RESUMO
Broiler chickens aged 41-day-old from a flock of a broiler complex, presenting depression, reduced food intake, facial edema, dyspnea, gasping, sneezing, and 5% mortality were studied. At necropsy, opaque thoracic and abdominal air sacs and mucous tracheal content were observed. Histopathology of tracheas showed multifocal hyperplasia of mucosa with a large number of small, round and ovoid basophilic organisms on their surface, which were identified as Cryptosporidium spp. In addition, there was an inflammatory response due to infiltration of mononuclear cells and heterophils in the submucosa. Small pin-point colonies without hemolytic activity were isolated from tracheal samples and identified as Ornithobacterium rhinotracheale by conventional and real time polymerase chain reaction (PCR). Results of tracheal histopathology, bacteriology, and PCR identification provided the diagnosis of tracheal cryptosporidiosis associated with non-hemolytic O. rhinotracheale secondary infection. This report describes the unusual dual infection with Cryptosporidium spp. and non-hemolytic O. rhinotracheale causing tracheitis in broiler chickens.
RESUMO
A 2-year-old captive, male Eclectus parrot (Eclectus roratus), with a previous history of neurologic signs episodes, was found dead in its cage without premonitory signs in a private aviary. At necropsy, yellow and caseous nodules were observed in the lungs. Microscopically, the nodules were composed of granulomas that had a necrotic center with intralesional hyphae typical of Aspergillus spp. and a peripheral inflammatory cell response composed of macrophages and multinucleated giant cells. The mycologic culture allowed the isolation and identification of A. flavus from lung samples. The gross and microscopic lesions, in combination with the mycologic identification provided the diagnosis of pulmonary aspergillosis due to A. flavus infection. To the authors´ knowledge, this is the first report of mycotic pneumonia caused by A. flavus infection in Psittaciformes.
RESUMO
Broiler chickens aged 40 and 46-days-old from two neighboring flocks belonging to a commercial broiler complex, presenting facial edema, severe respiratory signs and 10% mortality were analyzed. Pneumonic lesions and opaque thoracic and abdominal air sacs with foamy exudate were seen at postmortem examination. Histopathology of lungs showed fibrinoheterophilic pneumonia in both cases. Small pin point colonies with extensive -hemolytic activity were isolated from tracheal and lung tissue samples and identified as Ornithobacterium rhinotracheale by polymerase chain reaction (PCR). Results of lung histopathology, bacteriological isolation and PCR identification confirmed the diagnosis of pneumonia caused by -hemolytic O. rhinotracheale infection in both broiler chicken flocks. This paper appears to be the first report of -hemolytic O. rhinotracheale field isolates obtained from broiler chickens associated with severe respiratory signs and pneumonia.