RESUMO
The management of skin burns is still challenging. Among the therapeutic methods used, there are topical treatments with pharmacological and herbal agents, low-intensity therapeutic ultrasound, use of biomaterials, reconstructive techniques and photobiomodulation therapy. The aim of this study was to evaluate the effects of photobiomodulation with blue Light Emitting Diode (LED) on burn healing. Fifty Wistar rats were divided into control (CTRL) (n = 25) and blue LED (LED) (n = 25), with subgroups (n = 5) for each time of euthanasia (7, 14, 21, 28 and 32 days). Treated animals were daily irradiated (470 nm, 1W, 0.44 W/cm2, 50 J/cm2). Clinical evaluations were performed and the Wound Retraction Index (WRI) was determined. Histological sections were submitted to hematoxylin-eosin, toluidine blue and the immunohistochemical technique, with anti-α-SMA and anti-TGF-ß1 antibodies. All data were directly collected by previously calibrated evaluators in a blind manner. The values were included in a statistical program. For all statistical tests used, 5% significance level (p < 0.05) was considered. No statistically significant differences in WRI between groups were observed (p > 0.05). Re-epithelialization was higher using LED at 7 and 14 days (p < 0.05) and greater amount of inflammatory cells was observed at 7 days (p = 0.01). With LED at 21 and 32 days, greater number of mast cells were observed (p < 0.05), as well as smaller number of myofibroblasts at 14, 21, 28 and 32 days (p < 0.05) and lower percentage of TGF-ß1 positive cells in the conjunctiva at 7, 14 and 21 days (p < 0.05). Negative correlations were observed in LED between the percentage of TGF-ß1 in the epithelium and the mean number of inflammatory cells and number of myofibroblasts (p < 0.05). The results suggest that, depending on the period, blue LED can modulate the healing processes of third-degree skin burns, such as re-epithelialization, inflammatory response, mast cell concentration, myofibroblast differentiation and TGF-ß1 immunoexpression. Despite these effects, this therapy does not seem to have significant influence on the retraction of these wounds. Future studies, using different protocols, should be carried out to expand the knowledge about the photobiomodulatory mechanisms of this type of light in the healing process.
Assuntos
Queimaduras , Terapia com Luz de Baixa Intensidade , Ratos , Animais , Fator de Crescimento Transformador beta1/metabolismo , Ratos Wistar , Cicatrização , Pele/patologia , Queimaduras/radioterapiaRESUMO
Actinic cheilitis (AC) and lower lip squamous cell carcinoma (LLSCC) exhibit epithelial alterations mainly associated with chronic ultraviolet ray exposure. Currently, it is impossible to predict which AC cases will progress to LLSCC; thus, biomolecular markers have been studied. The aim of this study was to evaluate the immunoexpression of IMP-3 and KI-67 in AC and LLSCC. Forty AC and 40 LLSCC cases were submitted to peroxidase method and quantitatively analyzed, using the following scores: 0 (0% positive cells), + 1(≤ 30%), + 2 (> 30% to ≤ 60%), and + 3 (> 60%). Results were submitted to non-parametric Mann-Whitney (U) test. A p value < 0.05 was considered to indicate statistical significance. IMP-3 immunoexpression was observed in 26 AC cases, with predominance of the score 1 (35% of cases). This protein was also positive in 22 LLSCC cases, with predominance of the score 3 (37.5% of cases). Immunoexpression of KI-67 was observed in all studied cases, with predominance of the score 2 (70% of AC cases and 57.5% LLSCC cases). The association between IMP-3 and Ki-67 immunoexpression, AC dysplastic severity and LLSCC tumoral grade revealed no significant differences. The present results demonstrate that IMP-3 and Ki-67 immunoexpression are frequent in AC and in LLSCC. Moreover, these proteins could be involved in lower lip carcinogenesis process.
