RESUMO

Increasing efforts are directed toward finding applications for natural products and their derivatives in the treatment of human diseases. Among such products, propolis, a resinous substance produced by honey bees from various plant sources, has been found to be a promising source of potential therapeutics. In the present work, we aimed at studying the perspective of Cuban propolis as a source of possible anti-cancer agents. We found an anti-metastatic effect in mice and considerable cytotoxicity without cross-resistance in both wild-type and chemoresistant human tumor cell lines. Plukenetione A--identified for the first time in Cuban propolis--induced G0/G1 arrest and DNA fragmentation in colon carcinoma cells. Furthermore, the activities of both topoisomerase I and DNA polymerase were inhibited, while the expression of topoisomerase II-beta, EGF receptor, and multidrug resistance-related protein genes was found repressed. We assume that plukenetione A contributes to the anti-tumoral effect of Cuban propolis mainly by targeting topoisomerase I as well as DNA polymerase.

Assuntos

Antineoplásicos/farmacologia , Compostos Policíclicos/farmacologia , Própole/química , Animais , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Ciclo Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Cuba , Dano ao DNA/efeitos dos fármacos , DNA Polimerase III/antagonistas & inibidores , DNA Polimerase III/genética , DNA Polimerase III/metabolismo , DNA Topoisomerases Tipo I/genética , DNA Topoisomerases Tipo I/metabolismo , Feminino , Fase G1/efeitos dos fármacos , Perfilação da Expressão Gênica , Humanos , Concentração Inibidora 50 , Camundongos , Compostos Policíclicos/química , Compostos Policíclicos/isolamento & purificação , Própole/farmacologia , Fase de Repouso do Ciclo Celular/efeitos dos fármacos , Inibidores da Topoisomerase I , Células Tumorais Cultivadas