RESUMO
Arbuscular mycorrhizal (AM) fungi and plant growth-promoting rhizobacteria (PGPR) are beneficial microorganisms that may associate with grapevine roots, improving stress tolerance, growth, and nutrition. AM fungi and PGPR enhance the production of plant secondary metabolites, including volatile organic compounds (VOCs) that play a key role in the interaction of plants with the environment and are involved in defence mechanisms. The aim of this study was to analyse the effects of an AM fungus and a rhizobacterium on plant growth and VOCs in Vitis vinifera cv. Cabernet Sauvignon roots to gain insight into the potential role of plant-rhizosphere microorganisms in vine growth and defence. Grapevines were inoculated or not with the AM fungus Funneliformis mosseae IN101 and/or the plant growth-promoting rhizobacterium Ensifer meliloti TSA41. Both microbial strains enhanced plant growth. Fifty-eight VOCs extracted from ground roots were identified using headspace solid-phase microextraction coupled to gas chromatography/mass spectrometry. VOCs were induced by F. mosseae IN101, increasing up to 87% compared with control plants. Monoterpenes were strongly enhanced by F. mosseae IN101, increasing up to 113% compared with control plants. Interestingly, monoterpene alcohols related to plant defence, such as myrtenol, p-cymen-7-ol, and p-mentha-1.8-dien-7-ol were increased. By contrast, E. meliloti TSA41 did not significantly affect VOCs. The knowledge of the effects of AM fungi and PGPR on grapevine VOCs may contribute to an integrated and sustainable management of vineyards.
Assuntos
Glomeromycota , Micorrizas , Vitis , Compostos Orgânicos Voláteis , Raízes de PlantasRESUMO
Inoculation with Azospirillum brasilense Sp245 exerts beneficial effects on micropropagated plants of Prunus cerasifera L. clone Mr.S 2/5, as seen in the results of a comparative analysis of inoculated and non-inoculated explants, during both the rooting and acclimatation phases. The presence of Azospirillum brasilense Sp245 increased root system, root hair biomass production and apical activity. Although the presence of the bacteria had a positive effect on rooting, the addition of indolebutyric acid (IBA) to Murashige and Skoog (MS) medium was seen as indispensable in order to promote the rooting of explants. Aside from the promotion of plant growth, A. brasilense Sp245 protects plants against pathogen attacks, such as Rhizoctonia spp., with a plant survival rate of nearly 100% vs. 0% as seen in the negative control. The biocontrol effect of A. brasilense Sp245 on the fungal rhizospheric community has been confirmed by denaturing gradient gel electrophoresis (DGGE) profiles of the rhizospheric microbial community. This study indicates that A. brasilense Sp245 could be employed as a tool in plant biotechnology.