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1.
Tree Physiol ; 44(8)2024 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-38952005

RESUMO

Forest ecosystems face increasing drought exposure due to climate change, necessitating accurate measurements of vegetation water content to assess drought stress and tree mortality risks. Although Frequency Domain Reflectometry offers a viable method for monitoring stem water content by measuring dielectric permittivity, challenges arise from uncertainties in sensor calibration linked to wood properties and species variability, impeding its wider usage. We sampled tropical forest trees and palms in eastern Amazônia to evaluate how sensor output differences are controlled by wood density, temperature and taxonomic identity. Three individuals per species were felled and cut into segments within a diverse dataset comprising five dicotyledonous tree and three monocotyledonous palm species on a wide range of wood densities. Water content was estimated gravimetrically for each segment using a temporally explicit wet-up/dry-down approach and the relationship with the dielectric permittivity was examined. Woody tissue density had no significant impact on the calibration, but species identity and temperature significantly affected sensor readings. The temperature artefact was quantitatively important at large temperature differences, which may have led to significant bias of daily and seasonal water content dynamics in previous studies. We established the first tropical tree and palm calibration equation which performed well for estimating water content. Notably, we demonstrated that the sensitivity remained consistent across species, enabling the creation of a simplified one-slope calibration for accurate, species-independent measurements of relative water content. Our one-slope calibration serves as a general, species-independent standard calibration for assessing relative water content in woody tissue, offering a valuable tool for quantifying drought responses and stress in trees and forest ecosystems.


Assuntos
Florestas , Árvores , Clima Tropical , Água , Madeira , Madeira/química , Água/metabolismo , Árvores/fisiologia , Ecossistema , Secas , Arecaceae/fisiologia , Arecaceae/metabolismo , Brasil
2.
Braz J Microbiol ; 55(3): 2767-2782, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39012425

RESUMO

Norovirus is an important etiologic agent of acute gastroenteritis and has become even more relevant in Brazil after the implementation of the monovalent rotavirus vaccine in 2006 through the public health system, now representing a significant portion of the etiology of acute diarrheal diseases. Although diagnosing acute gastroenteritis caused by norovirus is a relatively simple process, and the infection tends to be self-limited, the virus can be considerably harmful to vulnerable populations, such as children, the elderly, and immunocompromised individuals. The spread of norovirus is also particularly favorable among such groups due to its mode of transmission, favored by cluttered environments such as in hospitals and densely populated regions. Additionally, norovirus' ability to spread through water and food creates the need for measures to ensure adequate sanitation and the development of effective measures to prevent outbreaks and severe manifestations of the disease. This review aims to address the main reports of human norovirus detected in Brazil over the years, focusing on clinical-hospital, food-related, and urban conglomerate contexts, including the circulating strains.


Assuntos
Infecções por Caliciviridae , Gastroenterite , Norovirus , Norovirus/genética , Norovirus/isolamento & purificação , Norovirus/classificação , Brasil/epidemiologia , Humanos , Infecções por Caliciviridae/epidemiologia , Infecções por Caliciviridae/virologia , Gastroenterite/virologia , Gastroenterite/epidemiologia , Surtos de Doenças
3.
J Med Virol ; 96(7): e29750, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38953413

RESUMO

The Phylum Cressdnaviricota consists of a large number of circular Rep-encoding single-stranded (CRESS)-DNA viruses. Recently, metagenomic analyzes revealed their ubiquitous distribution in a diverse range of eukaryotes. Data relating to CRESS-DNA viruses in humans remains scarce. Our study investigated the presence and genetic diversity of CRESS-DNA viruses in human vaginal secretions. Vaginal swabs were collected from 28 women between 29 and 43 years old attending a fertility clinic in New York City. An exploratory metagenomic analysis was performed and detection of CRESS-DNA viruses was confirmed through analysis of near full-length sequences of the viral isolates. A phylogenetic tree was based on the REP open reading frame sequences of the CRESS-DNA virus genome. Eleven nearly complete CRESS-DNA viral genomes were identified in 16 (57.1%) women. There were no associations between the presence of these viruses and any demographic or clinical parameters. Phylogenetic analysis indicated that one of the sequences belonged to the genus Gemycircularvirus within the Genomoviridae family, while ten sequences represented previously unclassified species of CRESS-DNA viruses. Novel species of CRESS-DNA viruses are present in the vaginal tract of adult women. Although they be transient commensal agents, the potential clinical implications for their presence at this site cannot be dismissed.


