RESUMO

Amoebae were isolated from a natural thermal water source in Michoacán, Mexico, in September 1986. Two 500-ml samples were taken from pools with water at 45 degrees C and 46 degrees C and concentrated at 2,000 g for 15 min. The sediment was seeded on nonnutritive agar plates and incubated at 42 degrees C. The isolates were axenized in bactocasitone-serum medium. The identification of the isolates was based on their morphology, total protein and isoenzyme patterns by agarose isoelectric focusing, serology, fine structure, agglutination with Concanavalin A, sensitivity to trimethoprim, capacity to kill mice, and their cytopathic effect in Vero cells. The results showed several morphophysiological, biochemical and serological differences between the isolates and the type strain Aq/9/1/45D of Naegleria lovaniensis. These remarkable differences provide sufficient evidence to consider one of the isolates a new subspecies, and the other one a morphological variant of N. l. lovaniensis, which can be differentiated from other Naegleriae by their morphology, biochemistry, serology and physiology. The authors propose the name tarasca for the subspecies and purepecha for the morphological variant.

Assuntos

Naegleria/classificação , Amebíase/patologia , Animais , Eletroforese/métodos , Flagelos , Imunofluorescência , Imunodifusão , México , Camundongos , Naegleria/efeitos dos fármacos , Naegleria/isolamento & purificação , Naegleria/patogenicidade , Naegleria/ultraestrutura , Especificidade da Espécie , Trimetoprima/farmacologia , Células Vero

Assuntos

Larva Migrans Visceral/diagnóstico , Adolescente , Criança , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Feminino , Testes Hematológicos , Humanos , Lactente , Larva Migrans Visceral/tratamento farmacológico , Masculino , Tiabendazol/uso terapêutico , Toxocara/imunologia

RESUMO

Se estudiaron clinicamente y mediante examenes de laboratorio 42 ninos infectados por Toxocara sp. La edad promedio de los casos fue de 4 anos 2 meses +/- 3 anos 5 meses. Habia geofagia en 97,6%, grados variables de compromiso pulmonar en 64,3% y hepatomegalia en 59,5% de los pacientes.El fondo de ojos tenia alteraciones en 4,8% de los pacientes. En el hemograma la concentracion de hemoglobina promedio fue de 11,0 g/dl, el recuento de leucocitos promedio 19.213 x mm3 y el de eosinofilos 8.918,9 x mm3. 84,86% de los ninos presentaron niveles de isohemaglutininas > o igual 1:64 y 95,2% titulos de ELISA para Toxocara superiores a esta dilucion. El tratamiento con tiabendazol fue bien tolerado en 83,3% y efectivo en 70,8% de los ninos. En 29,2% se observo mejoria parcial clinica y de laboratorio

Assuntos

Lactente , Pré-Escolar , Criança , Adolescente , Humanos , Masculino , Feminino , Larva Migrans Visceral , Ensaio de Imunoadsorção Enzimática