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Since the discovery of the third allotropic carbon form, carbon-based one-dimensional nanomaterials (1D-CNMs) became an attractive and new technology with different applications that range from electronics to biomedical and environmental technologies. Despite their broad application, data on environmental risks remain limited. Fish are widely used in ecotoxicological studies and biomonitoring programs. Thus, the aim of the current study was to summarize and critically analyze the literature focused on investigating the bioaccumulation and ecotoxicological impacts of 1D-CNMs (carbon nanotubes and nanofibers) on different fish species. In total, 93 articles were summarized and analyzed by taking into consideration the following aspects: bioaccumulation, trophic transfer, genotoxicity, mutagenicity, organ-specific toxicity, oxidative stress, neurotoxicity and behavioral changes. Results have evidenced that the analyzed studies were mainly carried out with multi-walled carbon nanotubes, which were followed by single-walled nanotubes and nanofibers. Zebrafish (Danio rerio) was the main fish species used as model system. CNMs' ecotoxicity in fish depends on their physicochemical features, functionalization, experimental design (e.g. exposure time, concentration, exposure type), as well as on fish species and developmental stage. CNMs' action mechanism and toxicity in fish are associated with oxidative stress, genotoxicity, hepatotoxicity and cardiotoxicity. Overall, fish are a suitable model system to assess the ecotoxicity of, and the environmental risk posed by, CNMs.

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The pandemic has affected people's mental health and university students are considered one of the most vulnerable groups, encouraging the development of psychological interventions that can minimize the suffering and illness of this public. Among the possibilities of intervention, virtual clinical listening groups were created which, during the period of social isolation, had the purpose of opening up a listening space for university students suffering from emotional distress. The focus of the group meetings was how university students were experiencing the pandemic, as well as the consequences of this experience. In this article, we discuss virtual clinical listening groups as a form of psychological intervention in the mental health care of university students during the COVID-19 pandemic at a university in northeastern Brazil. This is a qualitative study that began with 274 university students and 5 psychologists who facilitated the listening groups. We used as instruments the registration forms, the participation records of the virtual clinical listening groups and the field diaries written by the facilitators after each meeting. We described in the results the collected data and, from the field diaries, the themes that emerged in the various meetings were listed and analyzed in dialogue with the literature. At the end of the psychological screening process, 117 students participated in the 17 organized groups, with an average of 8 university students per group. Among the main motivations for participating in virtual clinical listening groups, we highlight: the desire to share experiences, a search for control of anxiety, depression and stress, care for mental health, a willingness to promote listening to psychological support for themselves and for others, and self-knowledge. We discuss the nuances in the process of forming the listening groups and the characteristics of the participants, as well as a central element of the group process, which is the establishment of bonds and mutual help among the participants. Feeling vulnerable and the fear of contamination is an element of suffering and, above all, of stress experienced by university students. The group presented itself as an alternative of mutual care in the pandemic context.

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Zebrafish (Danio rerio) is a tropical fish species widely used in research, worldwide. The development of genetically modified animals and the increasing number of zebrafish breeding facilities due to their emerging use in several research fields, opened room for new ethical challenges for research carried out with this species. It is necessary to raise the scientific community's awareness of the ethical standards and laws in force, on animal research. Thus, the aim of the current study is to describe 10 Rs ethical principles by using zebrafish as model system in research. The classical 3 Rs concerning animal welfare, namely replacement, reduction and refinement; and the added 7 Rs related to scientific (registration, reporting, robustness, reproducibility and relevance) and conduct principles (responsibility, and respect) in zebrafish research are herein presented and critically discussed. The use of these 10 Rs by researchers, institutions and the Animal Ethics Committee is recommended to support regulations, decision-making about and the promotion of zebrafish health and welfare in research.

