RESUMO
The results of a previous study suggested that Cherrie's cane rat (Zygodontomys cherriei) is the principal host of Necoclí virus (family Bunyaviridae, genus Hantavirus) in Colombia. Bayesian analyses of complete nucleocapsid protein gene sequences and complete glycoprotein precursor gene sequences in this study confirmed that Necoclí virus is phylogenetically closely related to Maporal virus, which is principally associated with the delicate pygmy rice rat (Oligoryzomys delicatus) in western Venezuela. In pairwise comparisons, nonidentities between the complete amino acid sequence of the nucleocapsid protein of Necoclí virus and the complete amino acid sequences of the nucleocapsid proteins of other hantaviruses were ≥8.7%. Likewise, nonidentities between the complete amino acid sequence of the glycoprotein precursor of Necoclí virus and the complete amino acid sequences of the glycoprotein precursors of other hantaviruses were ≥11.7%. Collectively, the unique association of Necoclí virus with Z. cherriei in Colombia, results of the Bayesian analyses of complete nucleocapsid protein gene sequences and complete glycoprotein precursor gene sequences, and results of the pairwise comparisons of amino acid sequences strongly support the notion that Necoclí virus represents a novel species in the genus Hantavirus. Further work is needed to determine whether Calabazo virus (a hantavirus associated with Z. brevicauda cherriei in Panama) and Necoclí virus are conspecific.
Assuntos
Doenças Transmissíveis/epidemiologia , Infecções por Hantavirus/epidemiologia , Orthohantavírus/classificação , Sigmodontinae/virologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Teorema de Bayes , Colômbia/epidemiologia , Doenças Transmissíveis/virologia , Orthohantavírus/genética , Orthohantavírus/isolamento & purificação , Infecções por Hantavirus/virologia , Dados de Sequência Molecular , Proteínas do Nucleocapsídeo/genética , Filogenia , Análise de Sequência de DNA , Venezuela/epidemiologiaRESUMO
To increase our knowledge of the geographic distribution of hantaviruses associated with neotomine or sigmodontine rodents in Mexico, we tested 876 cricetid rodents captured in 18 Mexican states (representing at least 44 species in the subfamily Neotominae and 10 species in the subfamily Sigmodontinae) for anti-hantavirus IgG. We found antibodies against hantavirus in 35 (4.0%) rodents. Nucleotide sequence data from 5 antibody-positive rodents indicated that Sin Nombre virus (the major cause of hantavirus pulmonary syndrome [HPS] in the United States) is enzootic in the Mexican states of Nuevo León, San Luis Potosí, Tamaulipas, and Veracruz. However, HPS has not been reported from these states, which suggests that in northeastern Mexico, HPS has been confused with other rapidly progressive, life-threatening respiratory diseases. Analyses of nucleotide sequence data from 19 other antibody-positive rodents indicated that El Moro Canyon virus and Limestone Canyon virus are geographically widely distributed in Mexico.
Assuntos
Infecções por Hantavirus/veterinária , Doenças dos Roedores/virologia , Sigmodontinae/virologia , Animais , Anticorpos Antivirais/sangue , Teorema de Bayes , Orthohantavírus/genética , Infecções por Hantavirus/epidemiologia , Infecções por Hantavirus/imunologia , Imunoglobulina G/sangue , Pulmão/virologia , México/epidemiologia , Modelos Genéticos , Proteínas do Nucleocapsídeo/genética , Filogenia , Doenças dos Roedores/epidemiologia , Doenças dos Roedores/imunologia , Análise de Sequência de DNA , Sigmodontinae/imunologia , Proteínas Virais de Fusão/genéticaRESUMO
Arenavirus RNA was isolated from Mexican deer mice (Peromyscus mexicanus) captured near the site of a 1967 epidemic of hemorrhagic fever in southern Mexico. Analyses of nucleotide and amino acid sequence data indicated that the deer mice were infected with a novel Tacaribe serocomplex virus (proposed name Ocozocoautla de Espinosa virus), which is phylogenetically closely related to Tacaribe serocomplex viruses that cause hemorrhagic fever in humans in South America.
