VIH de la ciudad de Cartagena, que asistieron a dos centros médicos. Se les aplicó un cuestionario para obtener datos epidemiológicos, signos y síntomas otorrinolaringológicos, así como un examen físico otorrinolaringológico completo. Resultados: se incluyeron 150 pacientes en el estudio, con una media de edad de 31 años, 59,3% del género masculino y 40,7% del femenino. El antecedente patológico no otorrinolaringológico más frecuente fue la coinfección por sífilis en un 10%; el otorrinolaringológico fue la sinusitis y la candidiasis oral, cada uno con un 3,3%. El 73% de los pacientes manifestó alteración otorrinolaringológica en el momento de la evaluación. Las más frecuentes fueron las otológicas, con el 39,9% de los pacientes. Además, se observó una relación estadísticamente significativa entre los conteos de CD4 y hallazgos como disfonía en laringe (p = 0,045). Conclusiones: las manifestaciones otológicas fueron las más frecuentes en nuestro medio y se encontró una relación entre el conteo de CD4 y las manifestaciones laríngeas de la enfermedad.
Introduction: In Colombia, the incidence of the disease has been increasing and Cartagena has the highest numbers of the country. Otorhinolaryngological manifestations in people with HIV/AIDS are estimated between 20%-80% generating a great impact on quality of life. Objective: To determine the epidemiological characteristics and otorhinolaryngological manifestations in a group of people with HIV / AIDS in the city of Cartagena. Methodology: Observational, descriptive, cross-sectional and prospective design. Information was collected from patients with HIV from the city of Cartagena who attended 2 medical centers, a questionnaire was applied to obtain epidemiological data, otorhinolaryngological signs and symptoms, as well as a complete otorhinolaryngological physical examination. Results: 150 patients were included in the study, with a mean age of 31 years, 59.3% male and 40.7% female. The most frequent non-otorhinolaryngological pathological antecedent was syphilis coinfection in 10% and otorhinolaryngological, sinusitis and oral candidiasis each with 3.3%. 73% of the patients at the time of the evaluation manifested otorhinolaryngological alteration, the otological ones being the most frequent with 39.9% of the patients. Furthermore, a significant relationship was observed between CD4 counts and findings such as laryngeal dysphonia (p = 0.045). Conclusions: The otological manifestations were the most frequent in our environment and a relationship was found between the CD4 count and the laryngeal manifestations of the disease.
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Humanos , HIV , Otorrinolaringopatias , Linfócitos T CD4-Positivos , Síndrome da Imunodeficiência AdquiridaRESUMO
Although the discipline of cell biology (CB) is part of the curricula of predoctoral dental schools, students often fail to recognize its practical relevance. The aim of this study was to assess the effectiveness of a practical-theoretical project-based course in closing the gaps among CB, scientific research, and dentistry for dental students. A project-based learning course was developed with nine sequential lessons to evaluate 108 undergraduate dental students enrolled in CB classes of a Brazilian school of dentistry during 2013-16. To highlight the relevance of in vitro studies in the preclinical evaluation of dental materials at the cellular level, the students were challenged to complete the process of drafting a protocol and performing a cytocompatibility assay for a bone substitute used in dentistry. Class activities included small group discussions, scientific database search and article presentations, protocol development, lab experimentation, and writing of a final scientific report. A control group of 31 students attended only one laboratory class on the same theme, and the final reports were compared between the two groups. The results showed that the project-based learning students had superior outcomes in acknowledging the relevance of in vitro methods during biocompatibility testing. Moreover, they produced scientifically sound reports with more content on methodological issues, the relationship with dentistry, and the scientific literature than the control group (p<0.05). The project-based learning students also recognized a higher relevance of scientific research and CB to dental practice. These results suggest that a project-based approach can help contextualize scientific research in dental curricula.
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Biologia Celular/educação , Educação em Odontologia/métodos , Ensino , Currículo , HumanosRESUMO
The application of in silico methods is increasing on toxicological risk prediction for human and environmental health. This work aimed to evaluate the performance of three in silico freeware models (OSIRIS v.2.0, LAZAR, and Toxtree) on the prediction of carcinogenicity and mutagenicity of thirty-eight volatile organic compounds (VOC) related to chemical risk assessment for occupational exposure. Theoretical data were compared with assessments available in international databases. Confusion matrices and ROC curves were used to evaluate the sensitivity, specificity, and accuracy of each model. All three models (OSIRIS, LAZAR and Toxtree) were able to identify VOC with a potential carcinogenicity or mutagenicity risk for humans, however presenting differences concerning the specificity, sensitivity, and accuracy. The best predictive performances were found for OSIRIS and LAZAR for carcinogenicity and OSIRIS for mutagenicity, as these softwares presented a combination of negative predictive power and lower risk of false positives (high specificity) for those endpoints. The heterogeneity of results found with different softwares reinforce the importance of using a combination of in silico models to occupational toxicological risk assessment.
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Carcinógenos/toxicidade , Testes de Mutagenicidade/métodos , Mutagênicos/toxicidade , Medição de Risco/métodos , Compostos Orgânicos Voláteis/toxicidade , Simulação por Computador , Bases de Dados Factuais , Humanos , Modelos Biológicos , Mutagênese/efeitos dos fármacos , Exposição Ocupacional/efeitos adversos , Sensibilidade e Especificidade , SoftwareRESUMO
One of the most common oral surgical procedures is the extraction of the lower third molar (LTM). Postoperative complications such as paresthesia due to inferior alveolar nerve (IAN) injury are commonly observed in cases of horizontal and vertical impaction. The present report discusses a case of a vertically impacted LTM associated with a dentigerous cyst. An intimate contact between the LTM roots and the mandibular canal was observed on a panoramic radiograph and confirmed with cone-beam computed tomographic (CBCT) cross-sectional cuts. An orthodontic miniscrew was then used to extrude the LTM prior to its surgical removal in order to avoid the risk of inferior alveolar nerve injury. CBCT imaging follow-up confirmed the success of the LTM orthodontic extrusion.
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The present retrospective case series is aimed at evaluating a staged approach using a removable partial denture (RPD) as an interim prosthesis in treatment to correct a failing dentition until such time as a full-arch fixed implant-supported prosthesis may be inserted. Eight patients, who had undergone maxillary full-arch rehabilitation with dental implants due to poor prognosis of their dentitions, were analyzed. All treatment included initial periodontal therapy and a strategic order of extraction of hopeless teeth. An RPD supported by selected teeth rehabilitated the compromised arch during implant osseointegration. These remaining teeth were extracted prior to definitive prosthesis delivery. Advantages and drawbacks of this technique were also recorded for the cases presented. Among the advantages provided by the staged approach are simplicity of fabrication, low cost, and ease of insertion. Additionally, RPD tooth support prevented contact between the interim prosthesis and healing abutments, promoting implant osseointegration. The main drawbacks were interference with speech and limited esthetic results. Implant survival rate was 100% within a follow-up of at least 1 year. The use of RPDs as interim prostheses allowed for the accomplishment of the analyzed rehabilitation treatments. It is a simple treatment alternative for patients with a low smile line.