RESUMO
We examined the asymptomatic rates of SARS-CoV-2 infection during the Delta and Omicron waves in the city of São Paulo. Nasopharyngeal swabs were collected at strategic points of the city (open-air markets, bus terminals, airports) for SARS-CoV-2 RNA testing. Applying the questionnaire, the symptomatic individuals were excluded, and only asymptomatic cases were analyzed. During the Delta wave, a total of 4315 samples were collected, whereas 2372 samples were collected during the first Omicron wave. The incidence of the asymptomatic SARS-CoV-2 infection was 0.6% during the Delta wave and 0.8% during the Omicron wave. No statistical differences were found in the threshold amplification cycle. However, there was a statistical difference observed in the sublineage distribution between asymptomatic and symptomatic individuals. Our study determined the incidence of asymptomatic infection by monitoring individuals who remained symptom-free, thereby providing a reliable evaluation of asymptomatic SARS-CoV-2 carriage. Our findings reveal a relatively low proportion of asymptomatic cases, which could be attributed to our rigorous monitoring protocol for the presence of clinical symptoms. Investigating asymptomatic infection rates is crucial to develop and implement effective disease control strategies.
Assuntos
COVID-19 , Humanos , COVID-19/diagnóstico , COVID-19/epidemiologia , Brasil/epidemiologia , Infecções Assintomáticas/epidemiologia , RNA Viral/genética , SARS-CoV-2/genética , GenômicaRESUMO
In Peru, there is low dental service use among older adults, in addition to a social gradient for use. Furthermore, the COVID-19 pandemic triggered complex scenarios characterized by inadequate access to health services, with greater impact on vulnerable population groups such as the elderly. The aim of this study was to determine the impact of the COVID-19 pandemic on the time since last dental care visit among elderly Peruvians. It was a cross-sectional study conducted on a population of 5247 respondents in 2019, and 5066 respondents in 2020. The study considered the answers of people who responded to the question on the last time they had received dental care, extracting a total 4045 subjects for 2019 and 3943 for 2020. The dependent variable was time since last dental care measured in years, while the variables on health, geographic and sociodemographic characteristics were grouped within three dimensions. A descriptive bivariate and multivariateanalysiswasapplied by means of multiple linear regression to analyze the behavior of the variables. Time since the last dental care visit was 7.93 years (SD=8.03) in 2019 and 7.94 years (SD=7.28) in 2020. A hierarchical multiple linear regression analysis was performed, where no variable in the 2019 model was significant; while for 2020, health, geographic, and sociodemographic characteristics variables were significant. In the analysis where the 'year' variable becomes independent, only model 4, which considers all variables, was valid (p=0.018). The variables 'area of residence' and 'wealth index' were also significant. To conclude, the 2020 pandemic year for COVID-19 had no impact on the time since last dental care visit among elderly Peruvians, though factors such as area of residence and wealth index were found to be associated with the time since last dental care visit.
En Perú, los adultos mayores presentan un bajo uso de los servicios odontológicos, además de percibirse una gradiente social para su utilización. Adicionalmente, la pandemia por COVID-19 desencadenó escenarios complejos, caracterizados por un inadecuado acceso a los servicios de salud, que afectó con mayor énfasis a grupos poblacionales vulnerables, como las personas en edades extremas. El objetivo de este estudio fue determinar el impacto de la pandemia por COVID-19 en el tiempo de atención odontológica en adultos mayores peruanos. Estudio transversal con población de 5247 encuestados para el 2019 y 5066 para el 2020. Se consideraron los registros de quienes respondieron a la pregunta sobre el tiempo desde su última atención odontológica, extrayéndose del 2019 un total de 4045 y para 2020, 3943 sujetos. La variable dependiente consistió en el tiempo desde la última atención odontológica medido en años; mientras que dentro de tres dimensiones,seagruparon a lasvariables sobre características de salud, geográficas y sociodemográficas. Se aplicó un análisis descriptivo, bivariado y multivariado, donde el tiempo desde la última atención odontológica en el año 2019 fue de 7.93 años (DE=8.03) y en el 2020 fue de 7.94 años (DE=7.28). Se realizó un análisis de regresión lineal múltiple jerárquica, donde ninguna variable del modelo 2019 fue significativa; mientras que para 2020, las variables características de salud, geográficas y sociodemográficas si lo fueron. Al análisis, donde el año pasa a ser independiente, sólo el modelo 4, que considera todas las variables fue válido (p=0.018); asimismo, el área de residencia e índice de riqueza presentaron significancia estadística. Para concluir, en el año 2020 la pandemia por COVID-19 no impactó en el tiempo de atención odontológica en adultos mayores peruanos, sin embargo, factores como área de residencia e índice de riqueza si presentaron asociación con el tiempo desdelaúltimaatención.