O peixe-zebra (Danio rerio) é um peixe tropical amplamente usado em pesquisas em todo o mundo. Devido ao seu uso emergente em várias áreas de pesquisa, o desenvolvimento de animais geneticamente modificados e o aumento das instalações de peixe-zebra, novos desafos éticos surgem na pesquisa com o peixe-zebra. Além disso, é necessária a conscientização da comunidade científica sobre as normas éticas e leis vigentes na pesquisa com animais. Assim, o presente estudo teve como objetivo descrever os princípios éticos de 10 Rs usando o peixe-zebra como sistema modelo em pesquisa. Os 3 Rs clássicos relativos ao bem-estar animal (substituição, redução e refinamento) e 7 Rs adicionais relacionados aos princípios científicos (registro, relatório, robustez, reprodutibilidade e relevância) e de conduta (responsabilidade e respeito) na pesquisa do peixe-zebra são apresentados e discutido criticamente. Recomendamos o uso desses 10 Rs pelos pesquisadores, instituições e Comitê de Ética Animal na regulamentação, decisão e promoção da saúde e bem-estar do peixe-zebra em pesquisas.

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Compostos fenólicos e capacidade antioxidante são mecanismos de defesa das plantas aos danos do estresse oxidativo. Os compostos fenólicos são sintetizados pela via dos fenilpropanoides, cuja enzima chave, fenilalanina amônia liase, é influenciada pela luz e ação de fotorreceptores, como o fitocromo. O objetivo do presente trabalho é avaliar a concentração de compostos fenólicos e a capacidade antioxidante de frutos de microtomateiro selvagem, cultivar "Micro-Tom" (MT), e seus mutantes fotomorfogenéticos high pigment 1 (hp1), super-responsivo a eventos mediados por luz e aurea (au), deficiente quantitativo em fitocromos. Vinte frutos maduros de cada genótipo (MT, hp1 e au) foram utilizados para as análises, realizadas em triplicata. Para quantificação dos compostos fenólicos totais, foi utilizado o método de Folin-Ciocalteu e a capacidade antioxidante foi realizada pelos métodos Ferric Reducing Antioxidant Power (FRAP) e 2,2-diphenyl-1-picrylhydrazyl (DPPH). Os frutos do mutante hp1 apresentaram maiores conteúdos de compostos fenólicos totais e também maior capacidade antioxidante em relação à cultivar selvagem ("MT") e ao mutante au, o qual não diferiu significativamente da cultivar "MT".(AU)

Phenolic compounds and antioxidant capacity are defense mechanisms of plants against the oxidative stress damage. Phenolic compounds are synthesized through the phenylpropanoid pathway, where the enzyme phenylalanine-ammonia-lyase plays a key role and it is influenced by light and photoreceptors such as phytochromes. The present research aims to evaluate the phenolic compounds content and antioxidant capacity of the wild "Micro-Tom" (MT) cultivar tomato fruits and its photomorphogenic mutant tomato plants high pigment 1 (hp1), super responsive to events mediated by light, and aurea (au), quantitative phytochrome deficient. Twenty mature fruits of each genotype ("MT", hp1, au) were used in triplicate for analyses. To quantify the total phenolic compounds the Folin-Ciocalteu method was used and the antioxidant capacity was analyzed by Ferric Reducing Antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods. The hp1 mutant presented the highest total phenolic compounds content and higher antioxidant capacity than wild cultivar ("MT") and au mutant, which did not differ significantly from "MT" cultivar.(AU)

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RESUMO

Compostos fenólicos e capacidade antioxidante são mecanismos de defesa das plantas aos danos do estresse oxidativo. Os compostos fenólicos são sintetizados pela via dos fenilpropanoides, cuja enzima chave, fenilalanina amônia liase, é influenciada pela luz e ação de fotorreceptores, como o fitocromo. O objetivo do presente trabalho é avaliar a concentração de compostos fenólicos e a capacidade antioxidante de frutos de microtomateiro selvagem, cultivar "Micro-Tom" (MT), e seus mutantes fotomorfogenéticos high pigment 1 (hp1), super-responsivo a eventos mediados por luz e aurea (au), deficiente quantitativo em fitocromos. Vinte frutos maduros de cada genótipo (MT, hp1 e au) foram utilizados para as análises, realizadas em triplicata. Para quantificação dos compostos fenólicos totais, foi utilizado o método de Folin-Ciocalteu e a capacidade antioxidante foi realizada pelos métodos Ferric Reducing Antioxidant Power (FRAP) e 2,2-diphenyl-1-picrylhydrazyl (DPPH). Os frutos do mutante hp1 apresentaram maiores conteúdos de compostos fenólicos totais e também maior capacidade antioxidante em relação à cultivar selvagem ("MT") e ao mutante au, o qual não diferiu significativamente da cultivar "MT".

