RESUMO
The Human Secreted Group IID Phospholipase A(2) (hsPLA2GIID) may be involved in the human acute immune response. Here we have demonstrated that the hsPLA2GIID presents bactericidal and Ca(2+)-independent liposome membrane-damaging activities and we have compared these effects with the catalytic activity of active-site mutants of the protein. All mutants showed reduced hydrolytic activity against DOPC:DOPG liposome membranes, however bactericidal effects against Escherichia coli and Micrococcus luteus were less affected, with the D49K mutant retaining 30% killing of the Gram-negative bacteria at a concentration of 10µg/mL despite the absence of catalytic activity. The H48Q mutant maintained Ca(2+)-independent membrane-damaging activity whereas the G30S and D49K mutants were approximately 50% of the wild-type protein, demonstrating that phospholipid bilayer permeabilization by the hsPLA2GIID is independent of catalytic activity. We suggest that this Ca(2+)-independent damaging activity may play a role in the bactericidal function of the protein.
Assuntos
Escherichia coli/efeitos dos fármacos , Fosfolipases A2 do Grupo II/metabolismo , Fosfolipases A2 do Grupo II/farmacologia , Micrococcus luteus/efeitos dos fármacos , Antibacterianos/farmacologia , Bactérias , Cálcio/farmacologia , Membrana Celular/efeitos dos fármacos , Fosfolipases A2 do Grupo II/genética , Humanos , Hidrólise , Bicamadas Lipídicas , Lipossomos , Fosfatidilcolinas/antagonistas & inibidores , Fosfatidilgliceróis/antagonistas & inibidoresRESUMO
Suramin is a polysulphonated naphthylurea with inhibitory activity against the human secreted group IIA phospholipase A(2) (hsPLA2GIIA), and we have investigated suramin binding to recombinant hsPLA2GIIA using site-directed mutagenesis and molecular dynamics (MD) simulations. The changes in suramin binding affinity of 13 cationic residue mutants of the hsPLA2GIIA was strongly correlated with alterations in the inhibition of membrane damaging activity of the protein. Suramin binding to hsPLA2GIIA was also studied by MD simulations, which demonstrated that altered intermolecular potential energy of the suramin/mutant complexes was a reliable indicator of affinity change. Although residues in the C-terminal region play a major role in the stabilization of the hsPLA2GIIA/suramin complex, attractive and repulsive hydrophobic and electrostatic interactions with residues throughout the protein together with the adoption of a bent suramin conformation, all contribute to the stability of the complex. Analysis of the hsPLA2GIIA/suramin interactions allows the prediction of the properties of suramin analogues with improved binding and higher affinities which may be candidates for novel phospholipase A(2) inhibitors.
Assuntos
Fosfolipases A2 do Grupo II/química , Suramina/química , Sítios de Ligação , Fluoresceínas/química , Fosfolipases A2 do Grupo II/antagonistas & inibidores , Fosfolipases A2 do Grupo II/genética , Humanos , Interações Hidrofóbicas e Hidrofílicas , Lipossomos/química , Modelos Moleculares , Simulação de Dinâmica Molecular , Mutagênese Sítio-Dirigida , Ligação Proteica , Conformação Proteica , Proteínas Recombinantes/antagonistas & inibidores , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Espectrometria de Fluorescência , Eletricidade Estática , Relação Estrutura-AtividadeRESUMO
The Human Secreted Group IIA Phospholipase A(2) (hsPLA2GIIA) presents potent bactericidal activity, and is considered to contribute to the acute-phase immune response. Hydrolysis of inner membrane phospholipids is suggested to underlie the bactericidal activity, and we have evaluated this proposal by comparing catalytic activity with bactericidal and liposome membrane damaging effects of the G30S, H48Q and D49K hsPLA2GIIA mutants. All mutants showed severely impaired hydrolytic activities against mixed DOPC:DOPG liposome membranes, however the bactericidal effect against Micrococcus luteus was less affected, with 50% killing at concentrations of 1, 3, 7 and 9 µg/mL for the wild-type, D49K, H48Q and G30S mutants respectively. Furthermore, all proteins showed Ca(2+)-independent damaging activity against liposome membranes demonstrating that in addition to the hydrolysis-dependent membrane damage, the hsPLA2GIIA presents a mechanism for permeabilization of phospholipid bilayers that is independent of catalytic activity, which may play a role in the bactericidal function of the protein.
