RESUMO
Actualmente, es frecuente la consulta por trastornos temporomandibulares (TTM) en la clínica odontopediátrica, siendo reconocida la múltiple causalidad de los mismos. Se presenta la resolución de una situación clínica de una paciente de 13 años de edad que acudió a la Cátedra de Odontología Integral Niños (OIN), presentando dolor muscular y articular del lado derecho, limitando la apertura bucal a 25 mm confortable y a 28 mm forzada, con desvío de la mandíbula hacia la izquierda. Refería haber sido atendida en una guardia médica el día anterior con bloqueo en apertura, luego de 8 meses de evolución, con síntomas de chasquido y doloir, sin haber realizado consulta alguna. Se indicó tratamiento sintomático y se solicitaron estudios complementarios. El diagnóstico fue de luxación discal sin reducción. Con la evaluación integral, se hallaron factores concomitantes, tales como maloclusión, hiperlaxitud, respiración bucal y parafunciones. En el abordaje terapéutico, se colocó un intermediario oclusal para reposicionamiento mandibular, se realizó tratamiento fonoaudiológico miofuncional y reeducación postural global (RPG). La paciente evolucionó favorablemente; a los 6 meses resolvió el ruido articular y al año estaba asintomática, con 37 mm de apertura, comenzando su tratamiento de ortodoncia y manteniendo controles durante 4 años. El compromiso de la familia y la participación de un equipo de trabajo, permitió la resolución integral del caso. El odontopediatra tiene la responsabilidad de alertar a padres y pacientes sobre factores de riesgo, diagnosticar TTM y orientar los tratamientos, involucrándose cuando corresponde su intervención, o derivando en forma oportuna...
Assuntos
Humanos , Adolescente , Feminino , Assistência Odontológica para Crianças/métodos , Deslocamento do Disco Intervertebral/terapia , Disco da Articulação Temporomandibular/lesões , Ferula , Odontopediatria/tendências , Argentina , Deslocamento do Disco Intervertebral/diagnóstico , Faculdades de Odontologia , Má Oclusão/diagnóstico , Ortodontia Corretiva/métodos , Equipe de Assistência ao Paciente , Postura/fisiologia , Respiração Bucal/diagnóstico , Resultado do Tratamento , Terapia Miofuncional/métodosRESUMO
UNLABELLED: There is evidence that acute myocardial infarction (AMI) is associated with increasing production of reactive oxygen species and tissue injury. The aim of this study was to assess the presence of oxidative stress indices in saliva 24 and 48h after AMI. MATERIALS AND METHODS: We designed a prospective study comparing salivary levels of biomarkers of oxidative stress in patients with AMI with elevation of the ST segment in electrocardiogram versus clinically healthy subjects. Oxidative stress indices including the rate of oxidation of 2'7' dichlorohydrofluorescein diacetate (DCFH-DA) and the activity of the antioxidant enzyme catalase (CAT) were evaluated in saliva from patients with AMI at 24 and 48 hours. At each sampling time, blood was drawn for serum markers of myocardial infarction. RESULTS: This study included ten patients with acute ST-segment elevation myocardial infarction and ten clinically healthy controls. Mean age was 67.8 +/- 11.1 vs. 48.7 +/- 4.1 years (p < 0.001) and gender was 60% male vs. 50% (p > 0.05) for AMI vs. controls, respectively. Our results demonstrated an increase in the rate of oxidation of DCFH-DA in the myocardial infarction group as compared with controls (p = 0.004), which remained unchanged at 48h. There was no difference in salivary catalase activity between controls and AML subjects at 24h or at 48h post-diagnosis (p = 0.157). The relationship between CAT48 and DCFH-DA48 was fairly significant (r = 0.39; p = 0.053). CONCLUSION: This preliminary study showed that biomarkers of oxidative stress are detectable in saliva of patients with acute myocardial infarction. CLINICAL RELEVANCE: Future studies using a larger population are needed to confirm these observations and to explore the possibility of using the saliva to monitor evolving diagnosis and prognosis in acute coronary syndrome.
Assuntos
Infarto do Miocárdio/metabolismo , Estresse Oxidativo , Saliva/química , Idoso , Biomarcadores/análise , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos ProspectivosRESUMO
UNLABELLED: There is evidence that acute myocardial infarction (AMI) is associated with increasing production of reactive oxygen species and tissue injury. The aim of this study was to assess the presence of oxidative stress indices in saliva 24 and 48h after AMI. MATERIALS AND METHODS: We designed a prospective study comparing salivary levels of biomarkers of oxidative stress in patients with AMI with elevation of the ST segment in electrocardiogram versus clinically healthy subjects. Oxidative stress indices including the rate of oxidation of 27 dichlorohydrofluorescein diacetate (DCFH-DA) and the activity of the antioxidant enzyme catalase (CAT) were evaluated in saliva from patients with AMI at 24 and 48 hours. At each sampling time, blood was drawn for serum markers of myocardial infarction. RESULTS: This study included ten patients with acute ST-segment elevation myocardial infarction and ten clinically healthy controls. Mean age was 67.8 +/- 11.1 vs. 48.7 +/- 4.1 years (p < 0.001) and gender was 60
male vs. 50
(p > 0.05) for AMI vs. controls, respectively. Our results demonstrated an increase in the rate of oxidation of DCFH-DA in the myocardial infarction group as compared with controls (p = 0.004), which remained unchanged at 48h. There was no difference in salivary catalase activity between controls and AML subjects at 24h or at 48h post-diagnosis (p = 0.157). The relationship between CAT48 and DCFH-DA48 was fairly significant (r = 0.39; p = 0.053). CONCLUSION: This preliminary study showed that biomarkers of oxidative stress are detectable in saliva of patients with acute myocardial infarction. CLINICAL RELEVANCE: Future studies using a larger population are needed to confirm these observations and to explore the possibility of using the saliva to monitor evolving diagnosis and prognosis in acute coronary syndrome.