Assuntos
Arenavirus do Novo Mundo/isolamento & purificação , Febre Hemorrágica Americana/epidemiologia , Animais , Arenavirus do Novo Mundo/classificação , Arenavirus do Novo Mundo/genética , Febre Hemorrágica Americana/diagnóstico , Febre Hemorrágica Americana/virologia , Humanos , México/epidemiologia , Dados de Sequência Molecular , Proteínas do Nucleocapsídeo/genética , Peromyscus/virologia , Filogenia , Homologia de SequênciaRESUMO
Samples from rodents captured on a farm in Venezuela in February 1997 were tested for arenavirus, antibody against Guanarito virus (GTOV), and antibody against Pirital virus (PIRV). Thirty-one (48.4%) of 64 short-tailed cane mice (Zygodontomys brevicauda) were infected with GTOV, 1 Alston's cotton rat (Sigmodon alstoni) was infected with GTOV, and 36 (64.3%) of 56 other Alston's cotton rats were infected with PIRV. The results of analyses of field and laboratory data suggested that horizontal transmission is the dominant mode of GTOV transmission in Z. brevicauda mice and that vertical transmission is an important mode of PIRV transmission in S. alstoni rats. The results also suggested that bodily secretions and excretions from most GTOV-infected short-tailed cane mice and most PIRV-infected Alston's cotton rats may transmit the viruses to humans.
Assuntos
Infecções por Arenaviridae/veterinária , Arvicolinae/virologia , Animais , Animais Selvagens/virologia , Anticorpos Antivirais/sangue , Infecções por Arenaviridae/epidemiologia , Infecções por Arenaviridae/transmissão , Arenavirus/classificação , Arenavirus/genética , Arenavirus/imunologia , Doenças Transmissíveis Emergentes/epidemiologia , Doenças Transmissíveis Emergentes/transmissão , Doenças Transmissíveis Emergentes/veterinária , Feminino , Humanos , Masculino , Filogenia , Venezuela/epidemiologia , Eliminação de Partículas Virais , Zoonoses/transmissãoRESUMO
Blood samples from 4893 cricetid rodents were tested for antibody (immunoglobulin G) to Whitewater Arroyo virus and Amaparí virus to extend our knowledge of the natural host range and geographical distribution of Tacaribe serocomplex viruses in North America. Antibodies to arenaviruses were found in northern pygmy mice (Baiomys taylori), woodrats (Neotoma spp.), northern grasshopper mice (Onychomys leucogaster), oryzomys (Oryzomys spp.), deermice (Megadontomys nelsoni and Peromyscus spp.), harvest mice (Reithrodontomys spp.), and cotton rats (Sigmodon spp.) captured in New Mexico, Texas, or Mexico. Comparison of endpoint antibody titers to Whitewater Arroyo virus and Amaparí virus in individual blood samples indicated that the Tacaribe complex viruses enzootic in Texas and Mexico are antigenically diverse.
Assuntos
Infecções por Arenaviridae/veterinária , Arvicolinae , Animais , Anticorpos Antivirais/sangue , Infecções por Arenaviridae/sangue , Infecções por Arenaviridae/imunologia , Arenavirus/imunologia , Arvicolinae/sangue , México , New Mexico/epidemiologia , Texas/epidemiologiaRESUMO
Hantavirus HTN.007 was originally isolated from a small-eared pygmy rice rat (Oligoryzomys microtis) captured in northeastern Peru. The results of analyses of nucleotide and amino acid sequence data in this study indicated that HTN.007 is a strain of Rio Mamoré virus (RIOMV) which is enzootic in small-eared pygmy rice rat populations in Bolivia. As such, the results of this study extend our knowledge of the geographical range of RIOMV and support the notion that the small-eared pygmy rice rat is the principal host of RIOMV.
Assuntos
Orthohantavírus/classificação , Orthohantavírus/fisiologia , Sigmodontinae/virologia , Animais , Proteínas do Capsídeo/genética , Regulação Viral da Expressão Gênica , Infecções por Hantavirus/epidemiologia , Infecções por Hantavirus/veterinária , Infecções por Hantavirus/virologia , Interações Hospedeiro-Patógeno , Peru/epidemiologia , Filogenia , Filogeografia , Doenças dos Roedores/epidemiologia , Doenças dos Roedores/virologia , Proteínas do Core Viral/genéticaRESUMO
We isolated arenavirus RNA from white-toothed woodrats (Neotoma leucodon) captured in a region of Mexico in which woodrats are food for humans. Analyses of nucleotide and amino acid sequence data indicated that the woodrats were infected with a novel Tacaribe serocomplex virus, proposed name Real de Catorce virus.