Assuntos
Bactérias/metabolismo , Fosfolipases A2 do Grupo II/metabolismo , Sequência de Bases , Biocatálise , Domínio Catalítico , Primers do DNA , Fosfolipases A2 do Grupo II/genética , Humanos , Lipossomos , Mutagênese Sítio-Dirigida , Proteólise , Espectrofotometria UltravioletaRESUMO
Bothropstoxin-I (BthTx-I) is a Lys49-PLA(2) from the venom of the snake Bothrops jararacussu, which permeabilizes biological and artificial membranes by a mechanism independent of lipid hydrolysis. This mechanism has been investigated by studying the interaction of nine single tryptophan BthTx-I mutants with negatively charged phospholipid membranes. Changes in the solvent exposure of the tryptophan in each mutant were evaluated comparing the rate of chemical modification (k(mod)) by bromosuccinamide with the maximum intrinsic tryptophan fluorescence emission wavelength (lambda(max)) in buffer and in the presence of 10% DMPA/90% DPPC liposomes. No changes in lambda(max) were observed, whereas k(mod) values for tryptophans at positions 7, 10, 31 and 125 were significantly reduced in the presence of lipids, suggesting that bound phospholipid decreases solvent accessibility at these positions. Since the half-lives of the fluorescence and chemical modification effects differ by at least six orders of magnitude, these results suggest that the bound phospholipid may interact with multiple locations on the protein surface over micro- to millisecond timescales.
Assuntos
Venenos de Crotalídeos/farmacologia , Lipossomos/química , Animais , Bothrops , Cinética , Membranas Artificiais , Permeabilidade/efeitos dos fármacos , Fosfolipídeos/química , Triptofano/químicaRESUMO
The secreted phospholipases A2 (sPLA2s) are water-soluble enzymes that bind to the surface of both artificial and biological lipid bilayers and hydrolyze the membrane phospholipids. The tissue expression pattern of the human group IID secretory phospholipase A2 (hsPLA2-IID) suggests that the enzyme is involved in the regulation of the immune and inflammatory responses. With an aim to establish an expression system for the hsPLA2-IID in Escherichia coli, the DNA-coding sequence for hsPLA2-IID was subcloned into the vector pET3a, and expressed as inclusion bodies in E. coli (BL21). A protocol has been developed to refold the recombinant protein in the presence of guanidinium hydrochloride, using a size-exclusion chromatography matrix followed by dilution and dialysis to remove the excess denaturant. After purification by cation-exchange chromatography, far ultraviolet circular dichroism spectra of the recombinant hsPLA2-IID indicated protein secondary structure content similar to the homologous human group IIA secretory phospholipase A2. The refolded recombinant hsPLA2-IID demonstrated Ca(2+)-dependent hydrolytic activity, as measuring the release free fatty acid from phospholipid liposomes. This protein expression and purification system may be useful for site-directed mutagenesis experiments of the hsPLA2-IID which will advance our understanding of the structure-function relationship and biological effects of the protein.
Assuntos
Escherichia coli/enzimologia , Corpos de Inclusão/química , Fosfolipases A2/metabolismo , Cromatografia em Gel , Cromatografia por Troca Iônica , Dicroísmo Circular , Escherichia coli/química , Escherichia coli/genética , Guanidina/química , Humanos , Corpos de Inclusão/enzimologia , Fosfolipases A2/química , Fosfolipases A2/genética , Fosfolipases A2/isolamento & purificação , Fosfolipídeos/metabolismo , Engenharia de Proteínas , Dobramento de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismoRESUMO
Directed evolution techniques have been used to improve the thermal stability of the xylanase A from Bacillus subtilis (XylA). Two generations of random mutant libraries generated by error prone PCR coupled with a single generation of DNA shuffling produced a series of mutant proteins with increasing thermostability. The most Thermostable XylA variant from the third generation contained four mutations Q7H, G13R, S22P, and S179C that showed an increase in melting temperature of 20 degrees C. The thermodynamic properties of a representative subset of nine XylA variants showing a range of thermostabilities were measured by thermal denaturation as monitored by the change in the far ultraviolet circular dichroism signal. Analysis of the data from these thermostable variants demonstrated a correlation between the decrease in the heat capacity change (deltaC(p)) with an increase in the midpoint of the transition temperature (T(m)) on transition from the native to the unfolded state. This result could not be interpreted within the context of the changes in accessible surface area of the protein on transition from the native to unfolded states. Since all the mutations are located at the surface of the protein, these results suggest that an explanation of the decrease in deltaC(p) should include effects arising from the protein/solvent interface.
Assuntos
Bacillus subtilis/enzimologia , Evolução Molecular Direcionada , Endo-1,4-beta-Xilanases/química , Temperatura , Termodinâmica , Proteínas de Bactérias , Endo-1,4-beta-Xilanases/genética , Estabilidade Enzimática/genética , Mutação de Sentido Incorreto , Desnaturação Proteica/genéticaRESUMO
Bothropstoxin-I (BthTx-I) is a homodimeric Lys49-PLA2 from the venom of the snake Bothrops jararacussu, which lacks hydrolytic activity against phospholipid substrates, yet permeabilizes membranes by a Ca2+-independent mechanism. The interaction of the BthTx-I with model membranes has been studied by intrinsic tryptophan fluorescence emission (ITFE) spectroscopy. Nine separate mutants have been created each with a unique tryptophan residue located at a different position in the interfacial recognition site (IRS) of the protein. The rapid and efficient Ca2+-independent membrane damage against unilamellar liposomes composed of DPPC/DMPA in a 9:1 molar ratio was unaffected by these substitutions. Binding studies revealed low protein affinity for these liposomes and no changes were observed in the ITFE properties. In contrast, the binding of all mutants to DPPC/DMPA liposomes in a 1:1 molar ratio was stronger, and was correlated with altered ITFE properties. The blue-shifted emission spectra and increased emission intensity of mutants at positions 31, 67 and 115-117 in the interface recognition surface of the protein suggest these regions are partially inserted into the membrane. These results are consistent with a model for the Ca2+-independent membrane damaging mechanism that involves a transient interaction of the protein with the outer phospholipid leaflet of the target membrane.