Assuntos
COVID-19 , Idoso , COVID-19/epidemiologia , Estudos Transversais , Assistência Odontológica , Humanos , Pandemias , Peru/epidemiologiaRESUMO
ABSTRACT In Peru, there is low dental service use among older adults, in addition to a social gradient for use. Furthermore, the COVID-19 pandemic triggered complex scenarios characterized by inadequate access to health services, with greater impact on vulnerable population groups such as the elderly. The aim of this study was to determine the impact of the COVID-19 pandemic on the time since last dental care visit among elderly Peruvians. It was a cross-sectional study conducted on a population of 5247 respondents in 2019, and 5066 respondents in 2020. The study considered the answers of people who responded to the question on the last time they had received dental care, extracting a total 4045 subjects for 2019 and 3943 for 2020. The dependent variable was time since last dental care measured in years, while the variables on health, geographic and sociodemographic characteristics were grouped within three dimensions. A descriptive bivariate and multivariate analysis was applied by means of multiple linear regression to analyze the behavior of the variables. Time since the last dental care visit was 7.93 years (SD=8.03) in 2019 and 7.94 years (SD=7.28) in 2020. A hierarchical multiple linear regression analysis was performed, where no variable in the 2019 model was significant; while for 2020, health, geographic, and sociodemographic characteristics variables were significant. In the analysis where the 'year' variable becomes independent, only model 4, which considers all variables, was valid (p=0.018). The variables 'area of residence' and 'wealth index' were also significant. To conclude, the 2020 pandemic year for COVID-19 had no impact on the time since last dental care visit among elderly Peruvians, though factors such as area of residence and wealth index were found to be associated with the time since last dental care visit.
RESUMEN En Perú, los adultos mayores presentan un bajo uso de los servicios odontológicos, además de percibirse una gradiente social para su utilización. Adicionalmente, la pandemia por COVID-19 desencadenó escenarios complejos, caracterizados por un inadecuado acceso a los servicios de salud, que afectó con mayor énfasis a grupos poblacionales vulnerables, como las personas en edades extremas. El objetivo de este estudio fue determinar el impacto de la pandemia por COVID-19 en el tiempo de atención odontológica en adultos mayores peruanos. Estudio transversal con población de 5247 encuestados para el 2019 y 5066 para el 2020. Se consideraron los registros de quienes respondieron a la pregunta sobre el tiempo desde su última atención odontológica, extrayéndose del 2019 un total de 4045 y para 2020, 3943 sujetos. La variable dependiente consistió en el tiempo desde la última atención odontológica medido en años; mientras que dentro de tres dimensiones, se agruparon a las variables sobre características de salud, geográficas y sociodemográficas. Se aplicó un análisis descriptivo, bivariado y multivariado, donde el tiempo desde la última atención odontológica en el año 2019 fue de 7.93 años (DE=8.03) y en el 2020 fue de 7.94 años (DE=7.28). Se realizó un análisis de regresión lineal múltiple jerárquica, donde ninguna variable del modelo 2019 fue significativa; mientras que para 2020, las variables características de salud, geográficas y sociodemográficas si lo fueron. Al análisis, donde el año pasa a ser independiente, sólo el modelo 4, que considera todas las variables fue válido (p=0.018); asimismo, el área de residencia e índice de riqueza presentaron significancia estadística. Para concluir, en el año 2020 la pandemia por COVID-19 no impactó en el tiempo de atención odontológica en adultos mayores peruanos, sin embargo, factores como área de residencia e índice de riqueza si presentaron asociación con el tiempo desde la última atención.