Assuntos
Infarto do Miocárdio/metabolismo , Estresse Oxidativo , Saliva/química , Idoso , Biomarcadores/análise , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos ProspectivosRESUMO
Existe evidencia que permite establecer una asociación entre la generación de especies reactivas del oxígeno y el daño tisular en el síndrome coronario agudo. El objetivo de este trabajo fue detectaren saliva de pacientes con infarto agudo de miocardio (IAM), la presencia de reactantes de estrés oxidativo a las 24 y 48 horas. Materiales y métodos: se efectuó un estudio prospectivo de comparación entre pacientes con IAM con supradesnivel del segmento STen el electrocardiograma y sujetos sin patología clínica evidente. Laproducción de especies reactivas de oxígeno fue evaluada mediantela tasa de oxidación de la 2Æ7Æ diacetato de diclorohidrofluoreceina (DCFH-DA) y la actividad antioxidante de la enzima catalasa (CAT) en saliva de pacientes con IAM a las 24 y 48 h de producido el síndrome coronario agudo. Simultáneamente, se determinaron en suero los biomarcadores diagnósticos de IAM. Resultados: se incorporaron10 pacientes con IAM con supradesnivel del ST que fueron comparados con 10 sujetos del grupo control. La edad promedio fue 67.8 ± 11.1 vs 48.7 ± 4.1 años, respectivamente (p<0.001); el 60 por ciento vs 50 por ciento fueron hombres sin diferencias entre ambos grupos (p>0.05). La media de la velocidad de oxidación de la DCFH-DA fue mayor a las 24 h en los pacientes con IAM (p=0.004). Estas diferencias semantuvieron a las 48 h del infarto sin cambios significativos. No se encontraron diferencias en las medias de actividad de la enzima catalasa entre IAM y control (p>0.05). Se encontró una relación entre CAT48 y DCFH-DA48 (r=0.39; p=0.053). Conclusiones: Enesta población se han detectado reactantes de estrés oxidativo ensaliva de pacientes con IAM. Relevancia clínica: nuevos estudios con mayor número de casos serán necesarios para confirmar estas observaciones y evaluar la utilidad de la saliva en el diagnóstico,evolución y pronóstico del síndrome coronario agudo.(AU)
There is evidence that acute myocardial infarction (AMI) is asso-ciated with increasing production of reactive oxygen species andtissue injury. The aim of this study was to assess the presence ofoxidative stress indices in saliva 24 and 48h after AMI. Materialsand methods: We designed a prospective study comparing sali-vary levels of biomarkers of oxidative stress in patients with AMIwith elevation of the ST segment in electrocardiogram versus clin-ically healthy subjects. Oxidative stress indices including the rateof oxidation of 2Æ7Æ dichlorohydrofluorescein diacetate (DCFH-DA) and the activity of the antioxidant enzyme catalase (CAT)were evaluated in saliva from patients with AMI at 24 and 48hours. At each sampling time, blood was drawn for serum mark-ers of myocardial infarction. Results: This study included tenpatients with acute ST-segment elevation myocardial infarctionand ten clinically healthy controls. Mean age was 67.8 ± 11.1 vs.48.7 ± 4.1 years (p<0.001) and gender was 60% male vs. 50%(p>0.05) for AMI vs. controls, respectively. Our results demon-strated an increase in the rate of oxidation of DCFH-DA in themyocardial infarction group as compared with controls (p=0.004),which remained unchanged at 48h. There was no difference insalivary catalase activity between controls and AMI subjects at24h or at 48h post-diagnosis (p=0.157). The relationship betweenCAT48and DCFH-DA48was fairly significant (r=0.39; p=0.053).Conclusion: This preliminary study showed that biomarkers ofoxidative stress are detectable in saliva of patients with acutemyocardial infarction. Clinical Relevance: Future studies using alarger population are needed to confirm these observations and toexplore the possibility of using the saliva to monitor evolving diag-nosis and prognosis in acute coronary syndrome.(AU)
Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Infarto do Miocárdio/metabolismo , Saliva/química , Biomarcadores , Estudos ProspectivosRESUMO
