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Objetivo: analisar diagnósticos e tratamentos em pacientes no Hospital do Juquery no período Vargas. Método: estudo quanti-qualitativo, exploratório-descritivo, análise dos dados pelo referencial da análise documental e documentos históricos. Resultados:analisados 2.166 prontuários; épocas de conflitos sociais e guerras com incremento de prisões e internações; múltiplos diagnósticos para paciente; diagnósticos inconclusivos; principais diagnósticos.: esquizofrenia (23,59%), psicose maníaca depressiva (4,20%), psicoses diversas (4,02%), delírio (3,83%), parafrenia (3,60%), depressão (3,19%), confusão mental (2,91%) configuravam 45,34% (982) do total; quadros sem serem estritamente psiquiátricos, mas poderiam ter sintomatologia derivada.: sífilis (10,80%), deficiência intelectual (8,08%), epilepsia (4,06%), alcoolismo (3,81%); 2.023 (93,40%) prontuários sem observação médica; 08 (0,37%) pacientes diagnosticados "sem perturbação mental"; 37 (1,71%) "sem diagnóstico conclusivo"; 920 (42,47%) prontuários sem dados de tratamentos; 213 (9,83%) sem definição de tratamentos. Conclusão: múltiplos diagnósticos para mesmo indivíduo; alguns pacientes não tinham quadros psiquiátricos; tratamentos repetidos, majoritariamente orgânicos, não produziram resultados efetivos
Objective: to analyze diagnoses and treatments in patients at the Juquery Hospital during the Vargas period. Method: a quantitative-qualitative, exploratory-descriptive study, analyzing the data using documentary analysis and historical documents. Results: 2. 166 medical records analyzed; times of social conflict and war with an increase in arrests and hospitalizations; multiple diagnoses for patients; inconclusive diagnoses; main diagnoses: schizophrenia (23.59%), manic depressive psychosis (4.20%), various psychoses (4.02%), delirium (3.83%), paraphrenia (3.60%), depression (3.19%), mental confusion (2.91%) made up 45.34% (982) of the total; conditions that were not strictly psychiatric, but could have derived symptoms: syphilis (10.80%), intellectual disability (8.08%), epilepsy (4.06%), alcoholism (3.81%); 2. 023 (93.40%) medical records without medical observation; 08 (0.37%) patients diagnosed "without mental disorder"; 37 (1.71%) "without conclusive diagnosis"; 920 (42.47%) medical records without treatment data; 213 (9.83%) without treatment definition. Conclusion: multiple diagnoses for the same individual; some patients did not have psychiatric conditions; repeated treatments, mostly organic, did not produce effective results
Objetivos:analizar los diagnósticos y tratamientos de los pacientes del Hospital de Juquery durante el período de Vargas. Método:estudio cuantitativo-cualitativo, exploratorio-descriptivo, analizando los datos mediante análisis documental y documentos históricos. Resultados: 2. 166 historias clínicas analizadas; época de conflicto social y guerra con aumento de detenciones y hospitalizaciones; diagnósticos múltiples para los pacientes; diagnósticos no concluyentes; principales diagnósticos: esquizofrenia (23,59%), psicosis maníaco depresiva (4,20%), psicosis diversas (4,02%), delirio (3,83%), parafrenia (3,60%), depresión (3,19%), confusión mental (2,91%) constituyeron el 45,34% (982) del total; afecciones no estrictamente psiquiátricas, pero que podían tener síntomas derivados: sífilis (10,80%), discapacidad intelectual (8,08%), epilepsia (4,06%), alcoholismo (3,81%); 2. 023 (93,40%) historias clínicas sin observación médica; 08 (0,37%) pacientes diagnosticados "sin trastorno mental"; 37 (1,71%) "sin diagnóstico concluyente"; 920 (42,47%) historias clínicas sin datos de tratamiento; 213 (9,83%) sin definición de tratamiento. Conclusión: múltiples diagnósticos para el mismo individuo; algunos pacientes no tenían afecciones psiquiátricas; los tratamientos repetidos, en su mayoría orgánicos, no produjeron resultados efectivos
Assuntos
Humanos , Masculino , Feminino , Saúde Mental , Saúde Pública/história , Hospitais PsiquiátricosRESUMO