RESUMO
The Lambda variants of interest (VOI) (C37/GR/452Q.V1/21G) was initially reported in Lima, Peru but has gained rapid dissemination through other Latin American countries. Nevertheless, the dissemination and molecular epidemiology of the Lambda VOI in Brazil is unknown apart from a single case report. In this respect, we characterized the circulation of the SARS-CoV-2 Lambda VOI (C37/GR/452Q.V1/21G) in Sao Paulo State, Brazil. From March to June 2021, we identified seven Lambda isolates in a set of approximately 8000 newly sequenced genomes of the Network for Pandemic Alert of Emerging SARS-CoV-2 variants from Sao Paulo State. Interestingly, in three of the positive patients, the Lambda VOI infection was probably related to a contact transmission. These individuals were fully vaccinated to COVID-19 and presented mild symptoms. The remaining positive for Lambda VOI individuals showed different levels of COVID-19 symptoms and one of them needed hospitalization (score 5, WHO). In our study, we present a low level of Lambda VOI circulation in the Sao Paulo State. This reinforces the essential role of molecular surveillance for the effective SARS-CoV-2 pandemic response, especially in regard to circulating variants.
Assuntos
COVID-19 , SARS-CoV-2 , Brasil/epidemiologia , COVID-19/epidemiologia , Humanos , SARS-CoV-2/genética , Organização Mundial da SaúdeRESUMO
Sao Paulo State, currently experiences a second COVID-19 wave overwhelming the healthcare system. Due to the paucity of SARS-CoV-2 complete genome sequencing, we established a Network for Pandemic Alert of Emerging SARS-CoV-2 Variants to rapidly understand and monitor the spread of SARS-CoV-2 variants into the state. Through analysis of 210 SARS-CoV-2 complete genomes obtained from the largest regional health departments we identified cocirculation of multiple SARS-CoV-2 lineages such as B.1.1 (0.5%), B.1.1.28 (23.2%), B.1.1.7 (alpha variant, 6.2%), B.1.566 (1.4%), B.1.544 (0.5%), C.37 (0.5%) P.1 (gamma variant, 66.2%), and P.2 (zeta variant, 1.0%). Our analysis allowed also the detection, for the first time in Brazil, the South African B.1.351 (beta) variant of concern, B.1.351 (501Y.V2) (0.5%), characterized by the following mutations: ORF1ab: T265I, R724K, S1612L, K1655N, K3353R, SGF 3675_F3677del, P4715L, E5585D; spike: D80A, D215G, L242_L244del, A262D, K417N, E484K, N501Y, D614G, A701V, C1247F; ORF3a: Q57H, S171L, E: P71L; ORF7b: Y10F, N: T205I; ORF14: L52F. The most recent common ancestor of the identified strain was inferred to be mid-October to late December 2020. Our analysis demonstrated the P.1 lineage predominance and allowed the early detection of the South African strain for the first time in Brazil. We highlight the importance of SARS-CoV-2 active monitoring to ensure the rapid detection of potential variants for pandemic control and vaccination strategies. Highlights Identification of B.1.351 (beta) variant of concern in the Sao Paulo State. Dissemination of SARS-CoV-2 variants of concern and interest in the Sao Paulo State. Mutational Profile of the circulating variants of concern and interest.
Assuntos
SARS-CoV-2/genética , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/imunologia , Brasil , COVID-19/imunologia , COVID-19/virologia , Genômica/métodos , Humanos , Mutação/genética , Mutação/imunologia , SARS-CoV-2/imunologia , Glicoproteína da Espícula de Coronavírus/genética , Glicoproteína da Espícula de Coronavírus/imunologiaRESUMO
Trypanosoma cruzi is a protozoan parasite belonging to the Trypanosomatidae family. Although the trypanosomatids multiply predominantly by clonal generation, the presence of DNA exchange in some of them has been puzzling researchers over the years, mainly because it may represent a novel form that these organisms use to gain variability. Analysis of DNA Exchange using Thymidine Analogs (ADExTA) is a method that allows the in vitro detection and measurement of rates of DNA exchange, particularly in trypanosomatid cells, in a rapid and simple manner by indirect immunofluorescence assay (IFA). The method can be used to detect DNA exchange within one trypanosomatid lineage or among different lineages by paired analysis. The principle of this assay is based on the incorporation of two distinguishable halogenated thymidine analogs called 5'-chloro-2'-deoxyuridine (CldU) and 5'-iodo-2'-deoxyuridine (IdU) during DNA replication. After mixing the two cell cultures that had been previously incorporated with CldU and IdU separately, the presence of these unusual deoxynucleosides in the genome can be detected by specific antibodies. For this, a DNA denaturation step is required to expose the sites of thymidine analogs incorporated. Subsequently, a secondary reaction using fluorochrome-labeled antibodies will generate distinct signals under fluorescence analysis. By using this method, DNA exchange verification (i.e., the presence of both CldU and IdU in the same cell) is possible using a standard fluorescence microscope. It typically takes 2-3 days from the thymidine analogs incorporation to results. Of note, ADExTA is relatively cheap and does not require transfections or harsh genetic manipulation. These features represent an advantage when compared to other time-consuming protocols that demand DNA manipulation to introduce distinct drug-resistance markers in different cells for posterior selection.
