RESUMO
The liver is primarilyresponsible for energy homeostasis and the regulation of lipid, carbohydrate and protein metabolism. Lipid metabolism consists of distributing lipids to peripheral tissues or ensuring their return to the liver to be reprocessed. Additionally, cellular metabolism isregulated by several molecules in different signaling pathways. Lipid homeostasis in the liver is mainly regulated by AKT, AMPK, SREBP, PPAR, and JNK. The PI3K/AKT/mTOR signaling pathway results in the biosynthesis of macromolecules and regulates lipogenesis and the expression of lipogenic genes. AMPK is an energy sensor that regulates metabolism and is activated when stored ATP is depleted, and it is responsible for the suppression of several key lipogenic factors in the liver related to cholesterol and fatty acid synthesis. SREBPs control lipogenic geneexpressionandcholesterol metabolism and actin the nutritional regulation of fatty acids and triglycerides. The continued activation of SREBPs is associated with cellular stress, inflammation and ultimately steatosis. PPARs are intrinsically important regulators of lipid metabolism. These genes are essential tovarious metabolic processes, especially lipid and glucose homeostasis, and can play a role in cell differentiation. JNK signaling is related to insulin resistance and its activation results in decreased mitochondrial activity and fat accumulation. Therefore, the study of cell signaling pathways related to lipid metabolism and liver function may help to identify abnormalities and develop strategies to manage and regulate metabolic disorders and resulting complications.
Assuntos
Fígado Gorduroso/metabolismo , Fígado/fisiologia , Fígado/metabolismo , Metabolismo dos Lipídeos , RanunculaceaeRESUMO
Barrier membranes for guided bone regeneration (GBR) mainly promote mechanical maintenance of bone defect space and induce osteopromotion. Additionally, biopolymer-based membranes may provide greater bioactivity and biocompatibility due to their similarity to extracellular matrix (ECM). In this study, biopolymers-based membranes from bacterial cellulose (BC) and collagen (COL) associated with osteogenic growth peptide (OGP(10-14)) were evaluated to determine in vitro osteoinductive potential in early osteogenesis; moreover, histological study was performed to evaluate the BC-COL OGP(10-14) membranes on bone healing after GBR in noncritical defects in rat femur. The results showed that the BC-COL and BC-COL OGP(10-14) membranes promoted cell proliferation and alkaline phosphatase activity in osteoblastic cell cultures. However, ECM mineralization was similar between cultures grown on BC OGP(10-14) and BC-COL OGP(10-14) membranes. In vivo results showed that all the membranes tested, including the peptide-free BC membrane, promoted better bone regeneration than control group. Furthermore, the BC-COL OGP(10-14) membranes induced higher radiographic density in the repaired bone than the other groups at 1, 4 and 16 weeks. Histomorphometric analyses revealed that the BC-COL OGP(10-14) induced higher percentage of bone tissue in the repaired area at 2 and 4 weeks than others membranes. In general, these biopolymer-based membranes might be potential candidates for bone regeneration applications.
