RESUMO
Alzheimer's disease (AD) was established as the neurodegenerative dementia, which leads to progressive and irreversible cognitive decline, memory loss, attention and judgment. It is characterized by molecular injuries resulting from the accumulation of charged neuritic plaques of β-amyloid and neurofibrillary tangles causing oxidative damage and inflammatory. Thus, the aim of this study was to evaluate the learning and memory of rats seven days after infusion of β-amiloide1-42. The β-amiloide1-42 was prepared in saline protein (1ug / uL) and incubated at 37 ° C for three days to form the aggregate. The animals were anesthetized with the combination of ketamine (100 mg / kg) and xylazine (10 mg / kg) intraperitoneally, and fixed in a stereotaxic route. The aggregate was infused directly in the hippocampus (5UL bilaterally) in the coordinates, AP: -3.5 mm; LL: ± 2.0 mm; DV: -3.5. The learning and memory was assessed using the Morris water maze. The apparatus consists of a circular water tank (120 cm diameter and 60 cm high) with an escape platform (12.5 cm in diameter and 38 cm high) positioned invisible to the mice 2 cm below the water level. The tank was divided by imaginary lines (N, S, L, and O) in four quadrants (1, 2, 3 and 4) and identified by the same geometrical symbols, which remained the same throughout the study. The animals were placed facing the geometric shapes and the exhaust time measured during four consecutive days (training). In the fifth and final day it was taken to the escape platform and computed the total residence time in the target quadrant (test). The maximum time for each step was 60s. This study was approved by Ethics Committee for Animal Use (CEUA) of the State University of Ceará (UECE) by the number 2542310/2015. To analyze the learning we used the ANOVA two way with Bonferroni post-test. To verify differences in memory between the groups we used the Student t test. P <0.05 significance level was adopted[...](AU)
Assuntos
Animais , Ratos , Doença de Alzheimer , Peptídeos beta-Amiloides , Memória , Degeneração Neural , Doenças do Sistema NervosoRESUMO
Alzheimer's disease (AD) was established as the neurodegenerative dementia, which leads to progressive and irreversible cognitive decline, memory loss, attention and judgment. It is characterized by molecular injuries resulting from the accumulation of charged neuritic plaques of β-amyloid and neurofibrillary tangles causing oxidative damage and inflammatory. Thus, the aim of this study was to evaluate the learning and memory of rats seven days after infusion of β-amiloide1-42. The β-amiloide1-42 was prepared in saline protein (1ug / uL) and incubated at 37 ° C for three days to form the aggregate. The animals were anesthetized with the combination of ketamine (100 mg / kg) and xylazine (10 mg / kg) intraperitoneally, and fixed in a stereotaxic route. The aggregate was infused directly in the hippocampus (5UL bilaterally) in the coordinates, AP: -3.5 mm; LL: ± 2.0 mm; DV: -3.5. The learning and memory was assessed using the Morris water maze. The apparatus consists of a circular water tank (120 cm diameter and 60 cm high) with an escape platform (12.5 cm in diameter and 38 cm high) positioned invisible to the mice 2 cm below the water level. The tank was divided by imaginary lines (N, S, L, and O) in four quadrants (1, 2, 3 and 4) and identified by the same geometrical symbols, which remained the same throughout the study. The animals were placed facing the geometric shapes and the exhaust time measured during four consecutive days (training). In the fifth and final day it was taken to the escape platform and computed the total residence time in the target quadrant (test). The maximum time for each step was 60s. This study was approved by Ethics Committee for Animal Use (CEUA) of the State University of Ceará (UECE) by the number 2542310/2015. To analyze the learning we used the ANOVA two way with Bonferroni post-test. To verify differences in memory between the groups we used the Student t test. P <0.05 significance level was adopted[...]