RESUMO
Mutualism is defined as a beneficial relationship for the associated partners and usually assumes that the symbiont number is controlled. Some trypanosomatid protozoa co-evolve with a bacterial symbiont that divides in coordination with the host in a way that results in its equal distribution between daughter cells. The mechanism that controls this synchrony is largely unknown, and its comprehension might provide clues to understand how eukaryotic cells evolved when acquiring symbionts that later became organelles. Here, we approached this question by studying the effects of inhibitors that affect the host exclusively in two symbiont-bearing trypanosomatids, Strigomonas culicis and Angomonas deanei. We found that inhibiting host protein synthesis using cycloheximide or host DNA replication using aphidicolin did not affect the duplication of bacterial DNA. Although the bacteria had autonomy to duplicate their DNA when host protein synthesis was blocked by cycloheximide, they could not complete cytokinesis. Aphidicolin promoted the inhibition of the trypanosomatid cell cycle in the G1/S phase, leading to symbiont filamentation in S. culicis but not in A. deanei. Treatment with camptothecin blocked the host protozoa cell cycle in the G2 phase and induced the formation of filamentous symbionts in both species. Oryzalin, which affects host microtubule polymerization, blocked trypanosomatid mitosis and abrogated symbiont division. Our results indicate that host factors produced during the cell division cycle are essential for symbiont segregation and may control the bacterial cell number.
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OBJECTIVE: To compare the lipid profiles and coronary heart disease risks of 2 Brazilian Amazonian populations as follows: a riverside population (village of Vigia) and an urban population (city of Belém in the state of Pará). METHODS: Fifty individuals controlled for age and sex were assessed in each region, and the major risk factors for coronary heart disease were analyzed. RESULTS: According to the National Cholesterol Education Program (NCEP III) and using the Framingham score, both populations had the same absolute risk of events (Vigia = 5.4 ± 1 vs Belém = 5.7 ± 1), although the population of Vigia had a lower consumption of saturated fat (P<0.0001), a greater consumption of mono- and polyunsaturated fat (P<0.03), in addition to lower values for body mass index (25.4± 0.6 vs 27.6 ± 0.7 kg/m², P<0.02), of biceps skin fold (18.6 ± 1.1 vs 27.5 ± 1.3 mm, P<0.0001), of triceps skin fold (28.7 ± 1.2 vs 37.3 ± 1.7 mm, P<0.002), and of total cholesterol (205 ± 5 vs 223 ± 6 mg/dL, P< 0.03) and triglycerides (119 ± 9 vs 177 ± 18 mg/dL, P<0.005). Both populations did not differ in regard to HDL-C (46 ± 1 vs 46 ± 1 mg/dL), LDL-C (135 ± 4 vs 144 ± 5 mg/dL) and blood pressure (SBP 124 ± 3 vs 128 ± 3 mmHg; DBP 80 ± 2 vs 82 ± 2 mmHg). CONCLUSION: The riverside and urban populations of Amazonia had similar cardiovascular risks. However, the marked difference in the variables studied suggests that different strategies of prevention should be applied
Assuntos
Humanos , Animais , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Doenças Cardiovasculares , Gorduras na Dieta , Lipídeos , Antropometria , Brasil , Doenças Cardiovasculares , Distribuição de Qui-Quadrado , Colesterol , Dieta , Ácidos Graxos Monoinsaturados , Ácidos Graxos Insaturados , Peixes , Fatores de Risco , População Rural , Fumar , Triglicerídeos , População UrbanaRESUMO