Phenolic compounds and antioxidant capacity are defense mechanisms of plants against the oxidative stress damage. Phenolic compounds are synthesized through the phenylpropanoid pathway, where the enzyme phenylalanine-ammonia-lyase plays a key role and it is influenced by light and photoreceptors such as phytochromes. The present research aims to evaluate the phenolic compounds content and antioxidant capacity of the wild "Micro-Tom" (MT) cultivar tomato fruits and its photomorphogenic mutant tomato plants high pigment 1 (hp1), super responsive to events mediated by light, and aurea (au), quantitative phytochrome deficient. Twenty mature fruits of each genotype ("MT", hp1, au) were used in triplicate for analyses. To quantify the total phenolic compounds the Folin-Ciocalteu method was used and the antioxidant capacity was analyzed by Ferric Reducing Antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods. The hp1 mutant presented the highest total phenolic compounds content and higher antioxidant capacity than wild cultivar ("MT") and au mutant, which did not differ significantly from "MT" cultivar.

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Durante o transcorrer do nosso aprimoramento científico, pessoal e profissional, no curso de Doutorado fomos contempladas pela Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), por meio de concurso nacional, com um Estágio de Doutorando (Sanduíche) na cidade do Porto, em Portugal. O desenvolvimento do Projeto O Cuidado Humanístico de Enfermagem ao Recém-Nascido na Unidade de Internação Neonatal: experiência de intercâmbio em Portugal, ocorreu no período de setembro a dezembro de 2005 e constou de uma observação participante junto a 10 enfermeiros de Unidades de Cuidados Intensivos Neonatais, atuantes em duas instituições hospitalares da cidade do Porto, quanto aos cuidados ao recém-nascido, sob a perspectiva humanística à luz da Teoria Humanística de Enfermagem, de Paterson e Zderad (1976). Ressaltamos a importância deste intercâmbio para a ampliação da Enfermagem Neonatal e o crescimento humano e profissional dos enfermeiros como capazes de repensar o fazer e o ser e participar, com todos os envolvidos, de uma nova maneira de cuidar de forma humanizada em Enfermagem, na pesquisa e no ensino.

During the course of our scientific improvement, personal and professional development in the Doctorate Course we were contemplated by the Coordination of Postgraduate Personnel Improvement (CAPES), through a national contest, with a (Sandwich) Doctorate Period of Training in the city of Porto, Portugal. The development of the project Nursing Humanistic Care to the Newborn in the Neonatal Care Unit: interchange experience in Portugal, was held in the period from September through December 2005 and was composed of a participant observation with 10 nurses from Neonatal Intensive Care Units, acting in two hospitals of Porto, about the care to newborns, on the humanistic perspective in the light of the Nursing Humanistic Theory, of Paterson and Zderad (1976). We highlight the importance of this interchange for the enhancement of the Neonatal Nursing and the human and professional growth of the nurses as able to rethink the do and the be and participate, with all the people involved, of a new way to take care in a humanized way in Nursing, in the research and in the teaching.

Durante el transcurrir de nuestro aprimoramiento científico, personal y además profesional, en el curso de Doctorado fuimos contemplados por la Coordinação de Perfeccionamiento de Personal de Nivel Superior (CAPES), a través de concurso nacional, con un Etapa del Doctorado en una otra institución, en la ciudad del Porto, en Portugal. El desarrollo del Proyecto La Atención Humanística de Enfermería al Recién Nacido en la Unidad de Internación Neonatal: experiencia de intercambio en Portugal, ocurrió en el período de septiembre a diciembre de 2005 y constó de una observación participante junto a 10 enfermeros de Unidades de Atenciones Intensivas Neonatales, actuantes en dos instituciones hospitalares de la ciudad de Porto, mientras a las atenciones al recién nacido, bajo la perspectiva humanística a la luz de la Teoría Humanística de Enfermería, de Paterson y Zderad (1976). Así, resaltamos la importancia de este intercambio para la ampliación de la Enfermería Neonatal y el crescimiento humano y profesional de los enfermeros como capaces de pensar una vez más lo hacer y lo ser y además participar con todos los envueltos de una nueva manera de cuidar de forma humanizada en Enfermería, ahora en la investigación y en la enseñanza.

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