RESUMO
Biliary neoplasms are uncommon in cats and affect older animals. A 12-year-old female crossbreed cat showed prostration, lethargy, apathy, and severe jaundice. Ultrasonography showed distention of the gallbladder associated with severe obstruction of the bile ducts with thickening of the biliary wall, forming amorphous masses of irregular contour and heterogeneous appearance directed to the lumen measuring up to 2 cm. Necropsy showed a gallbladder with a yellowish and soft nodule measuring 3 × 3 cm, compressing the extrahepatic bile duct, occluding the passage of bile. There were also firm, yellowish multifocal to coalescing nodules in the liver, ranging from 0.5 to 1 cm, affecting 10% of the organ, in addition to lungs with firm, yellowish multifocal nodules ranging from 0.2 to 0.5 cm, affecting 20% of the organ. Histologically, gallbladder and bile ducts had malignant epithelial neoplastic proliferation, which was organized into multiple papillary and ductal projections, separated by moderate fibrovascular stroma compatible with gallbladder adenocarcinoma. The liver and lungs also contained neoplastic structures with a ductal appearance and papilliform projections identical to those observed in the gallbladder. The immunohistochemical examination (IHC) showed intense positive staining of epithelial neoplastic cells for pan-cytokeratin (AE1/AE3) and no staining for vimentin (Clone V9). The diagnosis of gallbladder adenocarcinoma with metastasis in the liver and lungs was established based on the clinical, macroscopic, histopathological, and immunohistochemical findings.(AU)
Assuntos
Animais , Feminino , Adenocarcinoma/diagnóstico , Gatos , Metástase Neoplásica/diagnóstico , Vesícula Biliar/fisiopatologiaRESUMO
This report describes the clinical features and molecular diagnosis of a case of canine leproid granuloma (CLG) caused by mycobacterial strains of the Mycobacterium simiae complex in Brazil. A 12-year-old non-neutered male Labrador Retriever dog was presented with a 2-week history of progressive painless cutaneous lesions. Ulcerated nodules with hematic crusts were observed on the dorsal surface of the right and left pinna and on the metacarpal, metatarsal, and digits. Complete blood count, serum biochemistry, aspiration cytology of cutaneous lesions, biopsy for histopathological evaluation, culture for aerobic and anaerobic bacteria, polymerase chain reaction and DNA sequencing to identify mycobacterial species were performed. According to the clinical and histopathological findings, a diagnosis of CLG was established. Despite the negative result of the bacterial culture, mycobacterial identification was made by sequencing the hsp65 gene. Our findings highlight that mycobacterial species closely related to members of the M simiae clade can be causative agents of CLG.
Assuntos
Doenças do Cão , Infecções por Mycobacterium , Mycobacterium , Animais , Brasil , Doenças do Cão/patologia , Cães , Granuloma/microbiologia , Granuloma/patologia , Granuloma/veterinária , Masculino , Mycobacterium/genética , Infecções por Mycobacterium/diagnóstico , Infecções por Mycobacterium/veterináriaRESUMO
This report aims to describe one case of plasma cell pododermatitis associated with feline leukemia virus (FeLV) and concomitant feline immunodeficiency virus (FIV) infection in a cat. A 2-year-old, intact male, mixed-breed cat was presented with alopecia, skin peeling, and erythematous swelling in the left metacarpal paw pad. Swelling, softening, ulceration with secondary crusts, and erythematous to violaceous discoloration were observed in multiple metacarpal, metatarsal, and digital paw pads. Complete blood count and serum biochemistry were analyzed. FeLV antigenemia and FIV seropositivity were assessed by immunoassay (enzyme-linked immunosorbent assay). Nested-PCR was used to detect FIV and FeLV proviral DNA in blood cells. Histopathological examination and anti-FeLV and anti-FIV immunohistochemical were performed on paw pad biopsies. According to clinical and histopathological findings, a diagnosis of plasma cell pododermatitis was made. The cat was FIV and FeLV seropositive. The immunohistochemical of paw pad biopsies revealed FeLV positivity and FIV negativity. This study provides reference for further investigations about feline plasma cell pododermatitis and highlights retrovirus infection as a potential factor associated with this disease.