OBJECTIVE: To compare the lipid profiles and coronary heart disease risks of 2 Brazilian Amazonian populations as follows: a riverside population (village of Vigia) and an urban population (city of Bel m in the state of Par ). METHODS: Fifty individuals controlled for age and sex were assessed in each region, and the major risk factors for coronary heart disease were analyzed. RESULTS: According to the National Cholesterol Education Program (NCEP III) and using the Framingham score, both populations had the same absolute risk of events (Vigia = 5.4 +/- 1 vs Bel m = 5.7 +/- 1), although the population of Vigia had a lower consumption of saturated fat (P<0.0001), a greater consumption of mono- and polyunsaturated fat (P<0.03), in addition to lower values for body mass index (25.4 +/- 0.6 vs 27.6 +/- 0.7 kg/m , P<0.02), of biceps skin fold (18.6 1.1 vs 27.5 +/- 1.3 mm, P<0.0001), of triceps skin fold (28.7 +/- 1.2 vs 37.3 +/- 1.7 mm, P<0.002), and of total cholesterol (205 +/- 5 vs 223 +/- 6 mg/dL, P< 0.03) and triglycerides (119 +/- 9 vs 177 +/- 18 mg/dL, P<0.005). Both populations did not differ in regard to HDL-C (46 +/- 1 vs 46 +/- 1 mg/dL), LDL-C (135 +/- 4 vs 144 +/- 5 mg/dL) and blood pressure (SBP 124 +/- 3 vs 128 +/- 3 mmHg; DBP 80 +/- 2 vs 82 +/- 2 mmHg). CONCLUSION: The riverside and urban populations of Amazonia had similar cardiovascular risks. However, the marked difference in the variables studied suggests that different strategies of prevention should be applied.
Assuntos
Doenças Cardiovasculares/etiologia , Gorduras na Dieta/administração & dosagem , Lipídeos/sangue , Adulto , Idoso , Brasil/epidemiologia , Doenças Cardiovasculares/epidemiologia , Distribuição de Qui-Quadrado , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , População Rural , População UrbanaRESUMO
Marco de referencia : Un gran hospital público en Buenos Aires, Argentina. Objetivo: Se efectuó un estudio en 155 pacientes VIH positivos ingresados al Hospital de Agudos "Dr. Enrique Tornú" entre 1992 y 1996 para conocer la distribución de las enfermedades respiratorias. Métodos : Se estudiaron los diagnósticos clínicos, los gérmenes causales, la sensibilidad de estos últimos a los antibióticos, el conteo de linfocitos T CD4 y CD8 y la mortalidad intrahospitalaria de 155 pacientes varones con un test VIH positivo en el Servicio de Tisioneumonología. Actuaron como grupo control 130 pacientes varones, VIH negativos, seleccionados al azar. Resultados: Los 155 pacientes VIH positivos tenían una edad media de 32,3ñ8,4 años y los 130 pacientes VIH negativos 37,2ñ13,2 años. Se diagnosticaron en los 155 pacientes VIH positivos las siguientes enfermedades: tuberculosis (TB) en 103 casos (66,4 por ciento), neumonías de la comunidad (NC) en 35 (22,5 por ciento), neumonía por Pneumocystis carinii (PCP) en 19 (12,2 por ciento) candidiasis en 41 (26,4 por ciento) y otras enfermedades en el resto. En el grupo VIH negativo hubo 66 TB (50,7 por ciento), 13 EPOC (10 por ciento), 11 NC (8,4 por ciento) y otros procesos en el resto. De los 155 pacientes VIH positivos murieron en el hospital 45 (29 por ciento) de: TB 31 (39 por ciento), de NC 7 (20 por ciento), de NPC 4 (44,4 por ciento), de criptococcosis 2 (20 por ciento), de Kaposi 1. Conclusión : En Buenos Aires, entre los varones VIH positivos la tuberculosis es la complicación respiratoria más frecuente (66,4 por ciento), seguida por la neumonía de la comunidad (25 por ciento) y la neumonía por Pneumocystis carinii (12,2 por ciento). Implicaciones clínicas : El test de VIH se debe llevar a cabo en todos los pacientes entre 15 y 60 años de edad con una TB confirmada o sospechosa o una neumonía; para los pacientes VIH positivos el conteo de CD4 es importante debido a su valor pronóstico.