RESUMO
Infections caused by uncommon and resistant pathogens in unusual sites have been increasingly reported in medical literature. We describe four cases of rare cytological findings and clinical impact for patients. In the first case, Aspergillus sp and Pneumocystis jirovecii were observed in the bronchoalveolar lavage of a patient with severe systemic lupus. In the second and third cases, we describe the presence of Trichomonas sp and Strongyloides sp larvae in samples of pleural and peritoneal fluid, respectively. The fourth report is about a patient with a wrist subcutaneous nodule whose synovial aspiration and cytology revealed the presence of brown septate hyphae. The early identification of the infectious agent in the cytological examination was essential for the introduction and/or re-adaptation of therapy in the four cases described. Patients in this report were immunocompromised with severe comorbidities, conditions often associated with unfavorable clinical outcomes.
Assuntos
Doenças Transmissíveis/diagnóstico , Citodiagnóstico/métodos , Animais , Líquido Ascítico/parasitologia , Aspergillus/isolamento & purificação , Líquido da Lavagem Broncoalveolar/microbiologia , Evolução Fatal , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Derrame Pleural/parasitologia , Pneumocystis carinii/isolamento & purificação , Strongyloides/isolamento & purificação , Estrongiloidíase/diagnóstico , Trichomonas/isolamento & purificação , Tricomoníase/diagnósticoRESUMO
Infections caused by uncommon and resistant pathogens in unusual sites have been increasingly reported in medical literature. We describe four cases of rare cytological findings and clinical impact for patients. In the first case, Aspergillus sp and Pneumocystis jirovecii were observed in the bronchoalveolar lavage of a patient with severe systemic lupus. In the second and third cases, we describe the presence of Trichomonas sp and Strongyloides sp larvae in samples of pleural and peritoneal fluid, respectively. The fourth report is about a patient with a wrist subcutaneous nodule whose synovial aspiration and cytology revealed the presence of brown septate hyphae. The early identification of the infectious agent in the cytological examination was essential for the introduction and/or re-adaptation of therapy in the four cases described. Patients in this report were immunocompromised with severe comorbidities, conditions often associated with unfavorable clinical outcomes.
Assuntos
Humanos , Animais , Masculino , Feminino , Pessoa de Meia-Idade , Doenças Transmissíveis/diagnóstico , Citodiagnóstico/métodos , Derrame Pleural/parasitologia , Aspergillus/isolamento & purificação , Strongyloides/isolamento & purificação , Estrongiloidíase/diagnóstico , Trichomonas/isolamento & purificação , Tricomoníase/diagnóstico , Líquido Ascítico/parasitologia , Líquido da Lavagem Broncoalveolar/microbiologia , Evolução Fatal , Pneumocystis carinii/isolamento & purificaçãoRESUMO
Objetivou-se estimar a associação fenotípica entre a eficiência alimentar e o ganho em acabamento de carcaça de touros jovens da raça Nelore. Utilizaram-se informações de 98 touros jovens da raça Nelore, participantes de duas provas de eficiência alimentar que empregaram o sistema de cochos eletrônicos Growsafe®, as quais foram realizadas nos anos de 2015 e 2016, na fazenda Capim Branco, da Universidade Federal de Uberlândia. As provas de eficiência alimentar tiveram duração de 91 dias, sendo 21 dias de adaptação e 70 dias de avaliação. Os animais apresentavam idade média ao início e ao término do confinamento de 19,98 e 22,16 meses e peso inicial e final médio de 428,17 e 542,27kg, respectivamente. Verificou-se que os animais mais eficientes depositaram menos gordura, e os animais menos eficientes depositaram mais gordura durante o período de avaliação em confinamento. Observou-se que há uma associação fenotípica de baixa magnitude (0,29) entre o consumo alimentar residual (CAR) e o ganho em acabamento de carcaça (GACAB). Assim, pode-se inferir que, ao serem selecionados menores valores de CAR, haverá alguma interferência negativa em GACAB. Concluiu-se que animais mais eficientes (CAR negativo) podem apresentar menor deposição de gordura subcutânea, influenciando na qualidade de carcaça de bovinos da raça Nelore.(AU)