Assuntos
Carcinoma de Células Escamosas , Queilite , Neoplasias Labiais , Humanos , Carcinoma de Células Escamosas/diagnóstico , Queilite/diagnóstico , Antígeno Ki-67 , Lábio/patologia , Neoplasias Labiais/diagnósticoRESUMO
Introdução: O fibroma de células gigantes é uma neoplasia fibrosa benigna, considerada rara, com fatores etiológicos incertos e características clinico-patológicas peculiares. Objetivo: Descrever a exérese do fibroma de células gigantes, em mucosa jugal direita, utilizando laser cirúrgico. Relato de caso: Paciente do sexo feminino, 33 anos, parda, atendida na clínica de Estomatologia da Universidade Estadual da Paraíba, motivada por uma lesão neoplásica, de crescimento lento em região de mucosa jugal direita. Clinicamente, observou-se massa tumoral única, assintomática, com aproximadamente dois centímetros, de base séssil, normocorada, de consistência firme e superfície lisa. Após exame clínico, foi realizada uma biópsia excisional com fins diagnósticos, utilizando o laser cirúrgico. O diagnóstico, após o resultado do exame histopatológico, revelou um fibroma de células gigantes. A abordagem da biópsia excisional, além de ter fins de diagnóstico bucal, foi responsável pelo tratamento da lesão, visto que proporcionou a remoção completa da patologia. Optou-se por cicatrização por segunda intenção, e para acelerar esse processo, foi realizada aplicação local com laser de baixa potência de espectro de luz vermelha. No acompanhamento de sete dias, observou-se cicatrização adequada, com mínima alteração tecidual. Após oito meses, notou-se regeneração tecidual adequada sem recidiva da lesão. Conclusão: A remoção de um fibroma de células gigantes, utilizando laser de diodo de alta potência, se mostrou como uma abordagem terapêutica viável para o tratamento dessa patologia(AU)
Assuntos
Humanos , Feminino , Adulto , Células Gigantes/patologia , Diagnóstico Bucal/métodos , Fibroma/diagnóstico por imagem , Terapia a Laser/métodosRESUMO
INTRODUCTION: Cripto-1 is a member of the epidermal growth factor-Cripto-1/FRL-1/Cryptic family. Besides being critical for early embryonic development, Cripto-1 is also associated with the development and behavior of several cancers. OBJECTIVE: We analyzed the immunoexpression of Cripto-1 in normal salivary glands (NSGs), pleomorphic adenomas (PAs), and carcinoma ex pleomorphic adenomas (CaExPAs) of salivary glands. METHODS: A total of 12 NSGs, 16 PAs and 12 CaExPAs underwent immunohistochemical study by the polymeric biotin-free technique. Immunopositive cells were evaluated semiquantitatively (scores 0-3). For statistical analysis, Mann-Whitney and Kruskal-Wallis tests were performed and a significance level of p ≤ 0.05 was established. RESULTS: Most CaExPAs (n = 10) were strong positive (score 3) for Cripto-1, and only three cases of PAs and two specimens of NSGs exhibited some expression (score 1), being statistically significant these findings (p < 0.001). No difference between the expression of this protein in tumors of major and minor salivary glands was observed. Overexpression was found mainly in cases of CaExPAs with invasive growth (n = 8) when compared to those without capsular invasion (intracapsular pattern) (p = 0.036). Patients with or without lymph node metastasis showed no difference (p = 0.294). CONCLUSION: The results revealed a significantly higher expression of Cripto-1 in CaExPA compared to PA and NSG, suggesting this protein is possibly deregulated in PA malignant transformation. Furthermore, the increased expression of this protein is associated with a more aggressive behavior (invasive growth) in salivary gland tumors.
Assuntos
Adenocarcinoma/metabolismo , Adenoma Pleomorfo/metabolismo , Proteínas Ligadas por GPI/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Proteínas de Neoplasias/metabolismo , Neoplasias das Glândulas Salivares/metabolismo , Adenocarcinoma/patologia , Adenoma Pleomorfo/patologia , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Neoplasias das Glândulas Salivares/patologia , Glândulas Salivares Menores/metabolismo , Glândulas Salivares Menores/patologiaRESUMO
OBJECTIVE: to evaluate the association between XPD and XRCC3 polymorphisms and oral squamous cell carcinoma (OSCC). DESIGN: the sample consisted of 54 cases of OSCC and 40 cases of inflammatory fibrous hyperplasia (IFH). Genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. RESULTS: XPD-Lys/Gln was more common in IFH (n=28; 70%) than in OSCC (n=24; 44.4%) (OR: 0.3; p<0.05). XPD-Gln was more frequent in high-grade lesions (0.48) than in low-grade lesions (0.21) (OR: 3.4; p<0.05). The Gln/Gln genotype was associated with III and IV clinical stages (OR: 0.07; p<0.05). XRCC3-Met was more frequent in OSCC (0.49) than in IFH (0.35) (OR: 2.6; p<0.05). The Met/Met genotype was associated with the presence of metastases (OR: 8.1; p<0.05) and with III and IV clinical stages (OR: 0.07; p<0.05). CONCLUSIONS: in this sample, the frequency of XPD-Gln in IFH suggests that this variant may protect against OSCC. The presence of the XRCC3-Met allele seems to contribute to the development of OSCC, metastases and more advanced stages in these lesions.