Assuntos
Biopolímeros/química , Regeneração Óssea , Histonas/química , Peptídeos e Proteínas de Sinalização Intercelular/química , Osteogênese/efeitos dos fármacos , Animais , Animais Recém-Nascidos , Osso e Ossos/patologia , Técnicas de Cultura de Células , Membrana Celular/metabolismo , Proliferação de Células , Colágeno/química , Masculino , Osteoblastos/citologia , Peptídeos/química , Polímeros/química , Ratos , Ratos WistarRESUMO
Objective. To describe the development of the bacterial cellulose coating with anti-inflammatory Ibuprofen (BC/Ibu) and to evaluate the cicatrization process with its use in patients with chronic wounds of venous and diabetic etiology. Methods. Longitudinal descriptive study. The cellulose membrane, cultivated with bacteria Gluconacetobacter xylinus and with incorporation of Ibuprofen, was used in the treatment of patients with chronic wounds in public health services in a Brazilian municipality. The ideal coverage characteristics were evaluated through physical, chemical and cell proliferation tests. Results. The sample consisted of 14 patients (10 women and 4 men), 8 with venous ulcer, 5 with diabetic foot and one with mixed wound. There was reduction of area and pain in 9 lesions; total healing of 3 wounds; and debridement of the devitalized tissue in 5 wounds with increased area. The use of the membrane was important in the reduction of pain, exudation and ease in the accomplishment of the curative. Conclusion. BC/Ibu favored the cicatrization process of patients with chronic vasculogenic wounds.(AU)
Objetivo. Describir el desarrollo de la cobertura de celulosa bacteriana con antiinflamatorio Ibuprofeno (CB/Ibu) y evaluar el proceso de cicatrización en la utilización en pacientes con heridas crónicas de etiología venosa y diabética. Métodos. Estudio descriptivo longitudinal. La membrana de celulosa, cultivada con bacterias Gluconacetobacter xylinus y con incorporación del Ibuprofeno se utilizó en el tratamiento de pacientes con heridas crónicas en servicios de atención pública de un municipio brasileño. Se evaluaron características de cobertura ideal mediante pruebas físicas, químicas y de proliferación celular. Resultados. La muestra fue constituida por 14 pacientes (10 mujeres y 4 hombres): 8 con úlcera venosa, 5 con pie diabético y uno con herida mixta. Se redujo el área y el dolor en 9 lesiones; la cicatrización total de 3 heridas; y el debridamiento del tejido desvitalizado en 5 heridas con aumento del área. El uso de la membrana de celulosa disminuyó el dolor de la exudación y facilitó la realización del vendaje. Conclusión. La CB/Ibu favoreció el proceso de cicatrización de los pacientes con heridas crónicas vasculogénicas.(AU)
Objetivo. Descrever o desenvolvimento da cobertura de celulose bacteriana com anti-inflamatório Ibuprofeno (CB/Ibu) e avaliar o processo de cicatrização com a sua utilização em pacientes com feridas crônicas de etiologia venosa e diabética. Métodos. Estudo descritivo longitudinal. A membrana de celulose, cultivada com bactérias Gluconacetobacter xylinus e com incorporação do Ibuprofeno, foi utilizada no tratamento de pacientes com feridas crônicas em serviços de atendimento público de um município brasileiro. Foram avaliadas as características de cobertura ideal mediante testes físicos, químicos e de proliferação celular. Resultados. A amostra foi constituída por 14 pacientes (10 mulheres e 4 homens), sendo 8 com úlcera venosa, 5 com pé diabético e um com ferida mista. Houve redução da área e da dor em 9 lesões; cicatrização total de 3 feridas; e o debridamento do tecido desvitalizado em 5 feridas com aumento da área. O uso da membrana além da diminuição da dor, da exsudação e facilidade na realização do curativo. Conclusão. A CB/Ibu favoreceu o processo cicatrização dos pacientes com feridas crônicas vasculogênicas. (AU)
Assuntos
Humanos , Úlcera Varicosa , Cicatrização , Celulose , Ibuprofeno , Pé Diabético , Gluconacetobacter xylinusRESUMO
Among remarkable discoveries concerning propolis, such as antifungal, antiviral, and antioxidant activities, its anti-inflammatory, and mainly its antibacterial, properties deserve special attention when skin wound healing is concerned. Based on this and knowing the distinctive performance of bacterial (BC) membranes on wound healing, in this work it is proposed to demonstrate the potent antimicrobial activity and wound healing properties of a novel propolis containing biocellulose membrane. The obtained propolis/BC membrane was able to adsorb propolis not only on the surface, but also in its interstices demonstrated by scanning electron microscopy, X-ray diffraction, Fourier transform infrared (FT-IR) spectroscopy, and thermogravidimetric assays. Additionally, the polyphenolic compounds determination and the prominent antibacterial activity in the membrane are demonstrated to be dose dependent, supporting the possibility of obtaining propolis/BC membranes at the desired concentrations, taking into consideration its application and its skin residence time. Finally, it could be suggested that propolis/BC membrane may favor tissue repair in less time and more effectively in contaminated wounds.