This study aimed to estimate the phenotypic association between feed efficiency and gain in carcass finishing of young Nelore bulls. Information from 98 young Nelore bulls participating in two feed efficiency tests using the Growsafe® electronic trough system, carried out in the years 2015 and 2016, at the Capim Branco farm of the Universidade Federal de Uberlândia was used .The feed efficiency tests lasted for 91 days, with 21 days of adaptation and 70 days of evaluation. The animals had a mean age at the beginning and end of the confinement of 19.98 and 22.16 months, and mean weight initial and end of 428.17 and 542.27kg, respectively. It was verified that most efficient animals deposited less fat, and the efficient animals deposited more fat during the evaluation period at the confinement. It was observed that there is a low magnitude phenotypic association (0.29) between the residual feed intake (RFI) and the gain in carcass finishing (GACAB). Thus, it can be inferred that by selecting lower values of residual feed intake there will be some negative interference in GACAB. It was concluded that the most efficient animals (negative RFI) may present lower deposition of subcutaneous fat, influencing the carcass quality of Nelore cattle.(AU)
Assuntos
Animais , Bovinos , Aumento de Peso , Ingestão de Alimentos , Gordura SubcutâneaRESUMO
Objetivou-se estimar a associação fenotípica entre a eficiência alimentar e o ganho em acabamento de carcaça de touros jovens da raça Nelore. Utilizaram-se informações de 98 touros jovens da raça Nelore, participantes de duas provas de eficiência alimentar que empregaram o sistema de cochos eletrônicos Growsafe®, as quais foram realizadas nos anos de 2015 e 2016, na fazenda Capim Branco, da Universidade Federal de Uberlândia. As provas de eficiência alimentar tiveram duração de 91 dias, sendo 21 dias de adaptação e 70 dias de avaliação. Os animais apresentavam idade média ao início e ao término do confinamento de 19,98 e 22,16 meses e peso inicial e final médio de 428,17 e 542,27kg, respectivamente. Verificou-se que os animais mais eficientes depositaram menos gordura, e os animais menos eficientes depositaram mais gordura durante o período de avaliação em confinamento. Observou-se que há uma associação fenotípica de baixa magnitude (0,29) entre o consumo alimentar residual (CAR) e o ganho em acabamento de carcaça (GACAB). Assim, pode-se inferir que, ao serem selecionados menores valores de CAR, haverá alguma interferência negativa em GACAB. Concluiu-se que animais mais eficientes (CAR negativo) podem apresentar menor deposição de gordura subcutânea, influenciando na qualidade de carcaça de bovinos da raça Nelore.(AU)
This study aimed to estimate the phenotypic association between feed efficiency and gain in carcass finishing of young Nelore bulls. Information from 98 young Nelore bulls participating in two feed efficiency tests using the Growsafe® electronic trough system, carried out in the years 2015 and 2016, at the Capim Branco farm of the Universidade Federal de Uberlândia was used .The feed efficiency tests lasted for 91 days, with 21 days of adaptation and 70 days of evaluation. The animals had a mean age at the beginning and end of the confinement of 19.98 and 22.16 months, and mean weight initial and end of 428.17 and 542.27kg, respectively. It was verified that most efficient animals deposited less fat, and the efficient animals deposited more fat during the evaluation period at the confinement. It was observed that there is a low magnitude phenotypic association (0.29) between the residual feed intake (RFI) and the gain in carcass finishing (GACAB). Thus, it can be inferred that by selecting lower values of residual feed intake there will be some negative interference in GACAB. It was concluded that the most efficient animals (negative RFI) may present lower deposition of subcutaneous fat, influencing the carcass quality of Nelore cattle.(AU)
Assuntos
Animais , Bovinos , Aumento de Peso , Gordura SubcutâneaRESUMO
The objective of this study was to evaluate the health status, productive performance and carcass traits of growing rabbits housed in pen with litter and conventional cage. At 35 d, 54 crossbred rabbits (New Zealand White vs. Botucatu) were randomly housed in pen with litter (6 pens, 6 rabbits/pen, 2.3 rabbits/m²) or cage (6 cages, 3 rabbits/cage, 8.3 rabbits/m²) and kept until the age of slaughter at 77 d. To simulate farm conditions, the rabbits that were housed in pen have not been previously adapted to this system. There was greater intensity of dirt and mucus occurrence in the rabbits housed in pens in first period, but the mucus occurrence practically disappeared in the later period. For performance, the rabbits housed in cages had a higher live weight at 56 d (1714 vs. 1506 g; P0.05) in the posterior period (56 to 77 d). There was no effect (P>0.05) of housing system for carcass yield, dissectible fat and hind leg yield. It may be suggested that rabbits adapt to the floor over time. Therefore, it is indicated the adoption of this system for growing rabbits.
