RESUMO
This study presents the design and development of an ultrasonic sensor as a fundamental tool for characterizing the properties of fluids and biofluids. The analysis primarily focuses on measuring the electrical parameters of the system, which correlate with the density and viscosity of the solutions, in sample volumes of microliters and with high temporal resolution (up to 1 data point per second). The use of this sensor allows the fast and non-destructive evaluation of the viscosity and density of fluids deposited on its free surface. The measurements are based on obtaining the impedance versus frequency curve and the phase difference curve (between current and voltage) versus frequency. In this way, characteristic parameters of the transducer, such as the resonance frequency, phase, minimum impedance, and the quality factor of the resonant system, can characterize variations in density and viscosity in the fluid under study. The results obtained revealed the sensor's ability to identify two parameters sensitive to viscosity and two parameters sensitive to density. As a proof of concept, the unfolding of the bovine albumin protein was studied, resulting in a curve that reflects its unfolding kinetics in the presence of urea.
Assuntos
Técnicas Biossensoriais , Viscosidade , Soroalbumina Bovina/química , Animais , Bovinos , UltrassomRESUMO
We report a new oscillatory propagation of cracks in thin films where three cracks interact mediated by two delamination fronts. Experimental observations indicate that delamination fronts joining the middle crack to the lateral crack tips swap contact periodically with the crack tip of the middle crack. A model based on a variational approach analytically predicts the condition of propagation and geometrical features of three parallel cracks. The stability conditions and oscillating propagation are found numerically and the predictions are in favorable agreement with experiments. We found that the physical mechanism selecting the wavelength structure is a relaxation process in which the middle crack produces a regular oscillatory path.
RESUMO
We study multiple tearing of a thin, elastic, brittle sheet indented with a rigid cone. The n cracks initially prepared symmetrically propagate radially for n≥4. However, if n<4 the radial symmetry is broken and fractures spontaneously intertwine along logarithmic spiral paths, respecting order n rotational symmetry. In the limit of very thin sheets, we find that fracture mechanics is reduced to a geometrical model that correctly predicts the maximum number of spirals to be strictly 4, together with their growth rate and the perforation force. Similar spirals are also observed in a different tearing experiment (this time up to n=4, in agreement with the model), in which bending energy of the sheet is dominant.