RESUMO
In 2000, transgenes were detected in local maize varieties (landraces) in the mountains of Oaxaca, Mexico [Quist, D. & Chapela, I. H. (2001) Nature 414, 541-543]. This region is part of the Mesoamerican center of origin for maize (Zea mays L.), and the genetic diversity that is maintained in open-pollinated landraces is recognized as an important genetic resource of great cultural value. The presence of transgenes in landraces was significant because transgenic maize has never been approved for cultivation in Mexico. Here we provide a systematic survey of the frequency of transgenes in currently grown landraces. We sampled maize seeds from 870 plants in 125 fields and 18 localities in the state of Oaxaca during 2003 and 2004. We then screened 153,746 sampled seeds for the presence of two transgene elements from the 35S promoter of the cauliflower mosaic virus and the nopaline synthase gene (nopaline synthase terminator) from Agrobacterium tumefaciens. One or both of these transgene elements are present in all transgenic commercial varieties of maize. No transgenic sequences were detected with highly sensitive PCR-based markers, appropriate positive and negative controls, and duplicate samples for DNA extraction. We conclude that transgenic maize seeds were absent or extremely rare in the sampled fields. This study provides a much-needed preliminary baseline for understanding the biological, socioeconomic, and ethical implications of the inadvertent dispersal of transgenes from the United States and elsewhere to local landraces of maize in Mexico.
Assuntos
Plantas Geneticamente Modificadas/genética , Zea mays/genética , Agrobacterium tumefaciens/genética , Aminoácido Oxirredutases/genética , Caulimovirus/genética , DNA Recombinante/genética , DNA Recombinante/isolamento & purificação , Alimentos Geneticamente Modificados/efeitos adversos , Engenharia Genética/efeitos adversos , México , Plantas Geneticamente Modificadas/efeitos adversos , Regiões Promotoras Genéticas , Sementes/genéticaAssuntos
Anticoncepcionais Pós-Coito , Estrogênios , Progestinas , Animais , Estrogênios/administração & dosagem , Etinilestradiol/administração & dosagem , Feminino , Antagonistas de Hormônios/administração & dosagem , Humanos , Levanogestrel/administração & dosagem , Mifepristona/administração & dosagem , Gravidez , Progestinas/administração & dosagemRESUMO
In a study of sexual reproduction in long-lived semelparous plants, we observed Agave macroacantha in the tropical desert of Tehuacán-Cuicatlán, Mexico, describing duration of flowering, flower phenology, and nectar production patterns. We also performed two manipulative experiments evaluating (a) the seed production efficiency of different crossing systems (selfing, cross-pollination, apomixis, and control), and (b) the effect of different pollinators (diurnal exposure to pollinators, nocturnal exposure, exclusion, and control) on the seeds produced. Flowering occurred from early May to late July and had a mean duration of 29 days in the individual rosettes. The flowers were protandrous; anthesis occurred in the afternoon of the third day after floral opening, and the pistils matured in the afternoon of the fifth day. The stigmas remained receptive from dusk to the following morning. Pollination was mostly allogamous. Nectar was produced principally during the night, from the first stages of floral aperture until the stigmas wilted and flowering ceased. The flowers were visited during the day by hymenoptera, butterflies, and hummingbirds and during the night by bats and moths. Only the nocturnal visitors, however, were successful pollinators. Agave macroacantha is extremely dependent on nocturnal pollinators for its reproductive success.
RESUMO
We did a series of observational studies and manipulative experiments on the guild of nocturnal visitors of Agave macroacantha, including (1) a description of the hourly patterns of visits by moths and bats, (2) an evaluation of the relative contribution of bats and moths to flowering success, and (3) an evaluation of the pollination efficiency of the different bat species. Scapes exposed to moths but excluded to bats yielded â¼50% fewer fruits than those exposed to both pollinator groups. Flowers exposed to the bat species Leptonycteris curasoae showed similar fruiting success to those exposed to Choeronycteris mexicana and to those exposed to the whole nocturnal visitor guild. However, the fruits originated from flowers pollinated by Leptonycteris curasoae yielded significantly more seed than those exposed to Choeronycteris mexicana or to the whole pollinator guild. It is concluded that Agave macroacantha is extremely dependent on nocturnal pollinators for its reproductive success and that bats are especially important for successful pollination. Some of these pollinators are migratory and have been reported to be steadily declining. A continuing decline in the populations of pollinators may impede the successful sexual reproduction of the plant host and may put the long-term survival of this agave species under risk.
