RESUMO
This study investigates the effects of an ethanolic extract from the stem bark of Combretum leprosum Mart. & Eiche (Combretaceae) on experimental ulcers induced by ethanol and indomethacin and on gastric secretion and mucus content in pylorus-ligated rats. The effects were compared with those of ranitidine and carbenoxolone. Combretum leprosum orally administered elicited a complete inhibition of the appearance of gastric lesions induced by ethanol and a partial reduction when indomethacin was used as an ulcerogenic agent. Moreover, the protection against gastric ulceration induced by ethanol was decreased with indomethacin pretreatment. The intraduodenal administration of Combretum leprosum in four-hour pylorus-ligated rats increased the volume and pH of gastric juice while decreasing the acid output and produced a significant increase in gastric wall mucus content. The major compounds detected in a preliminary phytochemical screening were triterpenes, flavonoids, taninns and saponins. This study provides evidence that the ethanolic extract of Combretum leprosum possesses gastroprotective and anti-ulcerogenic effects, which are related to the inhibition of the gastric acid secretion and an increase of mucosal defensive factors such as mucus and prostaglandin.
Assuntos
Antiulcerosos , Combretum/química , Úlcera Gástrica/induzido quimicamente , Úlcera Gástrica/prevenção & controle , Animais , Anti-Inflamatórios não Esteroides , Etanol , Ácido Gástrico/metabolismo , Mucosa Gástrica/efeitos dos fármacos , Mucosa Gástrica/metabolismo , Indometacina , Masculino , Muco/metabolismo , Casca de Planta/química , Extratos Vegetais , Caules de Planta/química , Ratos , Ratos Wistar , SolventesRESUMO
Carbachol-induced contractions of rat stomach fundus strips, obtained in a nutrient solution containing 1.8 mM Ca2+, were resistant to Ca2+ withdrawal, even after 1 h of bathing the tissues in a nominal 0 Ca2+ solution. This was not observed when K+ was used to evoke contractions, which were rapidly inhibited after Ca2+ removal (t1/2=2 min). The effect of carbachol in 0 Ca2+ solution was reduced by using drugs that reduce intracellular pools of Ca2+, such as caffeine (1-3 mM), ryanodine (30 microM) or thapsigargin (1 microM), corroborating the involvement of intracellular Ca2+ stores. On the other hand, when the 0 Ca2+ solution contained EGTA, a complete decline of carbachol effects was observed within about 8 min, indicating the involvement of extracellular Ca2+. Atomic absorption spectrometry showed that our 0 Ca2+ solution still contained 45 microM Ca2+, which was drastically reduced to 5.9 nM in the presence of EGTA. Taken together, our results indicate that the effects of carbachol are due to the mobilization of caffeine-, ryanodine- and thapsigargin-sensitive intracellular Ca2+ stores, and that these stores are not inactivated or depleted if micromolar concentrations (45 microM), but not nanomolar concentrations (5.9 nM) of Ca2+ are maintained in the extracellular milieu.
Assuntos
Cálcio/metabolismo , Fundo Gástrico/fisiologia , Receptores Muscarínicos/metabolismo , Animais , Transporte Biológico/efeitos dos fármacos , Cafeína/farmacologia , Cálcio/farmacologia , Carbacol/farmacologia , Quelantes/farmacologia , Agonistas Colinérgicos/farmacologia , Ácido Egtázico/farmacologia , Inibidores Enzimáticos/farmacologia , Feminino , Músculo Liso/fisiologia , Níquel/farmacologia , Peristaltismo/efeitos dos fármacos , Inibidores de Fosfodiesterase/farmacologia , Ratos , Ratos Endogâmicos WF , Rianodina/farmacologia , Tapsigargina/farmacologiaRESUMO
Relaxation induced by NANC-nerve stimulation is reduced by nitric oxide synthase (NOS) inhibitors but not by superoxide generators or NO scavengers, casting doubts on the precise nature of the neurotransmitter being released by these nerves. The lack of effect of superoxide anion generators to inhibit nitrergic nerve-mediated relaxations has been attributed to the protective action of high tissue levels of superoxide dismutase (SOD). The effects of hydroquinone, hydroxocobalamin and carboxy-PTIO, three NO inactivators which do not depend on superoxide anion generation, upon nitrergic nerve-mediated relaxations of the rat proximal duodenum were determined in order to elucidate whether they are mediated by free NO. GABA and nicotine caused relaxations of isolated segments of the rat proximal duodenum in a concentration-dependent manner that were abolished by tetrodotoxin (TTX). Similarly, transmural electrical stimulation (TES) caused frequency-dependent relaxations that were also abolished by TTX. The NOS inhibitors L-NAME and L-NOARG reduced in a concentration-dependent manner nerve-mediated relaxations elicited by TES, nicotine and GABA. The effect of NOS inhibitors was prevented by L-arginine but not D-arginine. NO caused concentration-dependent relaxations that were not affected by TTX or L-NOARG but were abolished by hydroquinone, hydroxocobalamin and carboxy-PTIO. In contrast, these compounds failed to affect TES-, nicotine- and GABA-induced relaxations. The lack of effect of hydroquinone, hydroxocobalamin and carboxy-PTIO upon nerve-mediated relaxations was unaltered by pretreatment with the SOD irreversible inhibitor DETCA. The present findings show that nitrergic nerve-mediated relaxations of the rat duodenum are unaffected by NO inactivators that do not generate superoxide anion. It is suggested that either a NO-containing molecule that is unreactive with the inactivators tested is the inhibitory neurotransmitter released by nitrergic nerves or that NOS activity fulfills another role in nitrergic nerves which could be related to the release of an still unidentified transmitter.
Assuntos
Sistema Nervoso Autônomo/efeitos dos fármacos , Benzoatos/farmacologia , Duodeno/efeitos dos fármacos , Duodeno/inervação , Hematínicos/farmacologia , Hidroquinonas/farmacologia , Hidroxocobalamina/farmacologia , Imidazóis/farmacologia , Músculo Liso/efeitos dos fármacos , Músculo Liso/inervação , Animais , Estimulação Elétrica , Masculino , Relaxamento Muscular/efeitos dos fármacos , NG-Nitroarginina Metil Éster/farmacologia , Nitroarginina/farmacologia , Ratos , Ratos Wistar , Ácido gama-Aminobutírico/farmacologiaRESUMO
In rat stomach fundus, contractions induced by Ca2+ (1.8 mM) were strikingly potentiated by thapsigargin. This potentiation was partially inhibited by the blockers of Ca2+ release activated channels (CRACs), miconazole and SK&F96365 ([1-[beta-[3-(4-methoxyphenyl)propoxy]-4-methoxyphenethyl]-1H-imidazole, HCL]) and slightly blocked by the antagonist of calcium voltage-operated channels (VOCs), isradipine. In dissociated cells in a 0Ca solution, thapsigargin potentiated the increase in intracellular calcium after reintroduction of Ca2+. This potentiation was partially reduced by the CRAC blockers, but not by the VOC blockers. This data suggests that calcium influx increased due to the depletion of intracellular calcium by thapsigargin and that this influx occurs predominantly through CRACs.