Assuntos
Vírus de DNA , Genoma Viral , Metagenômica , Filogenia , Vagina , Humanos , Feminino , Adulto , Vagina/virologia , Genoma Viral/genética , Vírus de DNA/genética , Vírus de DNA/classificação , Vírus de DNA/isolamento & purificação , DNA Viral/genética , Cidade de Nova Iorque , Análise de Sequência de DNA , Variação Genética
4.
Rev. Bras. Odontol. Leg. RBOL ; 11(1): 28-36, 20240601.
Artigo em Português | LILACS-Express | LILACS | ID: biblio-1556120

RESUMO

Introdução: Os dentes são órgãos de valor inestimável para a identificação humana, uma vez que são as estruturas mais estáveis, duras e resistentes do corpo. Com a existência progressiva para o uso de câmeras digitais para captar fotografias sociais, como as "selfie", em que o foco principal está direcionado no rosto do indivíduo, estas fotografias são capazes de prover material com capacidade para resolver casos de identificação humana. Objetivo: Demonstrar a importância das fotografias do sorriso no processo de identificação humana e comparar e verificar o desempenho de graduandos em Odontologia (ingressantes e concluintes) na identificação humana por meio da análise do sorriso. Material e Métodos: Este estudo utilizou a metodologia e banco de imagens do trabalho de Silva et al. (2012), e o público alvo que avaliou as imagens, modificada. Cada aluno participante da pesquisa analisou um grupo de 4 imagens projetadas, sendo 2 de sorriso (A e B) e 2 intrabucais (C e D). Dentre as 10 fornecidas para cada grupo de imagem, somente uma é compatível com cada fotografia projetada. Resultado: Após análise dos dados, foi possível verificar que 84,6% dos alunos ingressantes (número de 26 alunos) acertaram os testes A e B, e 95,6% dos concluintes (número de 23) acertaram os testes A e B. No teste C podemos observar que 61,5% dos alunos ingressantes acertaram o teste, e 69,6% dos concluintes obtiveram acertos. E, por fim, o teste D mostrou que os ingressantes obtiveram um total de 80,8% de acertos, enquanto todos concluintes (100%) acertaram o teste D. Os parâmetros mais citados foram a coloração dos dentes, a inclinação dental, a morfologia das incisais e o alinhamento incisal. Conclusão: Nesta pesquisa, tanto os alunos ingressantes como os concluintes demostraram capacidade para analisar as fotografias do sorriso na identificação humana. Os alunos ingressantes obtiveram um número maior de erros pelo fato de ainda não terem conhecimento teórico e técnico mais aprofundado em anatomia, tornando isso uma diferença entre ingressantes e concluintes


Introduction: Teeth are invaluable organs for human identification, as they are the most stable, hard and resistant structures in the body. With the progressive use of digital cameras to capture social photographs, such as the "selfie", in which the main focus is on the individual's face, these photographs are able to provide material capable of solving human identification cases. Objective: To demonstrate the importance of smile photographs in the process of human identification and to compare and verify the performance of undergraduate dental students (beginners and graduates) in human identification using smile analysis. Material and Methods: This study used the methodology and image bank of the work by SILVA R.F. et al. (2012), and the target audience that evaluated the images, modified. Each student participating in the study analyzed a group of 4 projected images, 2 of which were smiles (A and B) and 2 intraoral (C and D). Of the 10 provided for each image group, only one was compatible with each projected photograph. Results: After analyzing the data, it was possible to see that 84.6% of the incoming students (26 students) got tests A and B right, and 95.6% of the outgoing students (23) got tests A and B right. In test C we can see that 61.5% of incoming students got the test right, and 69.6% of outgoing students got it right. And finally, test D showed that the entrants got a total of 80.8% right, while all the graduates (100%) got test D right. The most cited parameters were tooth color, tooth inclination, incisal morphology and incisal alignment. Conclusion: In this study, both new students and graduates demonstrated their ability to analyze smile photographs in human identification. The new students had a higher number of errors, due to the fact that they did not yet have in-depth theoretical and technical knowledge of anatomy, making this a difference between new students and graduates