O objetivo deste estudo foi avaliar o estado sanitário, o desempenho produtivo e as características de carcaça de coelhos em crescimento alojados em piso com cama e em gaiola convencional Aos 35 dias, 54 coelhos mestiços (Nova Zelândia Branco x Botucatu) foram alojados aleatoriamente em piso com cama (6 boxes; 6 coelhos/box; 2,3 coelhos/m²) ou em gaiola (6 gaiolas; 3 coelhos/gaiola; 8,3 coelhos/m²) e mantidos até a idade de abate, aos 77 dias. Para simular as condições de granja, os coelhos alojados em piso não foram previamente adaptados a este sistema. Houve maior intensidade de sujeira e ocorrência de muco nos coelhos alojados em piso no primeiro período, entretanto, esta ocorrência praticamente desapareceu no período posterior. Para o desempenho, os coelhos alojados em gaiola apresentaram maior peso vivo aos 56 dias (1714 vs. 1506 g; P0,05) os resultados de melhor desempenho no período posterior (56 a 77 dias). Não houve efeito (P>0,05) de sistema de alojamento para os rendimentos de carcaça referência, gordura dissecável e de pernil. Pode-se sugerir a ocorrência de adaptação dos coelhos ao piso, ao longo do tempo. Portanto, é indicada a adoção do sistema de alojamento em piso com cama para coelhos em crescimento.
Assuntos
Animais , Coelhos , Alojamento , Animais Domésticos/crescimento & desenvolvimento , Vigilância SanitáriaRESUMO
The objective of this study was to evaluate the health status, productive performance and carcass traits of growing rabbits housed in pen with litter and conventional cage. At 35 d, 54 crossbred rabbits (New Zealand White vs. Botucatu) were randomly housed in pen with litter (6 pens, 6 rabbits/pen, 2.3 rabbits/m²) or cage (6 cages, 3 rabbits/cage, 8.3 rabbits/m²) and kept until the age of slaughter at 77 d. To simulate farm conditions, the rabbits that were housed in pen have not been previously adapted to this system. There was greater intensity of dirt and mucus occurrence in the rabbits housed in pens in first period, but the mucus occurrence practically disappeared in the later period. For performance, the rabbits housed in cages had a higher live weight at 56 d (1714 vs. 1506 g; P<0.001), as well as BWG (46.4 vs. 36.7 g/d, P<0.001) and better FC (2.75 vs. 3.33; P<0.05) from 35 to 56 d. However, these animals did not show the best performance (P>0.05) in the posterior period (56 to 77 d). There was no effect (P>0.05) of housing system for carcass yield, dissectible fat and hind leg yield. It may be suggested that rabbits adapt to the floor over time. Therefore, it is indicated the adoption of this system for growing rabbits.(AU)
O objetivo deste estudo foi avaliar o estado sanitário, o desempenho produtivo e as características de carcaça de coelhos em crescimento alojados em piso com cama e em gaiola convencional Aos 35 dias, 54 coelhos mestiços (Nova Zelândia Branco x Botucatu) foram alojados aleatoriamente em piso com cama (6 boxes; 6 coelhos/box; 2,3 coelhos/m²) ou em gaiola (6 gaiolas; 3 coelhos/gaiola; 8,3 coelhos/m²) e mantidos até a idade de abate, aos 77 dias. Para simular as condições de granja, os coelhos alojados em piso não foram previamente adaptados a este sistema. Houve maior intensidade de sujeira e ocorrência de muco nos coelhos alojados em piso no primeiro período, entretanto, esta ocorrência praticamente desapareceu