Background and aims: Latin American populations remain underrepresented in genetic studies of inflammatory bowel diseases (IBDs). Most genetic association studies of IBD rely on Caucasian, African, and Asian individuals. These associations have yet to be evaluated in detail in the Andean region of South America. We explored the contribution of IBD-reported genetic risk variants to a Chilean cohort and the ancestry contribution to IBD in this cohort. Methods: A total of 192 Chilean IBD patients were genotyped using Illumina's Global Screening Array. Genotype data were combined with similar information from 3,147 Chilean controls. The proportions of Aymara, African, European, and Mapuche ancestries were estimated using the software ADMIXTURE. We calculated the odds ratios (ORs) and 95% confidence intervals (CIs) for gender, age, and ancestry proportions. We also explored associations with previously reported IBD-risk variants independently and in conjunction with genetic ancestry. Results: The first and third quartiles of the proportion of Mapuche ancestry in IBD patients were 24.7 and 34.2%, respectively, and the corresponding OR was 2.30 (95%CI 1.52-3.48) for the lowest vs. the highest group. Only one variant (rs7210086) of the 180 reported IBD-risk SNPs was associated with IBD risk in the Chilean cohort (adjusted P = 0.01). This variant is related to myeloid cells. Conclusion: The type and proportion of Native American ancestry in Chileans seem to be associated with IBD risk. Variants associated with IBD risk in this Andean region were related to myeloid cells and the innate immune response.
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Lactose intolerance (LI) and vitamin D deficiency (VDD) have been linked to inflammatory bowel disease (IBD). We conducted an observational study in 192 Chilean IBD patients to investigate the prevalence of a specific gene variant (LCT-13910 CC genotype) associated with LI and the prevalence of VDD/Vitamin D Receptor (VDR) gene variants. Blood samples were analyzed using Illumina's Infinium Global Screening Array. The LCT-13910 CC genotype was found in 61% of IBD patients, similar to Chilean Hispanic controls and lower than Chilean Amerindian controls. The frequency of the LCT-13910-C allele in Chilean IBD patients (0.79) was comparable to the general population and higher than Europeans (0.49). Regarding VDR and VDD variants, in our study, the rs12785878-GG variant was associated with an increased risk of IBD (OR = 2.64, CI = 1.61-4.32; p-value = 0.001). Sixty-one percent of the Chilean IBD cohort have a genetic predisposition to lactose malabsorption, and a significant proportion exhibit genetic variants associated with VDD/VDR. Screening for LI and VDD is crucial in this Latin American IBD population.
Assuntos
Doenças Inflamatórias Intestinais , Lactose , Receptores de Calcitriol , Humanos , Chile/epidemiologia , Predisposição Genética para Doença , Genótipo , Doenças Inflamatórias Intestinais/epidemiologia , Doenças Inflamatórias Intestinais/genética , Doenças Inflamatórias Intestinais/complicações , Lactose/deficiência , Polimorfismo de Nucleotídeo Único , Prevalência , Receptores de Calcitriol/genética , Vitamina D , Deficiência de Vitamina D/complicações , Deficiência de Vitamina D/epidemiologia , Deficiência de Vitamina D/genéticaRESUMO
Objetivo: analisar os tratamentos e desfechos nos internados no Hospital do Juquery no período de 1930 a 1945. Métodos: estudo quanti-qualitativo, exploratório-descritivo, análise dos dados pelo referencial da análise documental. Coleta dos dados foi realizada no Acervo do Patrimônio Histórico-cultural do Complexo Hospitalar do Juquery, entre março e julho de 2022.Resultados: analisados 2.166 prontuários; 920 prontuários sem dados de tratamentos; 213 sem definição de tratamentos; principais tipos de tratamento 494 monoterapia e 235 politerapia; 2.005 prontuários sem dados dos efeitos dos tratamentos. Quanto ao resultado 106 inalterado/não melhorou, 21 melhorou, 18 piorou, 16 morte súbita. Houve 366 desfechos sem dados, 868 óbitos dos quais 496 não especificados, 263 saídas sem alta, 365 saídas com alta, evadidos 36 e 252 transferidos para outras instituições psiquiátricas. Conclusão: os tratamentos eram majoritariamente orgânicos e não produziram resultados efetivos; desfechos apontam para inadequação e ineficiência da assistência psiquiátrica e da internação.
