RESUMO

OBJECTIVES: To evaluate the DNA damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated buccal mucosa cells from children undergoing orthodontic radiographs. MATERIALS AND METHODS: A total of 25 healthy children undergoing orthodontic therapy partook in a complete set of orthodontic radiographs (lateral cephalographic, posteroanterior cephalographic, panoramic, full periapical exam, and bitewing). The micronucleus test in the buccal exfoliated cells was applied. The paired-samples t-test and the Wilcoxon test were used to compare the frequencies of alterations before and after X-ray exposure. RESULTS: We found no statistically significant differences (P > .05) between micronucleated buccal mucosa cells before and after exposure to radiation. However, radiation did cause other nuclear alterations closely related to cytotoxicity (P  =  .007). CONCLUSION: According to the micronucleus test, the complete set of radiographs requested in the orthodontic planning may not be a factor that induces chromosomal damage, but it is able to promote cytotoxicity.

Assuntos

Núcleo Celular/efeitos da radiação , Dano ao DNA , Micronúcleos com Defeito Cromossômico/efeitos da radiação , Testes para Micronúcleos/métodos , Mucosa Bucal/citologia , Radiografia Dentária/efeitos adversos , Biomarcadores/análise , Morte Celular/genética , Morte Celular/fisiologia , Morte Celular/efeitos da radiação , Criança , Análise Citogenética , Feminino , Humanos , Masculino , Mucosa Bucal/diagnóstico por imagem , Mucosa Bucal/efeitos da radiação , Ortodontia/métodos