RESUMO

Six Plasmodium falciparum protein fractions, isolated under reducing conditions, were used to immunize mice, rabbits and the squirrel monkey Saimiri sciureus. Five or seven subcutaneous injections of each antigenic preparation, in conjunction with Freund's complete or incomplete adjuvants, were administered. This led to the development of specific antibodies detected by IFAT, ELISA or immunoblotting which inhibited merozoite reinvasion in in vitro P. falciparum cultures. This activity seems to be associated with rhoptry proteins contained in fractions Pf F2 and Pf F4.

Assuntos

Anticorpos Antiprotozoários/biossíntese , Antígenos de Protozoários/imunologia , Vacinas Antimaláricas/imunologia , Plasmodium falciparum/imunologia , Proteínas de Protozoários/imunologia , Animais , Anticorpos Antiprotozoários/imunologia , Feminino , Soros Imunes , Imunização Secundária , Imunoglobulina G/imunologia , Imunoglobulina M/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Plasmodium falciparum/crescimento & desenvolvimento , Coelhos , Saimiri , Vacinação