no período posterior. Para o desempenho, os coelhos alojados em gaiola apresentaram maior peso vivo aos 56 dias (1714 vs. 1506 g; P<0,001), bem como, maior ganho de peso diário (46,4 vs. 36,7 g/d; P<0,001) e melhor conversão alimentar (2,75 vs. 3,33; P<0,05), no período de 35 a 56 dias. Entretanto, estes animais não mantiveram (P>0,05) os resultados de melhor desempenho no período posterior (56 a 77 dias). Não houve efeito (P>0,05) de sistema de alojamento para os rendimentos de carcaça referência, gordura dissecável e de pernil. Pode-se sugerir a ocorrência de adaptação dos coelhos ao piso, ao longo do tempo. Portanto, é indicada a adoção do sistema de alojamento em piso com cama para coelhos em crescimento.(AU)
Assuntos
Animais , Coelhos , Animais Domésticos/crescimento & desenvolvimento , Alojamento , Vigilância SanitáriaRESUMO
The objective of this study was to evaluate the health status, productive performance and carcass traits of growing rabbits housed in pen with litter and conventional cage. At 35 d, 54 crossbred rabbits (New Zealand White vs. Botucatu) were randomly housed in pen with litter (6 pens, 6 rabbits/pen, 2.3 rabbits/m²) or cage (6 cages, 3 rabbits/cage, 8.3 rabbits/m²) and kept until the age of slaughter at 77 d. To simulate farm conditions, the rabbits that were housed in pen have not been previously adapted to this system. There was greater intensity of dirt and mucus occurrence in the rabbits housed in pens in first period, but the mucus occurrence practically disappeared in the later period. For performance, the rabbits housed in cages had a higher live weight at 56 d (1714 vs. 1506 g; P
RESUMO
Cofilin-1 (CFL1), a small protein of 18 kDa, has been studied as a biomarker due to its involvement in tumor cell migration and invasion. Our aim was to evaluate CFL1 as an indicator of malignancy and aggressiveness in sputum samples. CFL1 was analyzed by ELISA immunoassay in the sputum of 73 lung cancer patients, 13 cancer-free patients, and 6 healthy volunteers. Statistical analyses included ANOVA, ROC curves, Spearman correlation, and logistic regression. Sputum CFL1 levels were increased in cancer patients compared to cancer-free patients and volunteers (P<0.05). High expression of sputum CFL1 was correlated to T4 stage (P=0.01) and N stage (P=0.03), tobacco history (P=0.01), and squamous cell carcinoma histologic type (P=0.04). The accuracy of sputum CFL1 in discriminating cancer patients from cancer-free patients and healthy volunteers were 0.78 and 0.69, respectively. CFL1 at a cut-off value of 415.25 pg/mL showed sensitivity/specificity of 0.80/0.70 in differentiating between healthy volunteers and cancer patients. Sputum CFL1 was also able to identify cancer-free patients from patients with lung cancer. The AUC was 0.70 and, at a cut-off point ≥662.63 pg/mL, we obtained 60% sensitivity and 54% specificity. Logistic regression analysis controlled for tobacco history, histologic types, and N stage showed that cancer cell-associated CFL1 was an independent predictor of death. Smoker patients with squamous cell carcinoma, lymph node metastasis and sputum CFL1>1.475 pg/mL showed augmented chance of death, suggesting lung cancer aggressiveness. CFL1 presented diagnostic value in detecting lung cancer and was associated to tumor aggressiveness.
