RESUMO
Rosmarinus officinalis L. (Lamiaceae), popularly known as rosemary, is used for food flavoring and in folk medicine as an antispasmodic, analgesic, antirheumatic, diuretic, and antiepileptic agent. Few studies have shown the anti-inflammatory effects of rosemary essential oil (REO). This study evaluated the effects of REO on leukocyte migration through in vivo leukocyte migration and in vitro chemotaxis assay. REO was analyzed by using gas chromatography-mass spectometry, and the main components identified were camphor (27.59%), 1,8-cineole (15.74%), α-pinene (16.58%), and ß-myrcene (10.02%). In rats, administration of REO reduced the number of leukocytes that rolled, adhered, and migrated to the scrotal chamber after carrageenan injection. All doses of REO tested significantly inhibited leukocyte chemotaxis induced by casein. The effects of REO on leukocyte migration highlight an important mechanism of the anti-inflammatory action of rosemary.
Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Inibição de Migração Celular , Quimiotaxia de Leucócito/efeitos dos fármacos , Leucócitos/efeitos dos fármacos , Óleos Voláteis/farmacologia , Animais , Anti-Inflamatórios não Esteroides/administração & dosagem , Anti-Inflamatórios não Esteroides/química , Carragenina/toxicidade , Ensaios de Migração de Leucócitos , Relação Dose-Resposta a Droga , Endotélio/citologia , Endotélio/efeitos dos fármacos , Endotélio/imunologia , Cromatografia Gasosa-Espectrometria de Massas , Teste de Inibição de Aderência Leucocítica , Leucócitos/citologia , Leucócitos/imunologia , Espectroscopia de Ressonância Magnética , Masculino , Medicina Tradicional , Monoterpenos/análise , Monoterpenos/química , Monoterpenos/farmacologia , Óleos Voláteis/administração & dosagem , Óleos Voláteis/química , Folhas de Planta/química , Ratos , Ratos Wistar , Rosmarinus/química , Escroto/citologia , Escroto/efeitos dos fármacos , Escroto/imunologiaRESUMO
Zingiber officinale Roscoe, popular name ginger, is grown naturally in many parts of the world, including Brazil. Ginger is used in pharmaceutical, cosmetic, and food and beverage industries and the essential oil has been used in folk medicine for manifold conditions including as an analgesic, anti-inflammatory, and antirheumatic. The purpose of this study was to investigate the effects of ginger (Zingiber officinale Roscoe) essential oil (GEO) in an in vitro chemotaxis assay and on leukocyte-endothelial interactions in vivo. GEO was analyzed by GC-MS and the main components identified were ar-curcumene (59%), ß-myrcene (14%), 1,8-cineol (8%), citral (7.5%), and zingiberene (7.5%). Oral administration of GEO (200-500 mg/kg) reduced the rolling and leukocyte adherence after 2 h of carrageenan injection (100 µg) into the scrotal chamber. The number of leukocytes migrated to the perivascular tissue 4 h after the irritant stimulus was also diminished. GEO in all doses tested (10(-4), 10(-3), or 10(-2) µL/mL) caused a significant reduction of leukocyte chemotaxis (35.89 ± 4.33, 30.67 ± 0.70, and 35.85 ± 3.83%, respectively) toward casein stimuli. The data presented showed direct and systemic effects of GEO on leukocyte migration as an important mechanism of the anti-inflammatory action of ginger.