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Introduction: The use of natural products such as essential oils has been suggested due to their promising pharmacological effects and economic viability. This study aimed to determine hydrogenic potential (pH), titratable acidity (TA), and ion concentrations of five solutions containing essential oils (EO), when used as a EO-containing solutions, and evaluate ion concentrations, enamel surface loss, and morphology alterations in enamel. Materials and methods: The pH, TA, calcium (Ca), potassium (K), and sodium (Na) concentrations of five EO-containing solutions were measured. Bovine enamel specimens were submitted to two daily 30-s immersions in artificial saliva, citric acid, distilled water, BaCloTea (Basil, Clove e Tea Tree), GeLaTeaPep (Geranium, Lavender, Tea Tree and Peppermint), EucaLem (Eucalyptus and Lemon), Cinnamon, or Spearmint solutions for 14 days. Ca, K, Na, and phosphorus (P) were quantified through ions chromatography, enamel surface loss was determined by profilometry, and surface morphology was qualitatively analyzed through scanning electron microscopy. Data were submitted to one-way ANOVA and Tukey (p < 0.05). Results: The five EO-containing solutions presented significantly lower pH values than distilled water (p < 0.05). The GeLaTeaPep group presented a significantly higher TA value than BaCloTea (p < 0.05), which in turn showed a significantly higher TA value than the other solutions (p < 0.05). The distilled water presented significantly higher Ca, K, and Na concentrations than all EO-containing solutions (p < 0.05). The enamel exposed to EO-containing solutions showed lower Ca and P concentrations than artificial saliva (control) as well as significantly higher surface loss; however, the surface morphology was similar to the artificial saliva. Conclusion: EO-containing solutions have low pH, TA, and low concentrations of Ca, Na, and K. Moreover, enamel exposed to these solutions showed low Ca and P concentrations and slight surface loss without morphology alteration.

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Anthropogenic activities may have increased the concentrations of potentially toxic elements (PTEs) in fish from the southeastern Carajás Mineral Province in Brazil, which has not yet been studied. The objectives were to determine the quality parameters of surface water and bottom sediments, and to assess the bioaccumulation and risks of Al, Fe, Ba, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Ti, V and Zn in fish species from the southeastern Carajás Mineral Province. Water, sediments and fish species (Colossoma macropomum, Curimata cyprinoides, Geophagus sp., Leporinus trifasciatus, and Serrasalmus eigenmanni) were collected in 14 areas in the municipalities of Parauapebas, Marabá and Canaã dos Carajás, contemplating the Gelado Stream and the Parauapebas, Tapirapé and Itacaiúnas Rivers. Water samples were subjected to physicochemical analysis using a multiparameter meter. Concentrations of PTEs in all samples were quantified by inductively coupled plasma-optical emission spectrometry. Enrichment factor (EF) and geoaccumulation index (Igeo) were calculated to study the sediment enrichment and contamination. Ecological and human health risk assessments were performed to determine the risks to the environment and population's health. EF and Igeo revealed that the sediments from the Parauapebas River and Gelado Stream are respectively enriched by Ba, Co, Cu, Cr, Mn, Pb, and Ba, Co, Cr, Ni, Pb and V. The concentrations of Fe (1.67 mg L-1) and Mn (0.11 mg L-1) in water and the concentrations of Cr (>0.1 mg kg-1) and Pb (>2 mg kg-1) in fish were above the Brazilian legislation thresholds. The ecological risk assessment revealed considerable risk from Ni and moderate risk from multiple PTEs in sediments from the Gelado Stream. Human health risks were detected for Pb in all fish species and for Mo in L.trifasciatus. These results indicate that techniques for monitoring and controlling contamination must be implemented by the environmental agencies.

