RESUMO
This study aims to evaluate the infection's clinical parameters and genetic diversity of msp1α of the AmRio1 strain in acute and chronic infections in cattle and ticks. A calf experimentally infected with the A. marginale AmRio1 strain was monitored during acute infection, and the presence of the msp1α gene was verified in the semi-nested polymerase chain reaction (PCR) during the acute and persistent phases of infection. From day seven after inoculation of the pathogen, the calf showed an increase in body temperature, decrease in hematocrit and increase in the percentage of cells infected by the agent, as well as clinical signs. Blood samples from the experimentally infected calf were positive during the acute infection and the persistent PCR infection for the msp1α gene. During the acute phase, infestation with Rhipicephalus microplus was performed. To evaluate the chronic phase, a blood sample was collected at 90â¯days post-infection. There was no variation of the MSP1a protein in this study. The AmRio1 strain was pathogenic as it caused severe changes in the clinical parameters of the monitored cattle. The positivity of this strain in organs and saliva of the analyzed ticks indicates a probable biological transmission.
Assuntos
Anaplasma marginale/genética , Anaplasmose/microbiologia , Vetores Aracnídeos/microbiologia , Proteínas da Membrana Bacteriana Externa/genética , Doenças dos Bovinos/microbiologia , Rhipicephalus/microbiologia , Sequência de Aminoácidos , Anaplasma marginale/classificação , Anaplasmose/transmissão , Animais , Proteínas da Membrana Bacteriana Externa/química , Bovinos , Doenças dos Bovinos/sangue , Doenças dos Bovinos/transmissão , Reação em Cadeia da Polimerase , Saliva/microbiologiaRESUMO
Although the largest buffalo herd in the occident is in the north region of Brazil, few studies have been conducted to assess the prevalence of selected parasitic diseases in buffalo herd. The present study was therefore conducted to investigate the epidemiological of Toxoplasma gondii, Neospora caninum, Anaplasma marginale, Babesia bigemina, and Babesia bovis in water buffaloes in the north region of Brazil. A total of 4796 buffalo blood samples were randomly collected from five provinces and simultaneously analyzed by the IFAT and ELISA. The serological prevalence of T. gondii and N. caninum was 41.3% and 55.5% in ELISA and 35.7% and 48.8% in IFAT, respectively. The overall prevalence of A. marginale, B. bovis, and B. bigemina was 63%, 25%, and 21% by ELISA and 50.0%, 22.5%, and 18.8% by IFAT, respectively. This study shows valuable information regarding the serological survey of selected bovine pathogens in water buffaloes in the north region of Brazil which will likely be very beneficial for the management and control programs of this disease.