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Plant Biol (Stuttg) ; 7(1): 104-7, 2005 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-15666217

RESUMO

We identified a transgenic line exhibiting albinism during our work to introduce genes through genetic engineering in dry bean (Phaseolus vulgaris). The transgenic mother plant (R0) presented a normal phenotype and generated albino and normal green plants in the first generation (R1). The segregation ratio of the albino character in the R1 and R2 generations fitted the expected ratio for a character controlled by a single recessive gene linked to a foreign gus gene, suggesting that albinism could be a consequence of insertional mutation caused by introduction of the exogenous gene. Analysis by electron microscope revealed that the albino cells possessed no chloroplasts and a greater number of mitochondria when compared to normal green plants. This transgenic bean line may be used in understanding the genetic control of chloroplast genesis, for acquiring additional knowledge of genomic structure or in physiological studies. This is the first described transgene-associated mutant bean plant.


Assuntos
Cloroplastos/genética , Genes Recessivos/fisiologia , Phaseolus/genética , Plantas Geneticamente Modificadas/genética , Transgenes/fisiologia , Cloroplastos/fisiologia , Cor , Expressão Gênica , Mitocôndrias/genética , Mitocôndrias/fisiologia , Mutação , Phaseolus/fisiologia , Phaseolus/ultraestrutura , Fenótipo , Plantas Geneticamente Modificadas/fisiologia , Plantas Geneticamente Modificadas/ultraestrutura
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