Existe evidencia que permite establecer una asociación entre la generación de especies reactivas del oxígeno y el daño tisular en el síndrome coronario agudo. El objetivo de este trabajo fue detectaren saliva de pacientes con infarto agudo de miocardio (IAM), la presencia de reactantes de estrés oxidativo a las 24 y 48 horas. Materiales y métodos: se efectuó un estudio prospectivo de comparación entre pacientes con IAM con supradesnivel del segmento STen el electrocardiograma y sujetos sin patología clínica evidente. Laproducción de especies reactivas de oxígeno fue evaluada mediantela tasa de oxidación de la 27 diacetato de diclorohidrofluoreceina (DCFH-DA) y la actividad antioxidante de la enzima catalasa (CAT) en saliva de pacientes con IAM a las 24 y 48 h de producido el síndrome coronario agudo. Simultáneamente, se determinaron en suero los biomarcadores diagnósticos de IAM. Resultados: se incorporaron10 pacientes con IAM con supradesnivel del ST que fueron comparados con 10 sujetos del grupo control. La edad promedio fue 67.8 ± 11.1 vs 48.7 ± 4.1 años, respectivamente (p<0.001); el 60 por ciento vs 50 por ciento fueron hombres sin diferencias entre ambos grupos (p>0.05). La media de la velocidad de oxidación de la DCFH-DA fue mayor a las 24 h en los pacientes con IAM (p=0.004). Estas diferencias semantuvieron a las 48 h del infarto sin cambios significativos. No se encontraron diferencias en las medias de actividad de la enzima catalasa entre IAM y control (p>0.05). Se encontró una relación entre CAT48 y DCFH-DA48 (r=0.39; p=0.053). Conclusiones: Enesta población se han detectado reactantes de estrés oxidativo ensaliva de pacientes con IAM. Relevancia clínica: nuevos estudios con mayor número de casos serán necesarios para confirmar estas observaciones y evaluar la utilidad de la saliva en el diagnóstico,evolución y pronóstico del síndrome coronario agudo.
There is evidence that acute myocardial infarction (AMI) is asso-ciated with increasing production of reactive oxygen species andtissue injury. The aim of this study was to assess the presence ofoxidative stress indices in saliva 24 and 48h after AMI. Materialsand methods: We designed a prospective study comparing sali-vary levels of biomarkers of oxidative stress in patients with AMIwith elevation of the ST segment in electrocardiogram versus clin-ically healthy subjects. Oxidative stress indices including the rateof oxidation of 27 dichlorohydrofluorescein diacetate (DCFH-DA) and the activity of the antioxidant enzyme catalase (CAT)were evaluated in saliva from patients with AMI at 24 and 48hours. At each sampling time, blood was drawn for serum mark-ers of myocardial infarction. Results: This study included tenpatients with acute ST-segment elevation myocardial infarctionand ten clinically healthy controls. Mean age was 67.8 ± 11.1 vs.48.7 ± 4.1 years (p<0.001) and gender was 60% male vs. 50%(p>0.05) for AMI vs. controls, respectively. Our results demon-strated an increase in the rate of oxidation of DCFH-DA in themyocardial infarction group as compared with controls (p=0.004),which remained unchanged at 48h. There was no difference insalivary catalase activity between controls and AMI subjects at24h or at 48h post-diagnosis (p=0.157). The relationship betweenCAT48and DCFH-DA48was fairly significant (r=0.39; p=0.053).Conclusion: This preliminary study showed that biomarkers ofoxidative stress are detectable in saliva of patients with acutemyocardial infarction. Clinical Relevance: Future studies using alarger population are needed to confirm these observations and toexplore the possibility of using the saliva to monitor evolving diag-nosis and prognosis in acute coronary syndrome.
Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Infarto do Miocárdio/metabolismo , Saliva/química , Biomarcadores , Estudos ProspectivosRESUMO
The effect of in vivo Fe exposure on the oxidative metabolism of the bivalve Myaarenaria was studied. Fe was supplemented in natural seawater and resulted in a significant increase in the total Fe content in the bivalve digestive gland (DG) between 9 to 17days of exposure. Mortality of treated animals increased drastically after day 18. Oxidative stress conditions were characterized in DG through assessment of the generation of reactive oxygen species (ROS) and ascorbyl radical (A) content. Both parameters were affected following a biphasic profile showing significant increases by days 2 and 9 of Fe exposure. The content of 2-thiobarbituric acid reactive substances (TBARS) was significantly increased over control values by days 2, 9 and 17 of treatment. The labile Fe pool (LIP) in isolated DG was elevated over control values by day 7, and maintained this increase until day 17 of Fe exposure. The content of NO, assessed by EPR spin trapping, was 60% lower in DG of animals exposed for 2days to Fe than in control values, with no further changes. The biphasic profile of oxidative stress response to Fe exposure in DG suggests that at early stages of Fe supplementation the cellular control mechanisms, such as CAT activity, were operative to limit oxidative damage, but further Fe exposure overwhelmed these abilities. Moreover, the second phase could be understood as the consequence of the exhaustion of cellular protective systems that could also involve NO.