RESUMO
BACKGROUND: Hospital reservoirs of Achromobacter xylosoxidans, responsible for nosocomial infections, are poorly known. METHODS: We examined the growth, survival and biofilm formation of five A. xylosoxidans strains for up to 2 y in distilled, dialysis or microfiltered water. Each strain was inoculated at 102 CFU/ml without adding nutrients. RESULTS: All strains grew at a level of 3x103 to 1.5x107 CFU/ml; each strain showed a preferred water type. Strains isolated from quaternary ammoniums showed the highest ability to grow and form biofilms in nutrient-poor waters. CONCLUSION: Medical waters and notably sterile distilled water bottles appear to be long-lasting reservoirs of A. xylosoxidans.
Assuntos
Achromobacter denitrificans , Biofilmes , Hospitais , HumanosRESUMO
BACKGROUND: Achromobacter xylosoxidans is described as being resistant to antiseptics and disinfectants. We studied in vitro the ability of five strains to survive and grow in such solutions, with and without starvation. METHODS: Bacterial suspensions in rich media and in distilled water were inoculated into eight antiseptics or disinfectants under conditions of use. RESULTS: All strains from cultures in distilled water survived in aqueous chlorhexidine and only environmental strains survived in a quaternary ammonium-based disinfectant. Survival did not exceed 30 min and no growth was observed. CONCLUSIONS: This study highlights a relationship between starvation and survival in antiseptics and disinfectants.