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This work presents a new procedure to synthesize ruthenium-phthalocyanine complexes and uses diverse spectroscopic techniques to characterize trans-[RuCl(Pc)DMSO] (I) (Pc = phthalocyanine) and trans-[Ru(Pc)(4-ampy)2] (II) (4-ampy = 4-aminopyridine). The triplet excited-state lifetimes of (I) measured by nanosecond transient absorption showed that two processes occurred, one around 15 ns and the other around 3.8 µs. Axial ligands seemed to affect the singlet oxygen quantum yield. Yields of 0.62 and 0.14 were achieved for (I) and (II), respectively. The lower value obtained for (II) probably resulted from secondary reactions of singlet oxygen in the presence of the ruthenium complex. We also investigate how axial ligands in the ruthenium-phthalocyanine complexes affect their photo-bioactivity in B16F10 murine melanoma cells. In the case of (I) at 1 µmol/L, photosensitization with 5.95 J/cm2 provided B16F10 cell viability of 6%, showing that (I) was more active than (II) at the same concentration. Furthermore, (II) was detected intracellularly in B16F10 cell extracts. The behavior of the evaluated ruthenium-phthalocyanine complexes point to the potential use of (I) as a metal-based drug in clinical therapy. Changes in axial ligands can modulate the photosensitizer activity of the ruthenium phthalocyanine complexes.

RESUMO

The present study reports two outbreaks of salt poisoning in goats on a property in the state of Pará, Brazil. The outbreaks occurred in July and August 2011 when young and adult goats received a mineral mixture after approximately three days of restriction from the supplement. The animals were kept in barns with a high stocking density and had an inadequate supply of water. In the second outbreak, the goats were supplemented too with brewery residue added to 3.3% of the mixture mineral. From a flock of 191 goats, 12 animals (nine crossbreeds, two Anglo-Nubians and one Boer) showed nervous symptomatology, and the morbidity rate was 6.28%. The most frequent clinical signs were head lowering, head tilt, muscle weakness, mydriasis and ataxia. Three goats died, four recovered spontaneously from the first outbreak, and five recovered from the second outbreak over three days after treatment with dexamethasone, thiamine and gradual water supply. The lethality rate was 25%. In two animals, necropsy was performed, and no macro- or microscopic alterations in the nervous system or other organs were observed. These negative pathological findings indicate that the neurological clinical signs were initiated by functional disturbances in the central nervous system. In the brain, a high sodium concentration (3703 and 3675 ppm), compared with the reference values for cattle was detected...(AU)

Este trabalho relata dois surtos de intoxicação por sal em caprinos em uma propriedade no estado do Pará, Brasil. Os surtos ocorreram em julho e agosto de 2011 quando caprinos jovens e adultos receberam mistura mineral, após cerca de três dias de restrição do suplemento. Os animais estavam estabulados em baias com lotação acima do recomendado e os bebedouros não atendiam um adequado fornecimento de água. No segundo surto, os caprinos foram suplementados adicionalmente com resíduos de cervejaria acrescido de 3,3% de mistura mineral. De um total de 191 caprinos, nove mestiços, dois Anglonubianos e um Boer, apresentaram sintomatologia nervosa observando-se taxa de morbidade de 6,28%. Os sinais clínicos mais frequentes foram fraqueza muscular, relutância ao movimento, rotação lateral da cabeça com o pescoço estendido e cabeça baixa. Três caprinos morreram, quatro do primeiro surto recuperaram-se espontaneamente, e cinco recuperarm-se após três dias de tratamento com dexametasona, tiamina e fornecimento gradual de água. A taxa de letalidade foi de 25%. Em dois animais necropsiados, não foram observadas alterações macro e microscópicas no sistema nervoso e em outros órgãos. Estes resultados negativos apontaram que os sinais clínicos neurológicos foram desencadeados por distúrbios funcionais no sistema nervoso central. No cérebro destes dois, havia altas concentrações de sódio...(AU)