Objective: to analyze the treatments and outcomes of patients admitted to the Juquery Hospital from 1930 to 1945. Methods: quanti-qualitative, exploratory-descriptive study, data analysis by the reference of documentary analysis. Data collection was carried out in the Historical-Cultural Heritage Collection of the Juquery Hospital Complex, between March and July 2022. Results: 2,166 medical records were analyzed; 920 medical records without treatment data; 213 without definition of treatments; main types of treatment: 494 monotherapy and 235 polytherapy; 2,005 medical records without data on the effects of treatments. Regarding outcome: 106 unchanged/not improved, 21 improved, 18 worsened, 16 sudden death. There were 366 outcomes without data, 868 deaths of which 496 unspecified, 263 discharged, 365 discharged, 36 absconded and 252 transferred to other psychiatric institutions. Conclusion: treatments were mostly organic and did not produce effective results; outcomes point to inadequacy and inefficiency of psychiatric care and hospitalization.
Objetivos:analizar los tratamientos y resultados de los pacientes ingresados en el Hospital de Juquery de 1930 a 1945. Métodos: estudio cuantitativo-cualitativo, exploratorio-descriptivo, análisis de datos por la referencia del análisis documental. La recolección de datos se realizó en la Colección de Patrimonio Histórico-Cultural del Complejo Hospitalario Juquery, entre marzo y julio de 2022. Resultados: se analizaron 2.166 historias clínicas; 920 historias clínicas sin datos de tratamiento; 213 sin definición de tratamientos; principales tipos de tratamiento: 494 monoterapia y 235 politerapia; 2.005 historias clínicas sin datos sobre los efectos de los tratamientos. En cuanto al resultado: 106 sin cambios/no mejoría, 21 mejoría, 18 empeoramiento, 16 muerte súbita. Hubo 366 desenlaces sin datos, 868 fallecimientos de los cuales 496 sin especificar, 263 dados de alta, 365 dados de alta, 36 fugados y 252 trasladados a otras instituciones psiquiátricas. Conclusión: los tratamientos fueron en su mayoría orgánicos y no produjeron resultados eficaces; los resultados apuntan a la inadecuación e ineficacia de la atención psiquiátrica y la hospitalización.
Assuntos
Psiquiatria/história , Prontuários Médicos/estatística & dados numéricos , Hospitais Psiquiátricos/estatística & dados numéricos , Assistência à Saúde MentalRESUMO
Abstract Mental health problems in the general population tend to be related to social determinants which also influence health inequity. However, these determinants are usually taken into account only for clinical populations and often go unnoticed at the community level. The purpose of the present study was to identify the psycho-socio-cultural factors that influence the presence of psychopathological symptoms in the open population. In this study, 229 women and men, Mexican adults, participated. Two self-report instruments were used: the SCL 90-R to evaluate psychopathological symptoms, and the Questionnaire of Factors Associated with Health. The results show differences between men and women, both in the symptoms and in the factors evaluated, where women are more disadvantaged. Predictive models indicate that in women the factors predicting the symptomatology are: conflicting thoughts, domestic violence, lack of confidence in their abilities and gender discrimination; while in men they were: family violence, poverty and lower well-being. It is concluded that psychosocial and ecosystemic models enable to understand that psycho-socio-cultural factors influence the presence of incipient symptoms in the community population, following different paths for women and men, showing that the context has a differential impact on mental health.