Assuntos
Carcinoma de Células Escamosas/genética , Proteínas de Ligação a DNA/genética , Neoplasias de Cabeça e Pescoço/genética , Neoplasias Bucais/genética , Proteína Grupo D do Xeroderma Pigmentoso/genética , Brasil , Carcinoma de Células Escamosas/patologia , Reparo do DNA , DNA de Neoplasias/genética , DNA de Neoplasias/isolamento & purificação , Feminino , Frequência do Gene , Predisposição Genética para Doença , Genótipo , Neoplasias de Cabeça e Pescoço/patologia , Humanos , Masculino , Neoplasias Bucais/patologia , Metástase Neoplásica , Projetos Piloto , Reação em Cadeia da Polimerase/métodos , Polimorfismo Genético , Polimorfismo de Fragmento de Restrição , Carcinoma de Células Escamosas de Cabeça e PescoçoRESUMO
The aim of the present study was to compare the expression of α2ß1, α3ß1, and α5ß1 integrins between 28 pleomorphic adenomas (PAs) and 10 adenoid cystic carcinomas (ACCs), and investigate differences in the expression of these integrins according to histologic subtypes of ACCs. It was taken into consideration the presence or absence, distribution, and localization of integrin immunoexpression. There was immunoreactivity in the intercellular contacts of the strands, nests, and solid sheets of PAs, as well as in the luminal and nonluminal cells of the duct-like structures, with a predominant immunoexpression in the luminal cells. The immunoexpression in ACCs varied with histologic subtype of the tumor. It was verified for a tendency of absence and/or reduced expression of all integrins in the solid subtype of ACCs. In general, PAs revealed a more diffuse and remarkable immunoexpression of all studied integrins than ACCs. The reduced integrins expression in ACC may be related to a lesser degree of cell differentiation in this neoplasm. Moreover, the absence and/or reduced expression of the studied integrins in solid ACC suggest a possible role in pathogenesis and more aggressive biological behavior of this histologic subtype.
Assuntos
Adenoma Pleomorfo/genética , Biomarcadores Tumorais/genética , Carcinoma Adenoide Cístico/genética , Integrina alfa2beta1/genética , Integrina alfa3beta1/genética , Integrina alfa5beta1/genética , Neoplasias das Glândulas Salivares/genética , Adenoma Pleomorfo/diagnóstico , Adenoma Pleomorfo/patologia , Carcinoma Adenoide Cístico/diagnóstico , Carcinoma Adenoide Cístico/patologia , Expressão Gênica , Humanos , Imuno-Histoquímica , Neoplasias das Glândulas Salivares/diagnóstico , Neoplasias das Glândulas Salivares/patologiaRESUMO
It is estimated that more than 500 bacterial species inhabit the human oral environment. Among them, more than 400 species are known, and also a huge diversity of microorganisms that have been discovered because of the innovations in molecular biology techniques. Talking about staphylococcus spp., its presence in oral environment of health individuals and local or systemic wounded ones is at least controversial, what guides to the necessity of accurate studies in order to clarify this bacteria profile in this microenvironment ecology, as well as in oral and systemic disease etiology. This study aimed to gather the current knowledge concerning the staphylococcal presence in oral environment and its implications in oral mucositis development, in this case called "oral staphylococcal mucositis", a clinical condition that affects more commonly ancient patients, systemic weakened or immunologically wounded
Se estima que más de 500 especies bacterianas habitan el medio ambiente oral de seres humanos. Entre ellas, son conocidas más de 400 especies, además de una gran diversidad de microorganismos que han sido descubiertos gracias a innovaciones en las técnicas de biología molecular. Con relación a los staphylococcus spp., su presencia en el medio ambiente oral de individuos saludables, local o sistémicamente comprometidos es bastante controversia, lo que remite a la necesidad de estudios cuidadosos con intención de aclarar el papel de esas bacterias en la ecología de ese microambiente, así como en la etiología de enfermedades orales y sistémicas. Ese estudio propuso reunir los conocimientos actuales sobre la presencia de staphylococcus en el medio ambiente oral y sus implicaciones en el desarrollo de mucositis oral, denominada en ese caso de "mucositis estafilocócica oral", una condición clínica que afecta más comúnmente pacientes mayores, sistémicamente debilitados o inmunológicamente comprometidos