Assuntos

RESUMO

The present study reports two outbreaks of salt poisoning in goats on a property in the state of Pará, Brazil. The outbreaks occurred in July and August 2011 when young and adult goats received a mineral mixture after approximately three days of restriction from the supplement. The animals were kept in barns with a high stocking density and had an inadequate supply of water. In the second outbreak, the goats were supplemented too with brewery residue added to 3.3% of the mixture mineral. From a flock of 191 goats, 12 animals (nine crossbreeds, two Anglo-Nubians and one Boer) showed nervous symptomatology, and the morbidity rate was 6.28%. The most frequent clinical signs were head lowering, head tilt, muscle weakness, mydriasis and ataxia. Three goats died, four recovered spontaneously from the first outbreak, and five recovered from the second outbreak over three days after treatment with dexamethasone, thiamine and gradual water supply. The lethality rate was 25%. In two animals, necropsy was performed, and no macro- or microscopic alterations in the nervous system or other organs were observed. These negative pathological findings indicate that the neurological clinical signs were initiated by functional disturbances in the central nervous system. In the brain, a high sodium concentration (3703 and 3675 ppm), compared with the reference values for cattle was detected...

Este trabalho relata dois surtos de intoxicação por sal em caprinos em uma propriedade no estado do Pará, Brasil. Os surtos ocorreram em julho e agosto de 2011 quando caprinos jovens e adultos receberam mistura mineral, após cerca de três dias de restrição do suplemento. Os animais estavam estabulados em baias com lotação acima do recomendado e os bebedouros não atendiam um adequado fornecimento de água. No segundo surto, os caprinos foram suplementados adicionalmente com resíduos de cervejaria acrescido de 3,3% de mistura mineral. De um total de 191 caprinos, nove mestiços, dois Anglonubianos e um Boer, apresentaram sintomatologia nervosa observando-se taxa de morbidade de 6,28%. Os sinais clínicos mais frequentes foram fraqueza muscular, relutância ao movimento, rotação lateral da cabeça com o pescoço estendido e cabeça baixa. Três caprinos morreram, quatro do primeiro surto recuperaram-se espontaneamente, e cinco recuperarm-se após três dias de tratamento com dexametasona, tiamina e fornecimento gradual de água. A taxa de letalidade foi de 25%. Em dois animais necropsiados, não foram observadas alterações macro e microscópicas no sistema nervoso e em outros órgãos. Estes resultados negativos apontaram que os sinais clínicos neurológicos foram desencadeados por distúrbios funcionais no sistema nervoso central. No cérebro destes dois, havia altas concentrações de sódio...

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Piper arboreum Aubl. var. arboreum and P. tuberculatum Jacq. (Piperaceae), both known as "pimenta-longa", are frequently misidentified. Despite the fact that both species are sold as traditional phytotherapeutic products, there are no reports on their quality control. The aim of this study was to prepare and characterize leaf and stem herbal drugs of both species using standard methods aiming to establish parameters for botanical authentication and purity degree for their quality control. Size, shape, color, taste, texture, fracture surface and transection characteristics, and leaf venation patterns are macroscopic diagnostic characters useful to distinguish the herbal drugs when they are not powdered. The following characters are useful for diagnostic purposes of the powdered herbal drugs: cuticular and epicuticular wax patterns, types of trichomes, and wall thickening of the tracheary elements, and shape of the hilum of the starch grains. Emergences are key morphological markers for P. tuberculatum herbal drugs. Physicochemical parameter values were similar in herbal drugs of both species, except acid-insoluble ash value which was higher in the P. arboreum var. arboreum stem herbal drugs. Piper arboreum var. arboreum herbal drugs had the highest metal concentrations. Al, Cu, Mn and V concentrations were the highest, whereas Cd, Co and Hg concentrations were the lowest in the herbal drugs of both species. The results obtained can be used as quality control parameters for the herbal drugs of both species.(AU)