Resumen Los problemas de salud mental en la población general tienden a estar relacionados con determinantes sociales que también influyen en la inequidad sanitaria. Sin embargo, estos determinantes suelen tenerse en cuenta solo en la población clínica y pasan inadvertidos en el ámbito comunitario. Teniendo esto en cuenta, el propósito del presente estudio fue identificar los factores psicosocioculturales que influyen en la presencia de los síntomas psicopatológicos en población abierta. Para esto, participaron 229 mujeres y hombres, adultos, mexicanos, a quienes se les aplicaron dos instrumentos de autorreporte para evaluar sus síntomas psicopatológicos, el SCL 90-R y el Cuestionario de Factores Asociados a la Salud. Los resultados muestran diferencias entre hombres y mujeres tanto en los síntomas como en los factores evaluados, en donde las mujeres resultaron ser las más desfavorecidas. Los modelos predictivos señalan que en las mujeres los factores que predicen la sintomatología son los pensamientos conflictivos, la violencia intrafamiliar, la falta de confianza en sus capacidades y la discriminación de género; mientras que en los hombres fueron la violencia intrafamiliar, la pobreza y un menor bienestar. Se concluye que los modelos psicosociales y ecosistémicos permiten comprender que los factores psicosocioculturales influyen sobre los síntomas incipientes de población comunitaria, con vías diferentes tanto para las mujeres como para los hombres. Con esto se demuestra que el contexto imprime un impacto diferencial en la salud mental.
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Protein malnutrition is a risk factor for developing visceral leishmaniasis. Because we previously demonstrated that protein malnutrition and infection with Leishmania infantum disrupts the splenic microarchitecture in BALB/c mice, alters T cell-subsets and increases splenic parasite load, we hypothesize that splenic microenvironment is precociously compromised in infected animals that suffered a preceding malnutrition. To evaluate this, we characterized the abundance of proteins secreted in the splenic interstitial fluid (IF) using an iTRAQ-based quantitative proteomics approach. In addition, local levels of pro-inflammatory and proliferation molecules were analyzed. Whereas well-nourished infected animals showed increased IL-1ß and IL-2 levels, malnourished-infected mice displayed significant reduction of these cytokines. Remarkably, a two-weeks infection with L. infantum already modified protein abundance in the splenic IF of well-nourished mice, but malnourished animals failed to respond to infection in the same fashion. Malnutrition induced significant reduction of chemotactic and pro-inflammatory molecules as well as of proteins involved in nucleic acid and amino acid metabolism, indicating an impaired proliferative microenvironment. Accordingly, a significant decrease in Ki67 expression was observed, suggesting that splenocyte proliferation is compromised in malnourished animals. Together, our results show that malnutrition compromises the splenic microenvironment and alters the immune response to the parasite in malnourished individuals. SIGNIFICANCE: Protein malnutrition is recognized as an important epidemiological risk factor for developing visceral leishmaniasis (VL). Locally secreted factors present in the interstitial fluid have important roles in initiating immune responses and in regulating fluid volume during inflammation. However, the regulation of secreted factors under pathological conditions such as malnutrition and infection are widely unknown. To analyze how protein malnutrition alters secreted proteins involved in the immune response to L. infantum infection we evaluated the proteomic profile of the interstitial fluid of the spleen in malnourished BALB/c mice infected with L. infantum. Our work revealed new elements that contribute to the understanding of the immunopathological events in the spleen of malnourished animals infected with L. infantum and opens new pathways for consideration of other aspects that could improve VL treatment in malnourished individuals.