Assuntos
Bothrops/metabolismo , Venenos de Crotalídeos/química , Venenos de Crotalídeos/metabolismo , Fosfolipases A2/metabolismo , Fosfolipídeos/química , Fosfolipídeos/metabolismo , Animais , Anisotropia , Bothrops/genética , Cálcio/metabolismo , Dicroísmo Circular , Venenos de Crotalídeos/genética , Lipossomos , Lisina/genética , Lisina/metabolismo , Modelos Moleculares , Fosfolipases A2/genética , Estrutura Quaternária de Proteína , Estrutura Secundária de Proteína , Triptofano/genética , Triptofano/metabolismoRESUMO
BthTx-I (bothropstoxin-I) is a myotoxic Lys49-PLA2 (phospholipase A2 with Lys49) isolated from Bothrops jararacussu venom, which damages liposome membranes by a Ca2+-independent mechanism. The highly conserved Phe5/Ala102/Phe106 motif in the hydrophobic substrate-binding site of the Asp49-PLA2s is substituted by Leu5/Val102/Leu106 in the Lys49-PLA2s. The Leu5/Val102/Leu106 triad in BthTx-I was sequentially mutated via all single- and double-mutant combinations to the Phe5/Ala102/Phe106 mutant. All mutants were expressed as inclusion bodies in Escherichia coli, and the thermal stability (Tm), together with the myotoxic and Ca2+-independent membrane-damaging activities of the recombinant proteins, were evaluated. The far-UV CD profiles of the native, wild-type recombinant and the L106F (Leu106-->Phe) and L5F/F102A/L106F mutant proteins were identical. The L5F, V102A, L5F/V102A and V102A/L106F mutants showed distorted far-UV CD profiles; however, only the L5F and L5F/V102A mutants showed significant decreases in Tm. Alterations in the far-UV CD spectra correlated with decreased myotoxicity and protein-induced release of a liposome-entrapped marker. However, the V102A/L106F and L5F/V102A/L106F mutants, which presented high myotoxic activities, showed significantly reduced membrane-damaging activity. This demonstrates that the topology of the substrate-binding region of BthTx-I has a direct effect on the Ca2+-independent membrane damage, and implies that substrate binding retains an important role in this process.
Assuntos
Cálcio/metabolismo , Membranas Intracelulares/metabolismo , Lisina/química , Fosfolipases A/química , Animais , Sítios de Ligação , Bothrops/metabolismo , Dicroísmo Circular/métodos , Venenos de Crotalídeos/química , Venenos de Crotalídeos/genética , Venenos de Crotalídeos/farmacologia , Cristalografia por Raios X/métodos , Escherichia coli K12/genética , Escherichia coli K12/metabolismo , Membranas Intracelulares/efeitos dos fármacos , Lipossomos/química , Lipossomos/metabolismo , Lisina/genética , Lisina/farmacologia , Mutagênese Sítio-Dirigida/genética , Neurotoxinas/química , Neurotoxinas/genética , Neurotoxinas/farmacologia , Fosfolipases A/genética , Fosfolipases A/farmacologia , Fosfolipases A2 , Ligação Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/farmacologia , Proteínas de Répteis , Venenos de Serpentes/química , Venenos de Serpentes/metabolismo , Venenos de Serpentes/farmacologia , Especificidade por SubstratoRESUMO
Bothropstoxin I (4BthTx-I) is a homodimeric lysine-49 (Lys49) phospholipase A(2) isolated from Bothrops jararacussu venom, which damages liposome membranes via a Ca(2+)-independent mechanism. The stability of the BthTx-I homodimer was evaluated by equilibrium chemical denaturation with guanidinium hydrochloride monitored by changes in the intrinsic tryptophan fluorescence anisotropy, far-UV circular dichroism, dynamic light scattering, and 1-anilinonaphthalene-8-sulfonate binding. Unfolding of the BthTx-I dimer proceeds via a monomeric intermediate with native-like structure, with Gibbs free energy (DeltaG(0)) values of 10.0 and 7.2 kcal mol(-1) for the native dimer-to-native monomer and native-to-denatured monomer transitions, respectively. The experimentally determined DeltaG(0) value for the dimer-to-native monomer transition is higher than the value expected for an interaction dominated by hydrophobic forces, and suggests that an unusually high propensity of hydrogen-bonded side chains found at the BthTx-I homodimer interface make a significant contribution to dimer stability.