Assuntos
Infecções por Arenaviridae/veterinária , Arenavirus do Novo Mundo , Doenças dos Roedores/virologia , Animais , Anticorpos Antivirais/análise , Infecções por Arenaviridae/epidemiologia , Infecções por Arenaviridae/imunologia , Infecções por Arenaviridae/virologia , Arenavirus do Novo Mundo/genética , Arenavirus do Novo Mundo/isolamento & purificação , Genoma Viral , Rim/imunologia , Rim/virologia , México/epidemiologia , Filogenia , RNA Viral/genética , RNA Viral/isolamento & purificação , Doenças dos Roedores/epidemiologia , Doenças dos Roedores/imunologia , Roedores , Análise de Sequência de RNA , Baço/imunologia , Baço/virologiaRESUMO
Strains of Caño Delgadito virus (CADV) and Maporal virus (MAPV) were isolated from 25 (8.9%) of the 280 rodents captured on farms in 1997 in western Venezuela. The results of analyses of laboratory and zoographic data indicated that Alston's cotton rat (Sigmodon alstoni) is the principal host of CADV, horizontal virus transmission is the dominant mode of CADV transmission in Alston's cotton rat in nature, a pygmy rice rat (Oligoryzomys sp.) is the principal host of MAPV, and the natural host relationships of CADV and MAPV are highly specific.
Assuntos
Orthohantavírus/isolamento & purificação , Roedores/virologia , Animais , Feminino , Orthohantavírus/fisiologia , Infecções por Hantavirus/epidemiologia , Infecções por Hantavirus/veterinária , Infecções por Hantavirus/virologia , Interações Hospedeiro-Patógeno , Masculino , Especificidade da Espécie , Venezuela/epidemiologiaRESUMO
Machupo virus and Chapare virus are members of the Tacaribe serocomplex (virus family Arenaviridae) and etiological agents of hemorrhagic fever in humans in Bolivia. The nucleotide sequences of the complete Z genes, a large fragment of the RNA-dependent RNA polymerase genes, the complete glycoprotein precursor genes, and the complete nucleocapsid protein genes of 8 strains of Machupo virus were determined to increase our knowledge of the genetic diversity among the Bolivian arenaviruses. The results of analyses of the predicted amino acid sequences of the glycoproteins of the Machupo virus strains and Chapare virus strain 200001071 indicated that immune plasma from hemorrhagic fever cases caused by Machupo virus may prove beneficial in the treatment of Bolivian hemorrhagic fever but not hemorrhagic fever caused by Chapare virus.
Assuntos
Arenavirus do Novo Mundo/genética , Variação Genética , RNA Viral/genética , Sequência de Aminoácidos , Animais , Infecções por Arenaviridae/virologia , Arenavirus do Novo Mundo/classificação , Bolívia , Chlorocebus aethiops , Evolução Molecular , Glicoproteínas/genética , Febre Hemorrágica Americana/virologia , Humanos , Proteínas do Nucleocapsídeo/genética , Filogenia , RNA Polimerase Dependente de RNA/genética , Alinhamento de Sequência , Análise de Sequência de RNA , Especificidade da Espécie , Células Vero , Proteínas do Envelope Viral/genéticaRESUMO
The results of analyses of Z, RNA-dependent RNA polymerase, glycoprotein precursor, and nucleocapsid protein gene sequence data suggested that Guanarito virus was the most common cause of Venezuelan hemorrhagic fever in a 7-year period in the 1990s and that the evolution of Pirital virus in association with Sigmodon alstoni (Alston's cotton rat) has occurred at a significantly higher rate than the evolution of Guanarito virus in association with Zygodontomys brevicauda (short-tailed cane mouse) on the plains of western Venezuela. The results of analyses of the primary structures of the glycoproteins of the 8 strains of Guanarito virus isolated from humans suggested that these strains would be highly cross-reactive in neutralization assays. Thus, passive antibody therapy may prove beneficial in the treatment of human disease caused by strains of Guanarito virus that are enzootic in the region in which Venezuelan hemorrhagic fever is endemic.