RESUMO
AIMS: Fructooligosaccharides (FOSs) known for their health properties and ß-(2â6)-levan-type FOSs have shown prebiotic and immunomodulatory activities that overcome those of commercial ß-(2â1)-FOSs, but costs do not favour their use. Moreover, FOSs can reach the bloodstream through the diet, and little is known about their direct effect on cells. The aim of this work was to produce high-content FOSs by Bacillus subtilis natto CCT7712 in a bioreactor using commercial sucrose and to evaluate their antiproliferative effects in OVCAR-3 cells. METHODS AND RESULTS: FOS production reached 173·60 g l-1 , 0·2 vvm aeration and uncontrolled pH. Levan-type FOSs, composed of ß-(2 â 6) links and mainly GF3 (6-nystose), were identified using RMN spectroscopy, FT-IR and ESI-MS. FOSs decreased the viability and proliferation of OVCAR-3 cells, and the effects were associated with an increased pro-inflammatory response by the induction of IL-8 and TNF-α, and the repression of ER-ß genes. The metabolic profiles showed disruption of cellular homeostasis that can be associated with a decrease in proliferation. CONCLUSIONS: The high production of levan-type FOSs from B. subtilis natto CCT7712 in a bioreactor was achieved, and they showed antiproliferative potential in OVCAR-3 cells. SIGNIFICANCE AND IMPACT OF THE STUDY: FOS could be a good target for future therapeutic studies and commercial use.
Assuntos
Bacillus subtilis/metabolismo , Proliferação de Células/efeitos dos fármacos , Oligossacarídeos/metabolismo , Oligossacarídeos/farmacologia , Reatores Biológicos , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Frutanos/química , Frutanos/metabolismo , Frutanos/farmacologia , Humanos , Oligossacarídeos/química , Sacarose/metabolismoRESUMO
Fermentations utilizing strains of Zymomonas mobilis, in place of the traditional yeasts, have been proposed due their ethanol yields being close to theoretical. Ethanol production from sugar cane molasses was analyzed under different culture conditions using Z. mobilis in batch fermentation. The total reducing sugars (TRS) concentrations in the molasses, temperature, agitation and culture time effects were studied simultaneously through factorial design. The best conditions for ethanol production were 200 g L(-1) of total reducing sugars in the molasses, temperature of 30 degrees C and static culture and time of fermentation of 48 h, achieving 55.8 g L(-1). The pH of the medium was kept constant during the experiments, showing that molasses presents a buffering effect.
Assuntos
Carboidratos , Fermentação , Melaço , Temperatura , Zymomonas/metabolismoRESUMO
The possibility of using fat as food and fuel and the production of specific compounds from lipids has led research about micro-organisms capable of conversion carbohydrate into lipids. The microorganisms: Candida parakrusei, Hansenula suaveloens and an unidentified yeast "yeast", isolated from sugar-alcohol sector have been studied for lipid productions utilising sugar-cane molasses and molasses supplemented with sodium chloride and calcium panthotenate. This factors stimulated the lipids total productions to the three micro-organisms. The major values was 60,26 per cent; 53,97 per cent and 58,46 per cent to C, parakrusei in NaCl at 0,1 N, H. suaveloens in calcium panthotenate 4ug/mL and yeast "a" in calcium panthotenate 16ug/mL respectively. Furthermore strain "a" achieved the highest values of phospolipids while strain C parakrusei the higest values of sterol and protein. The fatty acid analysis of strains showed predominance of oleic palmitic and linoleic acid
Assuntos
Bioquímica , Ácidos Graxos , Ácido Linoleico , Lipídeos , Pichia , Álcoois AçúcaresRESUMO
The effect of soya bean oil deodorizing (SODD) on sugar cane juice fermentation in batch culture by zymomonas mobilis CP4 and Saccharomyces cerevisiae has beens investigated. At 15 per cent (w/v) total reducing sugars and no SODD added, the sugars were not fully metabolized as the results for Zymomonas and Saccharomyces were respectively: conversion efficiency 22.4 per cent and 12,2 per cent; maximum specific groth rate 0,20 h-1 and 0,21 h-1 whereas the biomass formed by the bacteria was about half that formed by the yeast as growth yield was 0,011 for Zymomonas and 0,026 for Saccharomyces. However, when cane juice was added with SODD 0,01 per cent(v/v) the sugars were more efficiency utilised as the results for Zymomonas and Saccharomyces raised respectively: 41,2 per cent and 17,6 per cent which are 84 per cent and 45 per cent higher as cane juice was used without SODD; 0,19 per cent and 0,21 per cent h-1, and Yx/s for zymomonas lowered to 0,008 and Saccharomyces increased to 0,044(AB)