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Proliferação de Células , Líquido Extracelular/metabolismo , Perfilação da Expressão Gênica , Leishmania infantum/metabolismo , Leishmaniose Visceral/metabolismo , Desnutrição/metabolismo , Proteômica , Baço/metabolismo , Animais , Líquido Extracelular/parasitologia , Inflamação/metabolismo , Inflamação/parasitologia , Inflamação/patologia , Leishmaniose Visceral/patologia , Masculino , Desnutrição/parasitologia , Desnutrição/patologia , Camundongos , Camundongos Endogâmicos BALB C , Baço/parasitologia , Baço/patologiaRESUMO
Detrimental effects of malnutrition on immune responses to pathogens have long been recognized and it is considered a main risk factor for various infectious diseases, including visceral leishmaniasis (VL). Thymus is a target of both malnutrition and infection, but its role in the immune response to Leishmania infantum in malnourished individuals is barely studied. Because we previously observed thymic atrophy and significant reduction in cellularity and chemokine levels in malnourished mice infected with L. infantum, we postulated that the thymic microenvironment is severely compromised in those animals. To test this, we analyzed the microarchitecture of the organ and measured the protein abundance in its interstitial space in malnourished BALB/c mice infected or not with L. infantum. Malnourished-infected animals exhibited a significant reduction of the thymic cortex:medulla ratio and altered abundance of proteins secreted in the thymic interstitial fluid. Eighty-one percent of identified proteins are secreted by exosomes and malnourished-infected mice showed significant decrease in exosomal proteins, suggesting that exosomal carrier system, and therefore intrathymic communication, is dysregulated in those animals. Malnourished-infected mice also exhibited a significant increase in the abundance of proteins involved in lipid metabolism and tricarboxylic acid cycle, suggestive of a non-proliferative microenvironment. Accordingly, flow cytometry analysis revealed decreased proliferation of single positive and double positive T cells in those animals. Together, the reduced cortical area, decreased proliferation, and altered protein abundance suggest a dysfunctional thymic microenvironment where T cell migration, proliferation, and maturation are compromised, contributing for the thymic atrophy observed in malnourished animals. All these alterations could affect the control of the local and systemic infection, resulting in an impaired response to L. infantum infection.
Assuntos
Interações Hospedeiro-Patógeno/imunologia , Leishmania infantum/imunologia , Leishmaniose Visceral/imunologia , Desnutrição/imunologia , Linfócitos T/imunologia , Timo/imunologia , Animais , Transporte Biológico , Movimento Celular , Proliferação de Células , Ciclo do Ácido Cítrico/genética , Ciclo do Ácido Cítrico/imunologia , Exossomos/imunologia , Exossomos/metabolismo , Exossomos/parasitologia , Líquido Extracelular/imunologia , Líquido Extracelular/metabolismo , Líquido Extracelular/parasitologia , Galectina 1/genética , Galectina 1/imunologia , Regulação da Expressão Gênica , Interações Hospedeiro-Patógeno/genética , Imunidade Inata , Leishmania infantum/crescimento & desenvolvimento , Leishmaniose Visceral/genética , Leishmaniose Visceral/metabolismo , Leishmaniose Visceral/parasitologia , Metabolismo dos Lipídeos , Masculino , Desnutrição/genética , Desnutrição/metabolismo , Desnutrição/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Plasminogênio/genética , Plasminogênio/imunologia , Proteoma/genética , Proteoma/imunologia , Linfócitos T/parasitologia , Timo/metabolismo , Timo/parasitologiaRESUMO
Los problemas de salud mental afectan el desarrollo biopsicosocial de las personas. Dichos problemas empeoran debido a que se acude tardíamente a los servicios de salud, evidenciando la necesidad de detecciones tempranas en población abierta. Por ello, el objetivo de esta investigación fue evaluar la relación entre síntomas psicopatológicos y calidad de vida en mujeres y hombres. Participaron 465 personas, habitantes del Estado de México, quienes contestaron el SCL-90-R y el WHOQOL-BREF. En los resultados, las mujeres presentan mayores puntuaciones de síntomas psicopatológicos y menor calidad de vida con respecto a los hombres. Los problemas de salud mental afectan la calidad de vida por diferentes vías de influencia de acuerdo al sexo; en las mujeres destaca la depresión y la sintomatología global; y en los hombres, la somatización, la sintomatología global y la depresión. Se discute sobre la necesidad de prevención y detección precoz de los síntomas incipientes a través del trabajo comunitario.