Piper arboreum Aubl. var. arboreum e P. tuberculatum Jacq. (Piperaceae), conhecidas como pimenta-longa, são comercializadas como produtos tradicionais fitoterápicos e, frequentemente, identificadas erroneamente, não havendo relatos sobre controle de qualidade dessas espécies. O objetivo do trabalho foi preparar e caracterizar drogas vegetais foliares e caulinares dessas espécies para estabelecer-lhes parâmetros de autenticidade botânica e grau de pureza para o controle de qualidade. Foram utilizados métodos padrões para o preparo e análises estrutural e físico-química das drogas vegetais. São caracteres diagnósticos macroscópicos para distinguir as drogas vegetais íntegras: tamanho, forma, cor, sabor, textura, características da superfície de fratura e da secção transversal e os padrões de venação foliar. São caracteres diagnósticos para distinguir as drogas vegetais pulverizadas: padrões de cutícula e cera epicuticular, tipos de tricoma e espessamento parietal dos elementos traqueais e a forma do hilo dos grãos de amido. Emergências são marcadores morfológicos chave para as drogas vegetais de P. tuberculatum. Os valores dos parâmetros físico-químicos foram similares nas drogas vegetais das espécies, exceto o de cinzas ácido-insolúveis que foi maior nas drogas vegetais caulinares de P. arboreum var. arboreum. As maiores concentrações de metais ocorreram nas drogas vegetais de P. arboreum var. arboreum. As concentrações mais elevadas foram de Al, Cu, Mn e V e as mais baixas de Cd, Co e Hg. Os resultados obtidos poderão ser utilizados como parâmetros de controle de qualidade para as drogas vegetais de ambas as espécies.(AU)

Assuntos

RESUMO

Piper arboreum Aubl. var. arboreum and P. tuberculatum Jacq. (Piperaceae), both known as "pimenta-longa", are frequently misidentified. Despite the fact that both species are sold as traditional phytotherapeutic products, there are no reports on their quality control. The aim of this study was to prepare and characterize leaf and stem herbal drugs of both species using standard methods aiming to establish parameters for botanical authentication and purity degree for their quality control. Size, shape, color, taste, texture, fracture surface and transection characteristics, and leaf venation patterns are macroscopic diagnostic characters useful to distinguish the herbal drugs when they are not powdered. The following characters are useful for diagnostic purposes of the powdered herbal drugs: cuticular and epicuticular wax patterns, types of trichomes, and wall thickening of the tracheary elements, and shape of the hilum of the starch grains. Emergences are key morphological markers for P. tuberculatum herbal drugs. Physicochemical parameter values were similar in herbal drugs of both species, except acid-insoluble ash value which was higher in the P. arboreum var. arboreum stem herbal drugs. Piper arboreum var. arboreum herbal drugs had the highest metal concentrations. Al, Cu, Mn and V concentrations were the highest, whereas Cd, Co and Hg concentrations were the lowest in the herbal drugs of both species. The results obtained can be used as quality control parameters for the herbal drugs of both species.

Piper arboreum Aubl. var. arboreum e P. tuberculatum Jacq. (Piperaceae), conhecidas como pimenta-longa, são comercializadas como produtos tradicionais fitoterápicos e, frequentemente, identificadas erroneamente, não havendo relatos sobre controle de qualidade dessas espécies. O objetivo do trabalho foi preparar e caracterizar drogas vegetais foliares e caulinares dessas espécies para estabelecer-lhes parâmetros de autenticidade botânica e grau de pureza para o controle de qualidade. Foram utilizados métodos padrões para o preparo e análises estrutural e físico-química das drogas vegetais. São caracteres diagnósticos macroscópicos para distinguir as drogas vegetais íntegras: tamanho, forma, cor, sabor, textura, características da superfície de fratura e da secção transversal e os padrões de venação foliar. São caracteres diagnósticos para distinguir as drogas vegetais pulverizadas: padrões de cutícula e cera epicuticular, tipos de tricoma e espessamento parietal dos elementos traqueais e a forma do hilo dos grãos de amido. Emergências são marcadores morfológicos chave para as drogas vegetais de P. tuberculatum. Os valores dos parâmetros físico-químicos foram similares nas drogas vegetais das espécies, exceto o de cinzas ácido-insolúveis que foi maior nas drogas vegetais caulinares de P. arboreum var. arboreum. As maiores concentrações de metais ocorreram nas drogas vegetais de P. arboreum var. arboreum. As concentrações mais elevadas foram de Al, Cu, Mn e V e as mais baixas de Cd, Co e Hg. Os resultados obtidos poderão ser utilizados como parâmetros de controle de qualidade para as drogas vegetais de ambas as espécies.

Assuntos