RESUMO
Taurine concentrations were determined in gliomas from 16 patients and in meningiomas from 15 patients. After imaging analysis and clinical evaluation to consider the level of functional deterioration by the scale of Karnosky, tissue was obtained by surgery. Tumoral, peritumoral and extratumoral samples were taken and analyzed by HPLC with fluorescence detector. The concentration of taurine (nmol/mg protein) was higher in tumoral and peritumoral tissues than in the extratumoral samples for gliomas. In the case of meningiomas, the taurine concentration was higher in tumoral than in peritumoral and extratumoral samples. These modifications might be due to specific functions of this amino acid, being either protective or involved in the proliferation of cells. The differential distribution in the two types of tumors could be related to the malignancy of them, which is higher in gliomas than in meningiomas.
Assuntos
Neoplasias Encefálicas/química , Glioma/química , Neoplasias Meníngeas/química , Meningioma/química , Taurina/análise , Adolescente , Adulto , Idoso , Neoplasias Encefálicas/patologia , Glioma/patologia , Humanos , Processamento de Imagem Assistida por Computador , Masculino , Neoplasias Meníngeas/patologia , Meningioma/patologia , Pessoa de Meia-IdadeRESUMO
The medium from cultured optic tectum of the goldfish intact or at various days after optic nerve crush, or the co-culture of this optic tectum with post-crush goldfish retinal explants plating in the absence of fetal calf serum, but in the presence of glucose, modulate its outgrowth. The a dition of taurine did not further stimulate outgrowth but rather inhibited it in the presence of optic tectum. These processes were related to calcium fluxes and taurine transport into the cells. Taurine increased the length of neurites from 5-day-old rat retinal explants in the presence of fetal calf serum. The goldfish optic tectum, either medium or in co-culture with the retina, stimulated retinal outgrowth. The study of optic nerve regeneration in the presence of defined media contributes to understanding tissue-target and interspecies interaction.
Assuntos
Meios de Cultura/química , Carpa Dourada/anatomia & histologia , Retina/metabolismo , Colículos Superiores/metabolismo , Taurina/metabolismo , Técnicas de Cultura de Tecidos , Animais , Quelantes/metabolismo , Técnicas de Cocultura , Ácido Egtázico/metabolismo , Carpa Dourada/metabolismo , Nervo Óptico/patologia , Nervo Óptico/cirurgia , RatosRESUMO
Post-crush goldfish retinal explants were cultured in the absence of fetal calf serum and in the presence of calcium, albumin, glucose or taurine. There was an increase in length and density of the neurites in a dose-dependent manner when fetal calf serum was added to the medium. The addition of calcium to the basic medium, Leibovitz, produced a small increase in neuritic outgrowth at low concentrations, but inhibited outgrowth at higher levels. Albumin did not significantly modify neuritic outgrowth. Glucose, at variable concentrations, produced a bell-shaped increase in length and density of the neurites. Taurine, in the absence of fetal calf serum, produced a small increase in neuritic outgrowth at 10 mM. In the presence of glucose, taurine stimulated outgrowth was less efficient than in the presence of fetal calf serum. In order to investigate the mechanisms of action of taurine as a trophic agent, the culture medium in the absence of fetal calf serum will be supplemented with glucose.