RESUMO
No estudo das más oclusões, podem estar presentes tanto alterações dentárias como esqueléticas. Nos casos de má oclusão Classe III pode-se observar retrusão maxilar, protrusão mandibular ou a combinação destes sinais, e, muitas vezes associadas a uma atresia maxilar que geralmente se manifesta por mordidas cruzadas anteriores, posteriores, uni ou bilaterais. Este relato de caso clínico tem como objetivo avaliar os efeitos no perfil facial e na oclusão da terapia ortodôntica interceptativa. O tratamento realizado com expansão do arco superior e protração maxilar foi executado em idade bastante jovem para que atuasse efetivamente no sistema sutural circumaxilar e o efeito ortopédico fosse facilitado. A máscara facial utilizada apoia-se em dois pontos da face e permite um reposicionamento maxilar anterior. A colaboração do paciente é importante para alcançar o sucesso do tratamento, isto porque o tempo de uso da máscara ainda nesta fase de crescimento e desenvolvimento é primordial para que os efeitos faciais e oclusais aconteçam. É importante ressaltar que os pacientes com má oclusão de Angle Classe III devem ser acompanhados até o final do crescimento...
In malocclusion studies, may happen not only dental discrepancies but even skeletal discrepancies. In Class III malocclusion can be observed underdeveloped maxilla, mandible protraction or both of them, and, in most of the times associated with a narrow maxilla that causes anterior or posterior crossbites, in one side or in two sides. The aim of this case report is to evaluate the facial profile and occlusal effects of interceptive orthodontics therapy. The treatment with upper arch expansion and maxilla protraction was done in an early age to be effective in the circummaxillary sutural system and to make the orthopedic effect easier. The facial mask used is positioned on two facial points and allows an anterior maxillary replacement. Patients' compliance is important to reach treatment success, this because using the mask still in this growth and development phase is essential to make the facial and occlusal effects happen. It is important to mention that patients with Angle Class III malocclusion need to be followed till the end of their growth...
Assuntos
Humanos , Masculino , Recém-Nascido , Diagnóstico , Má Oclusão Classe III de Angle , Ortodontia InterceptoraRESUMO
The aim of this study was to characterize the physicochemical properties of bacterial cellulose (BC) membranes functionalized with osteogenic growth peptide (OGP) and its C-terminal pentapeptide OGP[10-14], and to evaluate in vitro osteoinductive potential in early osteogenesis, besides, to evaluate cytotoxic, genotoxic and/or mutagenic effects. Peptide incorporation into the BC membranes did not change the morphology of BC nanofibers and BC crystallinity pattern. The characterization was complemented by Raman scattering, swelling ratio and mechanical tests. In vitro assays demonstrated no cytotoxic, genotoxic or mutagenic effects for any of the studied BC membranes. Culture with osteogenic cells revealed no difference in cell morphology among all the membranes tested. Cell viability/proliferation, total protein content, alkaline phosphatase activity and mineralization assays indicated that BC-OGP membranes enabled the highest development of the osteoblastic phenotype in vitro. In conclusion, the negative results of cytotoxicity, genotoxicity and mutagenicity indicated that all the membranes can be employed for medical supplies, mainly in bone tissue engineering/regeneration, due to their osteoinductive properties.