Assuntos
Biomarcadores Tumorais/análise , Carcinoma de Células Escamosas/química , Cofilina 1/análise , Neoplasias Pulmonares/química , Escarro/química , Adulto , Idoso , Carcinoma de Células Escamosas/mortalidade , Carcinoma de Células Escamosas/patologia , Estudos de Casos e Controles , Proliferação de Células , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Estadiamento de Neoplasias , Prognóstico , Curva ROC , Sensibilidade e EspecificidadeRESUMO
Cofilin-1 (CFL1), a small protein of 18 kDa, has been studied as a biomarker due to its involvement in tumor cell migration and invasion. Our aim was to evaluate CFL1 as an indicator of malignancy and aggressiveness in sputum samples. CFL1 was analyzed by ELISA immunoassay in the sputum of 73 lung cancer patients, 13 cancer-free patients, and 6 healthy volunteers. Statistical analyses included ANOVA, ROC curves, Spearman correlation, and logistic regression. Sputum CFL1 levels were increased in cancer patients compared to cancer-free patients and volunteers (P<0.05). High expression of sputum CFL1 was correlated to T4 stage (P=0.01) and N stage (P=0.03), tobacco history (P=0.01), and squamous cell carcinoma histologic type (P=0.04). The accuracy of sputum CFL1 in discriminating cancer patients from cancer-free patients and healthy volunteers were 0.78 and 0.69, respectively. CFL1 at a cut-off value of 415.25 pg/mL showed sensitivity/specificity of 0.80/0.70 in differentiating between healthy volunteers and cancer patients. Sputum CFL1 was also able to identify cancer-free patients from patients with lung cancer. The AUC was 0.70 and, at a cut-off point ≥662.63 pg/mL, we obtained 60% sensitivity and 54% specificity. Logistic regression analysis controlled for tobacco history, histologic types, and N stage showed that cancer cell-associated CFL1 was an independent predictor of death. Smoker patients with squamous cell carcinoma, lymph node metastasis and sputum CFL1>1.475 pg/mL showed augmented chance of death, suggesting lung cancer aggressiveness. CFL1 presented diagnostic value in detecting lung cancer and was associated to tumor aggressiveness.
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Escarro/química , Carcinoma de Células Escamosas/química , Biomarcadores Tumorais/análise , Cofilina 1/análise , Neoplasias Pulmonares/química , Prognóstico , Ensaio de Imunoadsorção Enzimática , Carcinoma de Células Escamosas/mortalidade , Carcinoma de Células Escamosas/patologia , Estudos de Casos e Controles , Curva ROC , Sensibilidade e Especificidade , Proliferação de Células , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Invasividade Neoplásica , Estadiamento de NeoplasiasRESUMO
Objetivou-se avaliar a influência da qualidade da informação na predição de valores genéticos para características de crescimento em bovinos da raça Nelore. Foram utilizadas informações de fazendas participantes do Programa Nelore Brasil da Associação Nacional de Criadores e Pesquisadores, considerando-se dados zootécnicos obtidos entre 2012 e 2013. Foram analisadas as características de crescimento relacionadas aos pesos aos 120 (P120), 210 (P210), 365 (P365) e 450 (P450) dias de idade, sob diferentes cenários: inclusão de todas as informações de lote de manejo (cenário de referência) e inclusão aleatória de 90%, 70%, 50%, 30% e 0% das informações de lote de manejo dos animais com medidas fenotípicas. Os valores genéticos foram preditos mediante análises unicaracterísticas sob modelo animal. Ocorreram alterações em todos os parâmetros genéticos quando comparados aos obtidos pelo cenário de referência. Houve um aumento nas estimativas de herdabilidade à medida que se reduziu o número de informações sobre os lotes de manejo. Esses resultados sugerem que o progresso genético estimado para rebanhos com baixa qualidade da informação zootécnica pode não expressar adequadamente o que realmente está ocorrendo com o rebanho, uma vez que as estimativas de herdabilidade podem estar infladas. Verificou-se que houve alteração na classificação dos animais para todas as características de crescimento avaliadas. A qualidade da informação zootécnica influencia na predição dos valores genéticos para as características de crescimento.(AU)
The objective was to evaluate the influence of information quality in the prediction of genetic values for the Nellore cattle's growth traits. The information came from cattle farms participating in the program of the Brazilian National Association of Breeders and Researchers (ANCP), from 2012 to 2013. Field data such as batch or management group were considered assessment criteria, and the growth traits related to body weight at 120 (W120), 210 (W210), 365 (W365) and 450 (W450) days under different scenarios: inclusion of all management lot information; and random inclusion of 90%, 70%, 50%, 30% and 0% of the batch management of information from animals with phenotypic measurements. Breeding values were predicted by using univariate models in animal model. Changes on all genetic parameters were compared to those obtained in the reference scenario. There was an increase in the estimates of heritability as it reduced the number of details about lots of management. These results suggest that gene progress estimated herds with low quality information do not adequately represent what truly occurs with the herd, since heritability estimates may be inflated. Change in the classification of animals was found at all the growth traits. The quality information influences the prediction of breeding values for growth traits.(AU)