RESUMO
Adipocytokines are substances produced by adipocytes responsible for the regulation of several physiological processes. Among those substances, leptin and adiponectin have been intensively investigated because of their possible effects on metabolism, reproduction, hematopoiesis and cardiovascular system. The current study aimed to assess the effects of adipocytokines, leptin and adiponectin on cardiac and respiratory functions, as well as on the hematological parameters of healthy female dogs of different breeds. Parameters of rectal temperature, diastolic blood pressure, systolic blood pressure, mean arterial pressure, pulse, heart rate and respiratory rate were evaluated in fifty two healthy female dogs. Blood samples were collected for complete blood count and plasma leptin and adiponectin measurement via ELISA technique. There was no difference in circulating levels of adipocytokines among the different breeds (p ≤ 0,05). Negative correlation between leptin and mean corpuscular hemoglobin concentration was found, while it was positive regarding systolic blood pressure, heart rate and breathing. Adiponectin was positively correlated with red blood cells and platelets and negatively with heart rate, respiratory rate, diastolic blood pressure and pulse. Adipocytokines, leptin and adiponectin, exert regulatory effects on cardiorespiratory function and hematopoiesis in healthy female dogs.(AU)
Assuntos
Animais , Feminino , Cães , Leptina/análise , Adiponectina/análise , Doenças Cardiovasculares/veterinária , Doenças Respiratórias/veterinária , Testes Hematológicos/veterinária , Ensaio de Imunoadsorção Enzimática/veterináriaRESUMO
Background: Due to the numerous complications that enteropexy may cause in domestic animals and humans, this study aimed at investigating the microscopic level, the damage generated by this technique in mice, in order to investigate the morphometric changes caused by enteropexy technique in rats. Materials, Methods & Results: Eighteen Wistar rats were submitted to surgical technique of enteropexy. To evaluate intestinal disorders they were euthanized at 30 (Group I), 60 (group II) and 90 (group III) days after the procedure. The animals were perfused with 2.5% glutaraldehyde and 4% paraformaldehyde for the removal of intestinal fragments for analysis in conventional microscopy and scanning electron microscopy. In classical histopathology, group I presented mild lesions with necrosis of the epithelium and infiltration of mononuclear cells. After 60 days, extensive ischemic necrosis area was observed, characterized by the denudation of epithelium cells and cellular debris in the lumen surface and loss of intestinal crypts. After 90 days (group III) lesions were evident and were characterized by strong coagulative necrosis of epithelial cells and intestinal crypts. Under electron microscopy, group I showed early fusion of the adjacent intestinal walls. After 60 days there was adherence of luminal stenosis and intestinal walls. In group III loss of epithelium and substitution [...](AU)
Assuntos
Animais , Camundongos , Mucosa Intestinal/cirurgia , Intussuscepção/veterinária , Obstrução Intestinal/veterinária , Complicações Pós-Operatórias/veterinária , Microscopia Eletrônica/veterináriaRESUMO
Background: Due to the numerous complications that enteropexy may cause in domestic animals and humans, this study aimed at investigating the microscopic level, the damage generated by this technique in mice, in order to investigate the morphometric changes caused by enteropexy technique in rats. Materials, Methods & Results: Eighteen Wistar rats were submitted to surgical technique of enteropexy. To evaluate intestinal disorders they were euthanized at 30 (Group I), 60 (group II) and 90 (group III) days after the procedure. The animals were perfused with 2.5% glutaraldehyde and 4% paraformaldehyde for the removal of intestinal fragments for analysis in conventional microscopy and scanning electron microscopy. In classical histopathology, group I presented mild lesions with necrosis of the epithelium and infiltration of mononuclear cells. After 60 days, extensive ischemic necrosis area was observed, characterized by the denudation of epithelium cells and cellular debris in the lumen surface and loss of intestinal crypts. After 90 days (group III) lesions were evident and were characterized by strong coagulative necrosis of epithelial cells and intestinal crypts. Under electron microscopy, group I showed early fusion of the adjacent intestinal walls. After 60 days there was adherence of luminal stenosis and intestinal walls. In group III loss of epithelium and substitution [...]