5.
Rev. bras. ortop ; 59(3): 415-419, May-June 2024. tab
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1569758

RESUMO

Abstract Objective To analyze the epidemiological data of orthopedic injuries in beach tennis players. Methods We conducted a cross-sectional study, through a questionnaire, with 185 beach tennis players during tournaments and training sessions in two cities. The questionnaire comprised anthropometric data, as well as data on length of time practicing the sport and reported orthopedic injuries. Results We excluded 25 of the 185 interviewees. Of the 160 players studied, 51.9% were male and 48.1%, female. The average age was of 40.4 years, the average height was of 1.73 m, the average weight was of 75.6 kg, and the average body mass index (BMI) was of 25.2 kg/m2. Orthopedic injuries were reported by 48.8% of the participants, 30.0% in the lower limbs, 11.3% in the spine, and 25.0% in the upper limbs, with an incidence of 0.82 per one thousand hours of practice. Correlating the incidence of injuries with age, weight, height, and BMI, we only found relevance between the higher average age in patients with lower- and upper-limb injuries. Players who had no previous experience with other racket sports had fewer injuries. Other parameters, such as gender, use of the two-handed backhand, dominant side, participation in competitions, and practice of other sports did not show statistically significant differences. Conclusion Orthopedic injuries were found in almost half of the beach tennis players, primarily in the lower limbs. Age, experience with other racket sports, category, hours of training per week, and length of time playing the sport influenced the incidence of orthopedic injuries.


Resumo Objetivo Analisar os dados epidemiolügicos das lesões ortopédicas nos praticantes de beach tennis. Métodos Foi realizado um estudo transversal, por meio de questionário, em 185 praticantes de beach tennis, durante torneios e treinos, em duas cidades. O questionário consistiu na coleta de dados antropométricos, além de dados relativos ao tempo de prática do esporte e às lesões ortopédicas referidas. Resultados Excluímos 25 dos 185 entrevistados. Dos 160 praticantes estudados, 51,9% eram do sexo masculino, e 48,1%, do feminino. A média de idade foi de 40,4 anos, a média da estatura foi de 1,73 m, a média do peso, de 75,6 kg, e a média do índice de massa corporal (IMC), de 25,2 kg/m2. A presença de lesões ortopédicas foi relatada por 48,8% dos praticantes, sendo 30,0% nos membros inferiores (MMII), 11,3% na coluna, e 25,0% nos membros superiores (MMSS), com incidência de 0,82 a cada mil horas de prática. Correlacionando a incidência de lesões com idade, peso, altura e IMC, encontramos relevância apenas entre a média de idade maior nos pacientes com lesão nos MMII e MMSS. Os jogadores que não tinham experiência prévia com outros esportes com raquete tiveram menos lesões. Demais parâmetros, como sexo, uso do backhand com duas mãos, lado dominante, participação em competições e prática de outros esportes não obtiveram diferenças estatisticamente significantes. Conclusão Lesões ortopédicas foram encontradas em quase a metade dos praticantes de beach tennis, preferencialmente nos MMII. Idade, experiência com outros esportes de raquete, categoria, horas de treino por semana e tempo de prática do esporte influenciaram na incidência de lesões ortopédicas.

7.
Avian Pathol ; : 1-13, 2024 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-38916258

RESUMO

RESEARCH HIGHLIGHTS: IDS presented pathognomonic dilatation of the jejunum up to Meckel's diverticulum.IDS caused weight loss, decreased egg production, and increased culling and mortality.Chicken parvovirus (ChPV) was consistently detected through PCR assays.Chicken megrivirus (ChMV) was consistently detected through viral metagenomics.