RESUMO
Two field experiments were conducted using three dominant perennial species of the Chihuahuan Desert: Hilaria mutica (a tussock grass), Larrea tridentata (a microphyllous shrub) and Opuntia rastrera (a flat-stemmed succulent cactus). Two hypotheses concerning competition in arid plant communities were tested. (1) Marked resource partitioning with no interspecific competition could be expected since the three species belong to different life-forms, and that plant growth in deserts is basically limited by harsh environmental conditions. (2) Alternatively, resource scarcity (particularly water) will result in strong plant competition. In a 1-year removal experiment, water status and plant growth of the three species were monitored in twelve 10 m × 10 m plots randomized in three blocks and assigned to the following treatments: (a) removal of all species, except H. mutica; (b) removal of all species, except L. tridentata; (c) removal of all species, except O. rastrera, and (d) control without any manipulation. In a watering experiment, under two neighbourhood conditions (growing isolated or in associations of plants of the three species in plots of 20 m2), the water status of the three species and the growth of H.mutica and L.tridentata were studied for 32 days after an irrigation equivalent to 30 mm of rain, similar to a strong storm event at the site. In the removal experiment, where plants were free to capture water, no evidence of competition was observed. However, during the watering experiment, in which water was forced into the soil, competitive effects were observed. Associated individuals of L. tridentata had lower xylem water potentials and osmotic potentials (OPs) and produced shorter twigs and less leaves and nodes. Although less pronounced, neighbours also had a negative effect on the OP in O. rastrera. According to these results, the intensity of the interspecific competition for water seems to depend on the level of resource availability in the soil. Thus, the validity of the two hypotheses tested in this study also depends on the level of resources. Competition could be absent or very low in years of low precipitation, as in the year of this study (173 mm against a 25-year average of 264 mm). However, when soil water availability is high, e.g. following heavy rain, the negative interactions between species could be more intense.
RESUMO
PIP: This article describes the poor environmental and living conditions in Mexico City due to its huge size. Mexico City's size is a challenge to sustainability, and the outcome is unknown. Mexico City and the geographic basin surrounding it included about 18.5 million population in 1995. The basin and surrounding volcanic ranges include nine major environmental zones. Urban growth followed four stages. Different cultures applied different solutions to water supply problems. The basin shifted from self-sufficiency to reliance on 31% of supplies from external watersheds. The water table is declining and canals are polluted. Irrigated agriculture is disappearing. There is an average water deficit of over 800 million cubic meters per year. Mexico City is actually sinking due to groundwater exploitation. There is bacterial contamination of wells due to improper seals. About 75% of the population has access to wastewater treatment and sanitation, but sewage treatment plants operate at under 50% efficiency and treat only about 7% of the total wastewater. Atmospheric pollution from suspended particles has been a problem for decades. Ozone was the most significant air contaminant in 1994. Lead was the most harmful pollutant in 1986. Air pollutants may be the source of submucosal inflammations. Industrial areas are contaminated with suspended particles and sulfur dioxide. High traffic areas have high carbon monoxide levels. Atmospheric pollution has affected the quality of the rainwater. The city survives by importing food, energy, wood, water, building materials, and other products. The development model aims to improve quality of life. The city has been the center of political power since Aztec times, and its preeminent position forces government action. The author concludes that there are limits to urbanization, which the city is approaching rapidly.^ieng
Assuntos
Conservação dos Recursos Naturais , Poluição Ambiental , Crescimento Demográfico , Qualidade de Vida , Saneamento , Urbanização , Abastecimento de Água , América , Demografia , Países em Desenvolvimento , Economia , Meio Ambiente , Geografia , Saúde , América Latina , México , América do Norte , População , Dinâmica Populacional , Saúde Pública , Pesquisa , Seguridade Social , População UrbanaRESUMO
In 24 healthy volunteers (seven women, 17 men; mean age 23 +/- 5 years), we studied the insulin response to a short stress period of 30 min, induced by cognitive conflict under social pressure. Insulin, growth hormone (GH), blood glucose and blood pressure (BP) determinations were performed before and after the stress period. There was a significant increase in insulin levels following the stress period (p = 0.02, paired t-test). A multiple stepwise regression analysis, with insulin difference as the dependent variable and initial GH and blood sugar levels, their increments and body mass index as predictors, showed that insulin variation was independent of any of the predictors. We discuss the influence of autonomic innervation on insulin secretion and the possible change in insulin sensitivity during stress.