Assuntos
Osso e Ossos/efeitos dos fármacos , Celulose/química , Histonas/farmacologia , Peptídeos e Proteínas de Sinalização Intercelular/farmacologia , Membranas Artificiais , Engenharia Tecidual/métodos , Animais , Animais Recém-Nascidos , Bactérias/química , Osso e Ossos/fisiologia , Células CHO , Células Cultivadas , Celulose/isolamento & purificação , Celulose/farmacologia , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Cricetinae , Cricetulus , Osteogênese/efeitos dos fármacos , Ratos , Ratos WistarRESUMO
BACKGROUND: Tissue adhesives have been widely used for wound closure, especially in children, because they are painless, fast, and easy to use and result in minimal scarring. OBJECTIVE: To analyze the biocompatibility of an adhesive based on n-butyl-cyanoacrylate in the subcutaneous tissue of rats. MATERIALS AND METHODS: Two surgical sites were prepared (approximately 3 cm apart): one on the left side of the animal and the other on the right side); polyethylene tubes were implanted in each surgical site. The tube on the left was filled with n-butyl-cyanoacrylate (treated group) and the tube on the right side was unfilled (control group). After 7, 30, and 120 days, the animals were killed, and the specimens were processed for histologic analysis. RESULTS: No significant inflammatory reaction occurred in the treated group, showing results similar to the control group. CONCLUSION: This adhesive based on n-butyl-cyanoacrylate is biocompatible in the subcutaneous tissue of rats.
Assuntos
Embucrilato/farmacologia , Tela Subcutânea/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Animais , Materiais Biocompatíveis/farmacologia , Teste de Materiais , Ratos , Tela Subcutânea/cirurgiaRESUMO
A necrose pulpar representa a cessação dos processos metabólicos do tecido pulpar, desestruturação e concomitante perda de suas defesas naturais. A partir da necrose pulpar, a cavidade pulpar torna-se progressivamente colonizada por vários micro-organismos habitantes da cavidade bucal e sistemas interligados. Assim, os dentes com necrose pulpar e áreas radiolúcidas periapicais apresentam inúmeros morfotipos microbiológicos no sistema de canais radiculares. Não obstante, de longa data, tem sido questionada a sobrevivência de micro-organismos nos granulomas periapicais. Nesta revisão, verificou-se que na atualidade, pela técnica da cultura microbiológica ou por métodos moleculares, cocos, bacilos e filamentosos, gram-negativos e positivos, predominantemente anaeróbios estritos, identificados no sistema de canais radiculares, também são recuperados na região periapical. Esses micro-organismos também são identificados pela coloração de Brown e Brenn ou pela microscopia eletrônica de varredura no ápice radicular e na estrutura da lesão, numa frequência que oscila de 4% a 100%. Estruturalmente, organizam-se num material amorfo, iodoso, de composição polissacarídica - os biofilmes periapicais.
Starting from the pulp necrosis the pulp cavity becomes progressively colonized by several microorganisms' inhabitants of the oral cavity and interconectives systems. Thus, the teeth with pulp necrosis and radiolucencies periapical areas present countless microbiological morfotypes in the root canal system. Nevertheless, of long date, the survival of the microorganisms in the periapical granuloms has been questioned. In this review it was verified that currently, by culture or molecular methods that the coccus, bacilli and filamentous, gram-negative and positive, predominantly strict anaerobes, identified in the root canal system also are recovered in the periapical lesions. Such microorganisms are also identified for the coloration of Brown and Brenn or for the scanning electronic microscopy in the root apex and in the structure of the lesion, in a frequency that oscillates from 4% to 100%. Structurally, they are organized in an amorphous material, muddy, of polysaccharide composition- the periapical biofilms.
RESUMO
OBJETIVO: Verificar as implicações do uso do piercing lingual em indivíduos da cidade de Araraquara, São Paulo. MÉTODOS: A cidade de Araraquara, São Paulo possui cinco estabelecimentos para colocação de piercing, dos quais apenas dois autorizaram a realização da pesquisa e permitiram acesso a listagem dos usuários de piercing lingual. Os usuários foram contatados pelo telefone. Ao final do estudo a amostra esteve composta por 100 indivíduos de ambos os sexos, maiores de 18 anos, que fazem ou fizeram uso do piercing lingual. Para levantamento das informações utilizou-se um formulário, com seis questões objetivas. Os dados obtidos foram analisados por meio de estatística descritiva. RESULTADOS: Dos participantes, 77 indivíduos usavam piercing lingual há mais de seis meses. O tempo de cicatrização após a colocação do adereço foi abaixo de quatro meses, sendo que, 68 pessoas utilizaram enxaguatórios bucais e 19, além deste, utilizaram analgésicos. Apenas 11 pessoas não apresentaram sintomas após as primeiras semanas decorrentes da colocação do piercing e 56 indivíduos relataram complicações como aumento da salivação, dificuldade na fala, fraturas dentárias, traumas na gengiva ou mucosa e dificuldades na alimentação. CONCLUSÃO: Frente às implicações apresentadas pelo uso do piercing lingual justifica-se o despendimento de esforços na orientação de jovens frente à opção de utilização de piercing na cavidade oral.
