RESUMO
La epistaxis es un síntoma muy común es muestra práctica diaria, aproximadamente el 60% de la población ha tenido al menos un episodio en algún momento de su vida pero solo 6% precisó atención médica. La mayoría de episodios son limitados y benignos pero, en ocasiones, nos encontramos con casos que pueden resultar fatales.
Nosebleed is a really common symptom, about 60% of the population has had at least one episode at some point in their lives but only 6% required medical attention. Most episodes are limited and benign but in some rare cases, it could be deathly.
Assuntos
Humanos , Masculino , Adulto , Aneurisma Intracraniano/complicações , Aneurisma Intracraniano/terapia , Epistaxe/etiologia , Epistaxe/terapia , Embolização TerapêuticaRESUMO
Zinc exists in biological systems as bound and histochemically reactive free Zn(2+) in the nanomolar range. Zinc is required as either structural or catalytic component for a large number of enzymes. It also modulates current passage through many ion channels. Here, we reinvestigated the effects of extracellular and intracellular Zn(2+) on the L-type Ca(2+) current (I (CaL)) and its modulation by ß-adrenergic stimulation in rat ventricular cardiomyocytes. In the absence of Ca(2+) ions, Zn(2+) could permeate through the L-type channel at much lower concentrations and at a more positive voltage range, but with a lower permeability than Ca(2+). In the presence of Ca(2+), extracellular Zn(2+) demonstrated strong bimodal inhibitory effects on the I (CaL), with half-inhibition occurring around 30 nM, i.e., in the range of concentrations found in the plasma. Intracellular Zn(2+) also significantly inhibited the I (CaL) with a half-inhibitory effect at 12.7 nM. Moreover, ß-adrenergic stimulation was markedly reduced by intracellular Zn(2+) at even lower concentrations (<1 nM) as a consequence of Zn(2+)-induced inhibition of the adenylyl cyclase. All these effects appeared independent of redox variations and were not affected by dithiothreitol. Thus, both basal intracellular and extracellular Zn(2+) modulate transmembrane Ca(2+) movements and their regulation by ß-adrenergic stimulation. Considering that, in many pathological situations, including diabetes, the extracellular Zn(2+) concentration is reduced and the intracellular one is increased, our results help to explain both Ca(2+) overload and marked reduction in the ß-adrenergic stimulation in these diseases.
Assuntos
Canais de Cálcio Tipo L/fisiologia , Miócitos Cardíacos/fisiologia , Zinco/metabolismo , Agonistas Adrenérgicos beta/farmacologia , Animais , Cálcio/metabolismo , Canais de Cálcio Tipo L/efeitos dos fármacos , Cardiopatias/metabolismo , Ventrículos do Coração/citologia , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Ratos , Zinco/farmacologiaRESUMO
Physalia physalis is a marine cnidarian from which high molecular weight toxins with hemolytic and neurotoxic effects have been isolated. In the present work, two novel toxins, PpV9.4 and PpV19.3 were purified from P. physalis by bioactive guideline isolation. It involved two steps of column chromatography, gel filtration and RP-HPLC. The molecular weights were 550.7 and 4720.9 Da for PpV9.4 and PpV19.3, respectively. In the light of the Edman sequencing results, the structure of these toxins included the presence of modified amino acids. Both toxins increased the percentage of insulin secreting beta-cells and induced cytosolic Ca2+ elevation. To date, this is the first report of low molecular weight toxins increasing insulin secretion purified from cnidarians, by constituting a new approach to the study of beta-cells physiology.
Assuntos
Cálcio/metabolismo , Hidrozoários/metabolismo , Células Secretoras de Insulina/efeitos dos fármacos , Insulina/metabolismo , Toxinas Biológicas/farmacologia , Animais , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Cromatografia em Gel , Cromatografia de Fase Reversa , Hemólise/efeitos dos fármacos , Secreção de Insulina , Células Secretoras de Insulina/metabolismo , Ratos , Ratos Wistar , Toxinas Biológicas/isolamento & purificaçãoRESUMO
Los campos clínicos son un pilar fundamental en la formación de profesionales de la salud. Contar con campos clínicos pertinentes constituye una exigencia para el desarrollo de un proyecto educativo de calidad. Este estudio de corte transversal tiene como propósito evaluar el impacto en la formación y desarrollo profesionales, del modelo de integración docente asistencial desarrollado entre la Escuela de Enfermería de la Pontificia Universidad Católica de Chile y los Servicios de Enfermería de la Red de Salud UC (CIDAE-UC). Resultados: Es un modelo de trabajo que facilita el aprovechamiento de conocimientos y capacidades de enfermeras docentes y clínicas en la asistencia y la docencia, lo que se traduce en un incremento de docencia en las áreas clínicas y una mayor congruencia entre las necesidades de formación y las áreas clínicas identificadas para dar respuesta a ello. Es una estrategia de valor para preparar un personal idóneo en las diversas especialidades e incrementar la disciplina de enfermería, a través del desarrollo profesional y de la investigación colaborativa.
Clinical fields are a fundamental pillar in the training of health professionals. Counting on relevant clinical fields is a requirement for the development of an educational project of quality. This cross-sectional study aims to assess the impact in the training and on professional development of integration teaching healthcare model developed between the School of Nursing, Catholic University of Chile and the nursing services of the network of SALUD UC (CIDAE-UC). Results: working model that facilitates the use of knowledge and skills of teachers and clinical nurses in teaching and assistance, resulting in an increase in teaching in the clinical areas and a greater congruence between the needs of training and clinical areas identified to respond to this. It is a strategy of value to prepare an appropriate personnel in various specialties and increase the discipline of nursing, through professional development and collaborative research.