8.
Rev Bras Ortop (Sao Paulo) ; 59(3): e415-e419, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38911894

RESUMO

Objective To analyze the epidemiological data of orthopedic injuries in beach tennis players. Methods We conducted a cross-sectional study, through a questionnaire, with 185 beach tennis players during tournaments and training sessions in two cities. The questionnaire comprised anthropometric data, as well as data on length of time practicing the sport and reported orthopedic injuries. Results We excluded 25 of the 185 interviewees. Of the 160 players studied, 51.9% were male and 48.1%, female. The average age was of 40.4 years, the average height was of 1.73 m, the average weight was of 75.6 kg, and the average body mass index (BMI) was of 25.2 kg/m 2 . Orthopedic injuries were reported by 48.8% of the participants, 30.0% in the lower limbs, 11.3% in the spine, and 25.0% in the upper limbs, with an incidence of 0.82 per one thousand hours of practice. Correlating the incidence of injuries with age, weight, height, and BMI, we only found relevance between the higher average age in patients with lower- and upper-limb injuries. Players who had no previous experience with other racket sports had fewer injuries. Other parameters, such as gender, use of the two-handed backhand , dominant side, participation in competitions, and practice of other sports did not show statistically significant differences. Conclusion Orthopedic injuries were found in almost half of the beach tennis players, primarily in the lower limbs. Age, experience with other racket sports, category, hours of training per week, and length of time playing the sport influenced the incidence of orthopedic injuries.

9.
Microsurgery ; 44(5): e31201, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38886919

RESUMO

Congenital pseudarthrosis of the forearm poses a considerable challenge because of its rarity. The objective of this report is to introduce a novel surgical technique for its treatment. Here, we document a case of congenital pseudarthrosis of the radius in a 3-year-old boy diagnosed with type-1 neurofibromatosis. The surgical treatment involved the excision of approximately 9 cm of native radial periosteum and a bifocal radius osteotomy, which was supplemented with a vascularized tibial periosteal transplant to facilitate bone healing. Anastomosis between the anterior tibial vessels and radial vessels was performed. No immediate or late postoperative complications were observed. After 3 weeks, a robust callus formation was observed, and during a follow-up examination 3 years and 4 months later, a wide range of active forearm rotation was noted. This report suggests that vascularized periosteal flaps show promise as a viable treatment option for congenital pseudarthrosis of the forearm. They offer an alternative to vascularized fibular grafts or single-bone forearm constructs.


Assuntos
Periósteo , Pseudoartrose , Tíbia , Humanos , Pseudoartrose/congênito , Pseudoartrose/cirurgia , Masculino , Pré-Escolar , Periósteo/transplante , Tíbia/cirurgia , Neurofibromatose 1/complicações , Neurofibromatose 1/cirurgia , Procedimentos de Cirurgia Plástica/métodos , Retalhos Cirúrgicos/irrigação sanguínea , Retalhos Cirúrgicos/transplante , Osteotomia/métodos , Rádio (Anatomia)/transplante , Rádio (Anatomia)/cirurgia , Rádio (Anatomia)/anormalidades , Transplante Ósseo/métodos
10.
Foods ; 13(11)2024 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-38890933

RESUMO

The composition of pumpkin seeds includes bioactive compounds, proteins, polyunsaturated fatty acids, and dietary fibers. Thus, the objective of this research was to develop and evaluate the nutritional and quality standard of beef burgers supplemented with pumpkin seeds (Cucurbita moschata) added in different proportions. To process the pumpkin seed flour (PSF), the seeds were sanitized, dried in an oven, crushed, and sieved. Through such means, three formulations of beef burgers were prepared, named S (without the addition of PSF), F5 (with the addition of 5% (w/w) of PSF), and F10 (with the addition of 10% (w/w) of PSF). The respective results for burgers P, F5, and F10 were as follows (w/w): proteins 17.61%, 18.04%, 19.86%; lipids 12.19%, 12.42%, 14.55%; ash 1.77%, 1.86%, 1.94%; fibers 0%, 0.88%, 1.76%; phenolic compounds 39.55, 82.93, 90.30 (mg/g); and total antioxidant capacity 11.09%, 18.48%, 24.45%. Regarding the sensory analysis attributes, tasters gave sample F10 scores lower than 7. However, the standard and F5 samples showed results higher than 7 for all parameters. For the determination of shelf life, an expiration date of 30 days was established. It was observed that adding PSF to industrialized products adds nutritional value with the inclusion of polyunsaturated fats, phenolic compounds, and dietary fibers.

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