OBJECTIVE: Investigated the consequences of tongue piercing in individuals from the city of Araraquara, São Paulo, Brazil. METHODS: In Araraquara there are five piercing shops, but only two agreed to help the research by providing the contact information of customers who had had their tongues pierced. These customers were contacted by telephone. By the end of the research, the sample consisted of 100 individuals from both genders aged 18 years or more. Not all of them still had their tongue piercing. A form with six objective questions was used to collect information. The information was then analyzed by descriptive statistics. RESULTS: From the 100 participants, 77 had been using tongue piercing for more than 6 months. It took less than 4 months for the tongue to heal. Sixty-eight individuals used oral rinses and of these, 19 also used painkillers. Only 11 individuals did not present symptoms after the first weeks using the piercing. On the other hand, 56 individuals reported complications such as increased salivation, speaking difficulties, gingival or mucosal traumas, dental fractures and eating difficulties. CONCLUSION: All these tongue-piercing complications justify efforts to inform the youth about the use of piercing in the oral cavity.
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Saúde Bucal , Piercing Corporal , Piercing Corporal/efeitos adversos , Fatores Etários , Inquéritos e Questionários , Fatores SexuaisRESUMO
É um desafio o tratamento ortodôntico da Classe Il subdivisão devido à relação oclusal assimétrica (Classe II de um lado da arcada dentária e Classe I do outro) e a dificuldade em diagnosticar seus fatores etiológicos. Com o objetivo de facilitar ao ortodontista o tratamento da Classe II subdivisão foi realizada uma pesquisa sobre a etiologia desta má-oclusão. Foram pesquisados vários bancos de dados eletrônicos (Pubmed, Medline, Biblioteca Cochrane, Scopus) e realizadas pesquisas secundárias. Osdados coletados demonstraram que nesta má-oclusão existe uma predominância de alterações dentoalveolares que envolvemprincipalmente o arco inferior, com o molar inferior posicionado mais posterior no lado da Classe II e um desvio de linha média dentária inferior para o lado da Classe II.Secundariamente existe uma assimetria no arco superior, com molar mais mesializado no lado da Classe II e um desvio de linha média dentária para o lado da Classe I. Portanto, a Classe II subdivisão tem uma etiologia primordialmente dentoalveolar inferior, secundariamente dentoalveolar superior e casualmente esquelética. Quandopresente, a alteração esquelética é suave e compromete principalmente a mandíbula.
The orthodontic treatment of the Class II subdivision malocclusion is challenging due to the asymmetrical occlusal relationship (Class II on one side of the dental arch and Class I on the other side) and to the complexity in the diagnosis of its etiological factors. The objective of the actual study was to bring information that facilitates the orthodontist to accomplish the Class II subdivisionmalocclusion treatment. Electronic data sources available were examined (Pubmed, Medline, Cochrane Library, Scopus) as well as secondary sources searched. The data collected indicate that in this malocclusion there is a predominance of dentoalveolaralterations which involve mainly the mandibular arch with the mandibular molar positioned more posterior on the side of the Class II and a shift of the mandibular dental medline towards the side of the Class II. Secondary there was an. asymmetry in the maxillaryarch, with greater mesial migration of the maxillary molar on the side of the Class II and a dental midline shift towards the side of the Class I. Therefore, the etiology of the Class II subdivision malocclusion is primarily dentoaveolar mandibular; secondarilydentoalveolar maxillary and occasionally skeletal. When the skeletal asymmetry is present, it is slight and it mainly compromises the mandible.