Assuntos
Humanos , Masculino , Feminino , Educação em Enfermagem , Enfermagem , Serviços de Integração Docente-Assistencial , ChileRESUMO
High polyphenol and polysaccharide levels in plant tissues such as banana fruit and leaves constitute a significant challenge to the extraction of sufficient amounts of high-quality RNA required for cDNA library synthesis and molecular analysis. To determine their comparative effectiveness at eliminating polyphenols, polysaccharides and proteins, three protocols for RNA extraction from in vitro banana plantlet leaves were tested: Concert(TM) Plant RNA isolation kit, a small-scale protocol based on Valderrama-Cháirez, and a modified version of the Valderrama-Cháirez protocol. RNA quantity and purity were evaluated by UV-spectrophotometry using DEPC-treated water and Tris-HCl, pH 7.5. Purity was greater using Tris-HCl. The Concert(TM) Plant protocol produced the poorest quality RNA. Reverse transcription into cDNAs from RNA isolated from in vitro banana plantlet leaves infected with Mycosphaerella fijiensis using the modified Valderrama-Cháirez protocol, followed by PCR using primers designed against gamma-actin from banana and M. fijiensis, yielded products of the anticipated size. In addition, this protocol reduced the processing time, lowered costs, used less expensive equipment, and could be used for other plants that have the same problems with high polyphenol and polysaccharide levels.
Assuntos
Ascomicetos/patogenicidade , Musa/genética , Musa/microbiologia , Folhas de Planta/genética , Folhas de Planta/microbiologia , RNA de Plantas/química , RNA de Plantas/isolamento & purificação , Flavonoides/química , Fenóis/química , Reação em Cadeia da Polimerase , Polifenóis , Polissacarídeos/químicaRESUMO
AIM: To determine the frequency and clinically related factors for recurrences in toxoplasmic retinochoroiditis in Colombian patients. METHODS: Cross-sectional analysis based on clinical charts of patients examined during the period of September 2005 to July 2008 at the University medical centre in Quindio (Colombia). Patients with retinochoroidal lesions consistent with Toxoplasma infection were included. Comparisons were made with an index of recurrences adjusted for months of follow-up or of the available data of periods with and without recurrences RESULTS: The clinical charts of 56 patients were analysed. In total, 25 patients (44%) were seen during an active episode, and 31 patients during inactive periods. There were 25 patients (44%) without episodes of recurrence. The total number of recurrences was 80 episodes. The mean number of recurrences was of two recurrences each 11 years. Adjusted recurrences index indicated that the most important factors associated with recurrence were previous therapy with steroids without antibiotics and previous subconjunctival injection of steroids. CONCLUSIONS: The use of systemic steroids without antibiotics and subconjunctival injection of steroids were identified as the main factors related to recurrence in this group of patients.
Assuntos
Coriorretinite/etiologia , Toxoplasmose Ocular/etiologia , Adolescente , Adulto , Antibacterianos/administração & dosagem , Criança , Pré-Escolar , Coriorretinite/tratamento farmacológico , Coriorretinite/parasitologia , Estudos Transversais , Feminino , Glucocorticoides/administração & dosagem , Humanos , Lactente , Injeções , Masculino , Pessoa de Meia-Idade , Prednisolona/administração & dosagem , Recidiva , Fatores de Risco , Fatores Socioeconômicos , Fatores de Tempo , Toxoplasmose Ocular/tratamento farmacológico , Adulto JovemRESUMO
Ibera wetland is one of the most pristine and singular ecosystems of South America. Satellite-derived thermal analysis of its lacustrine system supported the existence of a groundwater connection with the Yacyreta reservoir through basaltic fractures located along the ancient river bed of the Parana. The groundwater outflow would be located on the geological discontinuity of the eastern border of Ibera wetland, concretely Laguna Ibera and Laguna Luna-Disparo. This new sign of connection comes on top of the hydrological signs which already exist. A call to prudence recommends not extending the recently projected increase of the water level of Yacyreta reservoir. Deeper hydrogeological studies should analyze the hypothesis emerged from this study before modifying the water level of Yacyreta.
Assuntos
Ecossistema , Geologia , Abastecimento de Água , Argentina , Conservação dos Recursos Naturais , Água Doce , Fenômenos Geológicos , Humanos , ParaguaiRESUMO
Formação de um sistema de laboratório semi-automático com um suporte analítico-instrumental sem afetar os critérios de reprudutibilidade estabelecidos para os ensaios clássicos e os níveis essenciais de qualidade interna e externa
Assuntos
Humanos , Masculino , Feminino , Síndrome da Imunodeficiência Adquirida , Infecções Sexualmente Transmissíveis/diagnóstico , HIV , LaboratóriosRESUMO
We tested four isolates of Trypanosoma cruzi to assess parasite virulence and ability to cause myocarditis, cardiac sympathetic denervation, and histopathologic alterations in organs of the digestive system. The susceptibility of rats depends on the population of T. cruzi, with the ABC strain and the CL-Brener clone killing all animals, regardless of the parasitemic pattern. All tested T. cruzi populations caused acute myocarditis, but failed to induce histologic alterations in the intestine. The Cl-Brener and ABC isolates caused esophageal myositis. The myocarditis caused by the ABC, CL-Brener, and Y isolates was severe, but resolution started at the end of the acute phase. In contrast, the Col 1.7 G2 clone induced mild and sustained myocarditis. Our results also showed that T. cruzi populations able to induce severe acute myocarditis caused marked sympathetic denervation, but recovery of normal cardiac histology and innervation occurred. The sustained myocarditis induced by Col 1.7 G2 clone failed to cause sustained denervation.