Mental health problems, which affect biopsychosocial development of people, worsen because people take too long to come to health services. This situation shows the need for early detection of these problems in open populations. Considering this, the present research aimed to assess the relationship between psychopathological symptoms and quality of life in women and men. The study population consisted of 465 inhabitants of the State of Mexico, who answered the SCL-90-R and WHOQOLBREF questionnaires. Results: women have higher scores of psychopathological symptoms and lower quality of life than men. Mental health problems affect quality of life in different ways according to gender: in women, depression and global symptoms stand out; and in men, somatization, global symptoms and depression are the most common ones. This paper discusses the need for prevention and early detection of incipient symptoms of mental health problems through community work.
RESUMO
Protein malnutrition, the most deleterious cause of malnutrition in developing countries, has been considered a primary risk factor for the development of clinical visceral leishmaniasis (VL). Protein malnutrition and infection with Leishmania infantum leads to lymphoid tissue disorganization, including changes in cellularity and lymphocyte subpopulations in the thymus and spleen. Here we report that protein malnutrition modifies thymic chemotactic factors by diminishing the CCL5, CXCL12, IGF1, CXCL9 and CXCL10 protein levels in infected animals. Nevertheless, T cells preserve their migratory capability, as they were able to migrate ex vivo in response to chemotactic stimuli, indicating that malnutrition may compromise the thymic microenvironment and alter in vivo thymocyte migration. Decrease in chemotactic factors protein levels was accompanied by an early increase in the parasite load of the spleen. These results suggest that the precondition of malnutrition is affecting the cell-mediated immune response to L. infantum by altering T cell migration and interfering with the capacity of protein-deprived animals to control parasite spreading and proliferation. Our data provide evidence for a disturbance of T lymphocyte migration involving both central and peripheral T-cells, which likely contribute to the pathophysiology of VL that occurs in malnourished individuals.
Assuntos
Movimento Celular , Leishmania infantum/patogenicidade , Leishmaniose Visceral/complicações , Leishmaniose Visceral/imunologia , Desnutrição/complicações , Desnutrição/imunologia , Linfócitos T/patologia , Timo/patologia , Animais , Apoptose , Atrofia , Peso Corporal , Quimiotaxia , Citocinas/sangue , Fator de Crescimento Insulin-Like I/metabolismo , Leishmaniose Visceral/sangue , Leishmaniose Visceral/parasitologia , Leptina/sangue , Ligantes , Macrófagos/metabolismo , Macrófagos/patologia , Desnutrição/sangue , Desnutrição/parasitologia , Camundongos Endogâmicos BALB C , Carga Parasitária , Parasitos/patogenicidade , Receptores CXCR3/metabolismo , Baço/parasitologia , Timócitos/patologiaRESUMO
Leishmania infantum is one of the causative agents of visceral leishmaniasis (VL). VL is the most severe form of leishmaniasis and can be fatal if it is not properly treated. Although several PCR works are intended to detect L. infantum, in silico analysis of available primers and/or primer-probes reveals potential cross species amplification. Here, a TaqMan-based quantitative real time PCR (qPCR) assay was developed for specific detection and quantitation of L. infantum in tissue samples from experimentally or naturally infected animals, mice or dogs, respectively. For this assay, primers and probes were designed for the kinetoplast minicircle DNA of L. infantum. The qPCR assay achieved a detection limit of 0.01pg of parasite DNA, and allowed specific amplification of L. infantum in both asymptomatic and symptomatic naturally infected dogs with inter-assay variation coefficients between 0.05-0.11. There was no cross amplification with dog DNA or with L. braziliensis, L. donovani, L. major, L. tropica or Trypanosoma cruzi. In addition, our assay detected a significantly higher parasite load in symptomatic than in the asymptomatic animals (p<0.0001). We believe this approach will be a valuable tool for the specific detection of L. infantum in regions of sympatric transmission of VL-causing parasites.