RESUMO
Hemotrophic mycoplasmas (hemoplasmas), Bartonella sp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas, Bartonella sp., Hepatozoon sp. and Cytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) for Mycoplasma haemofelis, 12 (6.7%) for 'Candidatus M. haemominutum' and one (0.5%) for 'Candidatus M. turicensis'. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, 'Candidatus M. haemominutum' and 'Candidatus M. turicensis', based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showed Cytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas and Bartonella species.
Assuntos
Vetores Artrópodes , Gatos/sangue , DNA Bacteriano/sangue , Mycoplasma/genética , Mycoplasma/isolamento & purificação , Animais , Brasil , Feminino , MasculinoRESUMO
Hemotrophic mycoplasmas (hemoplasmas), Bartonellasp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas, Bartonella sp., Hepatozoon sp. and Cytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) for Mycoplasma haemofelis, 12 (6.7%) for 'Candidatus M. haemominutum' and one (0.5%) for 'Candidatus M. turicensis'. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, 'Candidatus M. haemominutum' and 'Candidatus M. turicensis', based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showed Cytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas and Bartonella species.
Micoplasmas hemotróficos (hemoplasmas), Bartonellasp., Hepatozoon sp. e Cytauxzoon felis se destacam como importantes patógenos que circulam entre gatos e hospedeiros invertebrados. O presente estudo objetivou detectar e, posteriormente confirmar por seqüenciamento, a presença de DNA de hemoplasmas, Bartonella sp., Hepatozoon sp. e Cytauxzoon felis em amostras de sangue de gatos de Cuiabá, MT, Brasil. Entre fevereiro/2009 e fevereiro de 2011, amostras de sangue acrescidas de EDTA foram coletadas de 163 gatos mantidos em quatro diferentes abrigos na cidade de Cuiabá, estado do Mato Grosso, Brasil, e de 15 gatos atendidos no Hospital Veterinário da Universidade Federal do Mato Grosso (UFTM). Dos 178 gatos amostrados, 15 (8,4%) foram positivos para hemoplasmas: quatro (2,2%) para Mycoplasma haemofelis, 12 (6,7%) para 'Candidatus M. haemominutum' e um (0,5%) para 'Candidatus M. turicensis'. Um (0.5%) gato, atendido no Hospital Veterinário da UFMT, estava co-infectado com M. haemofelis, 'Candidatus M. haemominutum' e 'Candidatus M. turicensis', baseado na confirmação por sequenciamento. Quatro gatos mostraram-se positivos para Bartonella spp.: três (1,7%) para B. henselae e um (0.5%) para B. clarridgeiae. Todos os gatos amostrados mostraram-se negativos para Cytauxzoon sp. e Hepatozoon sp. Este estudo mostrou que gatos mantidos em abrigos na cidade de Cuiabá, estado do Mato Grosso, são expostos a hemoplasmas e espécies de Bartonella sp.
Assuntos
Animais , Masculino , Feminino , Bartonella , Vetores Artrópodes , Gatos/sangue , DNA Bacteriano/sangue , Mycoplasma/genética , Mycoplasma/isolamento & purificação , Reação em Cadeia da Polimerase , BrasilRESUMO
In order to investigate new hosts for Anaplasmataceae agents in Brazil, we collected blood samples from 21 wild birds. Using molecular techniques, we detected the presence of Anaplasma phagocytophilum, Ehrlichia chaffeensis, and an Ehrlichia species closely related to Ehrlichia canis in carnivorous avian blood samples. In addition, an Ehrlichia species closely related to an Ehrlichia species found in wild felines in Brazil was also detected in a goose blood sample. Wild birds may play a role as carriers of Anaplasmataceae agents in Brazil.